Molecular prevalence and phylogenetic confirmation of bovine trichomoniasis in aborted cows in Iraq
Background and Aim: Bovine trichomoniasis, caused by Tritrichomonas foetus, is a venereal disease that is distributed in many countries, including Iraq. Compared with other abortive infectious diseases, prevalence of T. foetus is expected to be relatively low in the field by veterinarians. This stud...
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| Format: | Article |
| Language: | English |
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Veterinary World
2023-03-01
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| Series: | Veterinary World |
| Subjects: | |
| Online Access: | https://www.veterinaryworld.org/Vol.16/March-2023/19.pdf |
| _version_ | 1797864504384552960 |
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| author | Hasanain A. J. Gharban |
| author_facet | Hasanain A. J. Gharban |
| author_sort | Hasanain A. J. Gharban |
| collection | DOAJ |
| description | Background and Aim: Bovine trichomoniasis, caused by Tritrichomonas foetus, is a venereal disease that is distributed in many countries, including Iraq. Compared with other abortive infectious diseases, prevalence of T. foetus is expected to be relatively low in the field by veterinarians. This study aimed to estimate the prevalence of T. foetus in aborted cows by conventional polymerase chain reaction (PCR) and phylogenetic analysis of local T. foetus isolates was documented in the National Center for Biotechnology Information as the first sequenced isolates from Iraq.
Materials and Methods: Vaginal fluids were collected from 62 aborted cows and examined by PCR. Data were reported for the following parameters: Vital signs (body temperature and respiratory and pulse rates), age (<4, 4–8, and >8 years), reproductive health status (premature calving, embryonic death, pyometra, and healthy newborn), breed (pure or crossbred), type of breeding (natural or artificial), bull-to-cow ratio (1:<10, 1:10–20, and 1:>20), contact of cow with bull(s) from other farmers (yes or no), and contact with stray animals (dogs and cats).
Results: A total of 20.97% of aborted cows were positive for T. foetus. Phylogenetic analysis for 10 positive local T. foetus isolates demonstrated high identity with the Thai (MN560972.2) and Chinese (MH115435.1) isolates, with an identity range of 98.8%–99.5% and 98.6%–99.3%, respectively. Clinical data showed that the vital signs differed insignificantly between cows positive and negative for T. foetus. Prevalence and risk of infection increased significantly in <4-year-old, early calving, embryonic death, crossbred, and naturally inseminated cows that had direct contact with bulls from other farmers, and contact with stray animals. Fetal pneumonia and death of premature calves were significant among positive aborted fetuses.
Conclusion: Tritrichomonas foetus is highly prevalent in aborted cows in Iraq and phylogenetic analysis demonstrated an identity between the local and global isolates, that is, Thai and Chinese, of cats. |
| first_indexed | 2024-04-09T22:54:01Z |
| format | Article |
| id | doaj.art-8c56aec517e6495bb9dc1b359239855c |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-04-09T22:54:01Z |
| publishDate | 2023-03-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-8c56aec517e6495bb9dc1b359239855c2023-03-22T11:27:13ZengVeterinary WorldVeterinary World0972-89882231-09162023-03-0116358058710.14202/vetworld.2023.580-587Molecular prevalence and phylogenetic confirmation of bovine trichomoniasis in aborted cows in IraqHasanain A. J. Gharban0https://orcid.org/0000-0002-6438-1450Department of Internal and Preventive Veterinary Medicine, College of Veterinary Medicine, University of Wasit, Wasit, Iraq.Background and Aim: Bovine trichomoniasis, caused by Tritrichomonas foetus, is a venereal disease that is distributed in many countries, including Iraq. Compared with other abortive infectious diseases, prevalence of T. foetus is expected to be relatively low in the field by veterinarians. This study aimed to estimate the prevalence of T. foetus in aborted cows by conventional polymerase chain reaction (PCR) and phylogenetic analysis of local T. foetus isolates was documented in the National Center for Biotechnology Information as the first sequenced isolates from Iraq. Materials and Methods: Vaginal fluids were collected from 62 aborted cows and examined by PCR. Data were reported for the following parameters: Vital signs (body temperature and respiratory and pulse rates), age (<4, 4–8, and >8 years), reproductive health status (premature calving, embryonic death, pyometra, and healthy newborn), breed (pure or crossbred), type of breeding (natural or artificial), bull-to-cow ratio (1:<10, 1:10–20, and 1:>20), contact of cow with bull(s) from other farmers (yes or no), and contact with stray animals (dogs and cats). Results: A total of 20.97% of aborted cows were positive for T. foetus. Phylogenetic analysis for 10 positive local T. foetus isolates demonstrated high identity with the Thai (MN560972.2) and Chinese (MH115435.1) isolates, with an identity range of 98.8%–99.5% and 98.6%–99.3%, respectively. Clinical data showed that the vital signs differed insignificantly between cows positive and negative for T. foetus. Prevalence and risk of infection increased significantly in <4-year-old, early calving, embryonic death, crossbred, and naturally inseminated cows that had direct contact with bulls from other farmers, and contact with stray animals. Fetal pneumonia and death of premature calves were significant among positive aborted fetuses. Conclusion: Tritrichomonas foetus is highly prevalent in aborted cows in Iraq and phylogenetic analysis demonstrated an identity between the local and global isolates, that is, Thai and Chinese, of cats.https://www.veterinaryworld.org/Vol.16/March-2023/19.pdfbovine-sexually transmitted diseasenatural inseminationpolymerase chain reactiontritrichomonas foetuswasit province |
| spellingShingle | Hasanain A. J. Gharban Molecular prevalence and phylogenetic confirmation of bovine trichomoniasis in aborted cows in Iraq Veterinary World bovine-sexually transmitted disease natural insemination polymerase chain reaction tritrichomonas foetus wasit province |
| title | Molecular prevalence and phylogenetic confirmation of bovine trichomoniasis in aborted cows in Iraq |
| title_full | Molecular prevalence and phylogenetic confirmation of bovine trichomoniasis in aborted cows in Iraq |
| title_fullStr | Molecular prevalence and phylogenetic confirmation of bovine trichomoniasis in aborted cows in Iraq |
| title_full_unstemmed | Molecular prevalence and phylogenetic confirmation of bovine trichomoniasis in aborted cows in Iraq |
| title_short | Molecular prevalence and phylogenetic confirmation of bovine trichomoniasis in aborted cows in Iraq |
| title_sort | molecular prevalence and phylogenetic confirmation of bovine trichomoniasis in aborted cows in iraq |
| topic | bovine-sexually transmitted disease natural insemination polymerase chain reaction tritrichomonas foetus wasit province |
| url | https://www.veterinaryworld.org/Vol.16/March-2023/19.pdf |
| work_keys_str_mv | AT hasanainajgharban molecularprevalenceandphylogeneticconfirmationofbovinetrichomoniasisinabortedcowsiniraq |