A favorable suture method for size-mismatched nerve transfer: comparison with standard perineural suture in an experimental rat study
Abstract Background In nerve transfer for peripheral nerve injury, it is sometimes necessary to suture size-mismatched nerves. In 1993, a favorable suture method called the Ochiai suture method for size-mismatched nerve transfer was reported. However, there is currently a lack of substantial evidenc...
| Main Authors: | , , , , , |
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| Format: | Article |
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BMC
2023-09-01
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| Series: | Journal of Orthopaedic Surgery and Research |
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| Online Access: | https://doi.org/10.1186/s13018-023-04123-7 |
| _version_ | 1797557730264743936 |
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| author | Sho Iwabuchi Yuki Hara Yuichi Yoshii Akira Ikumi Hajime Mishima Masashi Yamazaki |
| author_facet | Sho Iwabuchi Yuki Hara Yuichi Yoshii Akira Ikumi Hajime Mishima Masashi Yamazaki |
| author_sort | Sho Iwabuchi |
| collection | DOAJ |
| description | Abstract Background In nerve transfer for peripheral nerve injury, it is sometimes necessary to suture size-mismatched nerves. In 1993, a favorable suture method called the Ochiai suture method for size-mismatched nerve transfer was reported. However, there is currently a lack of substantial evidence beyond the original report. Therefore, this study aimed to verify the advantages of using the Ochiai suture method for size-mismatched nerve transfer. Methods A total of 18 rats were evaluated in this study and randomly divided into two groups. All rats underwent femoral to sciatic nerve transfer. Specifically, group A (n = 10) underwent the Ochiai suture method, while group B (n = 8) underwent the perineural suture method. After 12 weeks postoperatively, we conducted the sciatic functional index (SFI) test, measured muscle wet-weight, and performed histological evaluations. All data were compared between the two groups, with Welch’s t test for normally distributed data and Mann-Whitney's U test for non-normally distributed data. Statistical significance was set at p < 0.05. Results The mean number of axons was significantly greater in group A than in group B at 5 mm distal to the stump (p = 0.04). Additionally, the average axonal diameter was significantly greater in group A than in group B at 5 mm and 10 mm distal to the stump (p < 0.01 and p < 0.01, respectively). However, the SFI test and measured muscle wet-weight values showed no significant differences between the two groups. Conclusions Our study revealed that the Ochiai suture method for size-mismatched nerve transfer in rats increases the regenerative axon numbers and diameters. These findings suggest that the Ochiai suture method could be a valuable approach for achieving effective motor function restoration in cases of size-mismatched nerve transfer. |
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| issn | 1749-799X |
| language | English |
| last_indexed | 2024-03-10T17:21:35Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
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| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj.art-8c595af66db04a598b767ec11afc121e2023-11-20T10:20:34ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2023-09-011811710.1186/s13018-023-04123-7A favorable suture method for size-mismatched nerve transfer: comparison with standard perineural suture in an experimental rat studySho Iwabuchi0Yuki Hara1Yuichi Yoshii2Akira Ikumi3Hajime Mishima4Masashi Yamazaki5Department of Orthopaedic Surgery, Faculty of Medicine, University of TsukubaDepartment of Orthopaedic Surgery, National Center of Neurology and PsychiatryDepartment of Orthopaedic Surgery, Tokyo Medical University Ibaraki Medical CenterDepartment of Orthopaedic Surgery, Faculty of Medicine, University of TsukubaDepartment of Orthopaedic Surgery, Faculty of Medicine, University of TsukubaDepartment of Orthopaedic Surgery, Faculty of Medicine, University of TsukubaAbstract Background In nerve transfer for peripheral nerve injury, it is sometimes necessary to suture size-mismatched nerves. In 1993, a favorable suture method called the Ochiai suture method for size-mismatched nerve transfer was reported. However, there is currently a lack of substantial evidence beyond the original report. Therefore, this study aimed to verify the advantages of using the Ochiai suture method for size-mismatched nerve transfer. Methods A total of 18 rats were evaluated in this study and randomly divided into two groups. All rats underwent femoral to sciatic nerve transfer. Specifically, group A (n = 10) underwent the Ochiai suture method, while group B (n = 8) underwent the perineural suture method. After 12 weeks postoperatively, we conducted the sciatic functional index (SFI) test, measured muscle wet-weight, and performed histological evaluations. All data were compared between the two groups, with Welch’s t test for normally distributed data and Mann-Whitney's U test for non-normally distributed data. Statistical significance was set at p < 0.05. Results The mean number of axons was significantly greater in group A than in group B at 5 mm distal to the stump (p = 0.04). Additionally, the average axonal diameter was significantly greater in group A than in group B at 5 mm and 10 mm distal to the stump (p < 0.01 and p < 0.01, respectively). However, the SFI test and measured muscle wet-weight values showed no significant differences between the two groups. Conclusions Our study revealed that the Ochiai suture method for size-mismatched nerve transfer in rats increases the regenerative axon numbers and diameters. These findings suggest that the Ochiai suture method could be a valuable approach for achieving effective motor function restoration in cases of size-mismatched nerve transfer.https://doi.org/10.1186/s13018-023-04123-7Brachial plexus injuryPeripheral nerve injuryNerve transferSuture methodNerve regenerationSize-mismatched nerve suture |
| spellingShingle | Sho Iwabuchi Yuki Hara Yuichi Yoshii Akira Ikumi Hajime Mishima Masashi Yamazaki A favorable suture method for size-mismatched nerve transfer: comparison with standard perineural suture in an experimental rat study Journal of Orthopaedic Surgery and Research Brachial plexus injury Peripheral nerve injury Nerve transfer Suture method Nerve regeneration Size-mismatched nerve suture |
| title | A favorable suture method for size-mismatched nerve transfer: comparison with standard perineural suture in an experimental rat study |
| title_full | A favorable suture method for size-mismatched nerve transfer: comparison with standard perineural suture in an experimental rat study |
| title_fullStr | A favorable suture method for size-mismatched nerve transfer: comparison with standard perineural suture in an experimental rat study |
| title_full_unstemmed | A favorable suture method for size-mismatched nerve transfer: comparison with standard perineural suture in an experimental rat study |
| title_short | A favorable suture method for size-mismatched nerve transfer: comparison with standard perineural suture in an experimental rat study |
| title_sort | favorable suture method for size mismatched nerve transfer comparison with standard perineural suture in an experimental rat study |
| topic | Brachial plexus injury Peripheral nerve injury Nerve transfer Suture method Nerve regeneration Size-mismatched nerve suture |
| url | https://doi.org/10.1186/s13018-023-04123-7 |
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