Macrophages Break Interneuromast Cell Quiescence by Intervening in the Inhibition of Schwann Cells in the Zebrafish Lateral Line
In the zebrafish lateral line system, interneuromast cells (INCs) between neuromasts are kept quiescent by underlying Schwann cells (SWCs). Upon severe injuries that cause the complete loss of an entire neuromast, INCs can occasionally differentiate into neuromasts but how they escape from the inhib...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2022.907863/full |
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| author | Meng-Ju Lin Chia-Ming Lee Wei-Lin Hsu Bi-Chang Chen Shyh-Jye Lee Shyh-Jye Lee Shyh-Jye Lee |
| author_facet | Meng-Ju Lin Chia-Ming Lee Wei-Lin Hsu Bi-Chang Chen Shyh-Jye Lee Shyh-Jye Lee Shyh-Jye Lee |
| author_sort | Meng-Ju Lin |
| collection | DOAJ |
| description | In the zebrafish lateral line system, interneuromast cells (INCs) between neuromasts are kept quiescent by underlying Schwann cells (SWCs). Upon severe injuries that cause the complete loss of an entire neuromast, INCs can occasionally differentiate into neuromasts but how they escape from the inhibition by SWCs is still unclear. Using a genetic/chemical method to ablate a neuromast precisely, we found that a small portion of larvae can regenerate a new neuromast. However, the residual regeneration capacity was hindered by inhibiting macrophages. Using in toto imaging, we further discovered heterogeneities in macrophage behavior and distribution along the lateral line. We witnessed the crawling of macrophages between the injured lateral line and SWCs during regeneration and between the second primordium and the first mature lateral line during development. It implies that macrophages may physically alleviate the nerve inhibition to break the dormancy of INCs during regeneration and development in the zebrafish lateral line. |
| first_indexed | 2024-04-13T14:47:08Z |
| format | Article |
| id | doaj.art-8c601aa6b0414554aefada63eaa0688f |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-04-13T14:47:08Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-8c601aa6b0414554aefada63eaa0688f2022-12-22T02:42:43ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2022-07-011010.3389/fcell.2022.907863907863Macrophages Break Interneuromast Cell Quiescence by Intervening in the Inhibition of Schwann Cells in the Zebrafish Lateral LineMeng-Ju Lin0Chia-Ming Lee1Wei-Lin Hsu2Bi-Chang Chen3Shyh-Jye Lee4Shyh-Jye Lee5Shyh-Jye Lee6Department of Life Science, National Taiwan University, Taipei, Taiwan, R.O.C.Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan, R.O.C.Department of Life Science, National Taiwan University, Taipei, Taiwan, R.O.C.Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan, R.O.C.Department of Life Science, National Taiwan University, Taipei, Taiwan, R.O.C.Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University, Taipei, Taiwan, R.O.C.Center for Biotechnology, National Taiwan University, Taipei, Taiwan, R.O.C.In the zebrafish lateral line system, interneuromast cells (INCs) between neuromasts are kept quiescent by underlying Schwann cells (SWCs). Upon severe injuries that cause the complete loss of an entire neuromast, INCs can occasionally differentiate into neuromasts but how they escape from the inhibition by SWCs is still unclear. Using a genetic/chemical method to ablate a neuromast precisely, we found that a small portion of larvae can regenerate a new neuromast. However, the residual regeneration capacity was hindered by inhibiting macrophages. Using in toto imaging, we further discovered heterogeneities in macrophage behavior and distribution along the lateral line. We witnessed the crawling of macrophages between the injured lateral line and SWCs during regeneration and between the second primordium and the first mature lateral line during development. It implies that macrophages may physically alleviate the nerve inhibition to break the dormancy of INCs during regeneration and development in the zebrafish lateral line.https://www.frontiersin.org/articles/10.3389/fcell.2022.907863/fullregenerationlateral lineinterneuromast cellSchwann cellWntmacrophage |
| spellingShingle | Meng-Ju Lin Chia-Ming Lee Wei-Lin Hsu Bi-Chang Chen Shyh-Jye Lee Shyh-Jye Lee Shyh-Jye Lee Macrophages Break Interneuromast Cell Quiescence by Intervening in the Inhibition of Schwann Cells in the Zebrafish Lateral Line Frontiers in Cell and Developmental Biology regeneration lateral line interneuromast cell Schwann cell Wnt macrophage |
| title | Macrophages Break Interneuromast Cell Quiescence by Intervening in the Inhibition of Schwann Cells in the Zebrafish Lateral Line |
| title_full | Macrophages Break Interneuromast Cell Quiescence by Intervening in the Inhibition of Schwann Cells in the Zebrafish Lateral Line |
| title_fullStr | Macrophages Break Interneuromast Cell Quiescence by Intervening in the Inhibition of Schwann Cells in the Zebrafish Lateral Line |
| title_full_unstemmed | Macrophages Break Interneuromast Cell Quiescence by Intervening in the Inhibition of Schwann Cells in the Zebrafish Lateral Line |
| title_short | Macrophages Break Interneuromast Cell Quiescence by Intervening in the Inhibition of Schwann Cells in the Zebrafish Lateral Line |
| title_sort | macrophages break interneuromast cell quiescence by intervening in the inhibition of schwann cells in the zebrafish lateral line |
| topic | regeneration lateral line interneuromast cell Schwann cell Wnt macrophage |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2022.907863/full |
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