Semen Modulates the Expression of NGF, ABHD2, VCAN, and CTEN in the Reproductive Tract of Female Rabbits
Semen changes the gene expression in endometrial and oviductal tissues modulating important processes for reproduction. We tested the hypothesis that mating and/or sperm-free seminal plasma deposition in the reproductive tract affect the expression of genes associated with sperm-lining epithelium in...
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MDPI AG
2020-07-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/11/7/758 |
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| author | Jaume Gardela Amaia Jauregi-Miguel Cristina A. Martinez Heriberto Rodriguez-Martinez Manel Lopez-Bejar Manuel Alvarez-Rodriguez |
| author_facet | Jaume Gardela Amaia Jauregi-Miguel Cristina A. Martinez Heriberto Rodriguez-Martinez Manel Lopez-Bejar Manuel Alvarez-Rodriguez |
| author_sort | Jaume Gardela |
| collection | DOAJ |
| description | Semen changes the gene expression in endometrial and oviductal tissues modulating important processes for reproduction. We tested the hypothesis that mating and/or sperm-free seminal plasma deposition in the reproductive tract affect the expression of genes associated with sperm-lining epithelium interactions, ovulation, and pre-implantation effects (nerve growth factor, NGF; α/β hydrolase domain-containing protein 2, ABHD2; C-terminal tensin-like protein, CTEN or TNS4; and versican, VCAN) in the period 10–72 h post-mating. In Experiment 1, does (<i>n</i> = 9) were treated with gonadotropin-releasing hormone (GnRH) (control), GnRH-stimulated, and vaginally infused with sperm-free seminal plasma (SP-AI), or GnRH-stimulated and naturally mated (NM). In Experiment 2, does (<i>n</i> = 15) were GnRH-stimulated and naturally mated. Samples were retrieved from the internal reproductive tracts (cervix-to-infundibulum) 20 h post-treatment (Experiment 1) or sequentially collected at 10, 24, 36, 68, or 72 h post-mating (Experiment 2, 3 does/period). All samples were processed for gene expression analysis by quantitative PCR. Data showed an upregulation of endometrial <i>CTEN</i> and <i>NGF</i> by NM, but not by SP-AI. The findings suggest that the <i>NGF</i> gene affects the reproductive tract of the doe during ovulation and beyond, influencing the maternal environment during early embryonic development. |
| first_indexed | 2024-03-10T18:38:39Z |
| format | Article |
| id | doaj.art-8c5a371d3d114a6c9495504ef1dce5e7 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T18:38:39Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-8c5a371d3d114a6c9495504ef1dce5e72023-11-20T06:03:33ZengMDPI AGGenes2073-44252020-07-0111775810.3390/genes11070758Semen Modulates the Expression of NGF, ABHD2, VCAN, and CTEN in the Reproductive Tract of Female RabbitsJaume Gardela0Amaia Jauregi-Miguel1Cristina A. Martinez2Heriberto Rodriguez-Martinez3Manel Lopez-Bejar4Manuel Alvarez-Rodriguez5Division of Children’s and Women Health (BKH), Obstetrics and Gynecology, Department of Biomedical and Clinical Sciences (BKV), Linköping University, 58185 Linköping, SwedenDivision of Molecular Medicine and Virology (MMV), Linköping University, 58185 Linköping, SwedenDivision of Children’s and Women Health (BKH), Obstetrics and Gynecology, Department of Biomedical and Clinical Sciences (BKV), Linköping University, 58185 Linköping, SwedenDivision of Children’s and Women Health (BKH), Obstetrics and Gynecology, Department of Biomedical and Clinical Sciences (BKV), Linköping University, 58185 Linköping, SwedenDepartment of Animal Health and Anatomy, Veterinary Faculty, Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainDivision of Children’s and Women Health (BKH), Obstetrics and Gynecology, Department of Biomedical and Clinical Sciences (BKV), Linköping University, 58185 Linköping, SwedenSemen changes the gene expression in endometrial and oviductal tissues modulating important processes for reproduction. We tested the hypothesis that mating and/or sperm-free seminal plasma deposition in the reproductive tract affect the expression of genes associated with sperm-lining epithelium interactions, ovulation, and pre-implantation effects (nerve growth factor, NGF; α/β hydrolase domain-containing protein 2, ABHD2; C-terminal tensin-like protein, CTEN or TNS4; and versican, VCAN) in the period 10–72 h post-mating. In Experiment 1, does (<i>n</i> = 9) were treated with gonadotropin-releasing hormone (GnRH) (control), GnRH-stimulated, and vaginally infused with sperm-free seminal plasma (SP-AI), or GnRH-stimulated and naturally mated (NM). In Experiment 2, does (<i>n</i> = 15) were GnRH-stimulated and naturally mated. Samples were retrieved from the internal reproductive tracts (cervix-to-infundibulum) 20 h post-treatment (Experiment 1) or sequentially collected at 10, 24, 36, 68, or 72 h post-mating (Experiment 2, 3 does/period). All samples were processed for gene expression analysis by quantitative PCR. Data showed an upregulation of endometrial <i>CTEN</i> and <i>NGF</i> by NM, but not by SP-AI. The findings suggest that the <i>NGF</i> gene affects the reproductive tract of the doe during ovulation and beyond, influencing the maternal environment during early embryonic development.https://www.mdpi.com/2073-4425/11/7/758gene expressionqPCRfemale reproductive tractinduced ovulator |
| spellingShingle | Jaume Gardela Amaia Jauregi-Miguel Cristina A. Martinez Heriberto Rodriguez-Martinez Manel Lopez-Bejar Manuel Alvarez-Rodriguez Semen Modulates the Expression of NGF, ABHD2, VCAN, and CTEN in the Reproductive Tract of Female Rabbits Genes gene expression qPCR female reproductive tract induced ovulator |
| title | Semen Modulates the Expression of NGF, ABHD2, VCAN, and CTEN in the Reproductive Tract of Female Rabbits |
| title_full | Semen Modulates the Expression of NGF, ABHD2, VCAN, and CTEN in the Reproductive Tract of Female Rabbits |
| title_fullStr | Semen Modulates the Expression of NGF, ABHD2, VCAN, and CTEN in the Reproductive Tract of Female Rabbits |
| title_full_unstemmed | Semen Modulates the Expression of NGF, ABHD2, VCAN, and CTEN in the Reproductive Tract of Female Rabbits |
| title_short | Semen Modulates the Expression of NGF, ABHD2, VCAN, and CTEN in the Reproductive Tract of Female Rabbits |
| title_sort | semen modulates the expression of ngf abhd2 vcan and cten in the reproductive tract of female rabbits |
| topic | gene expression qPCR female reproductive tract induced ovulator |
| url | https://www.mdpi.com/2073-4425/11/7/758 |
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