Extraction, phytochemical characterization, and antifungal activity of Salvia rosmarinus extract
In the current study, we used high-performance liquid chromatography (HPLC) and gas chromatography–mass spectrometry (GC–MS) to investigate and analyze the methanolic extract of Salvia rosmarinus leaves. HPLC analysis showed that the extract revealed a diverse array of polyphenolic compounds, includ...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2023-09-01
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| Series: | Open Chemistry |
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| Online Access: | https://doi.org/10.1515/chem-2023-0124 |
| _version_ | 1797668778195025920 |
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| author | Aamer Helmy A. Al-Askar Abdulaziz A. Gaber Mahmoud A. El-Tanbouly Rania Abdelkhalek Ahmed Behiry Said Elsharkawy Mohsen M. Kowalczewski Przemysław Łukasz El-Messeiry Sarah |
| author_facet | Aamer Helmy A. Al-Askar Abdulaziz A. Gaber Mahmoud A. El-Tanbouly Rania Abdelkhalek Ahmed Behiry Said Elsharkawy Mohsen M. Kowalczewski Przemysław Łukasz El-Messeiry Sarah |
| author_sort | Aamer Helmy A. |
| collection | DOAJ |
| description | In the current study, we used high-performance liquid chromatography (HPLC) and gas chromatography–mass spectrometry (GC–MS) to investigate and analyze the methanolic extract of Salvia rosmarinus leaves. HPLC analysis showed that the extract revealed a diverse array of polyphenolic compounds, including apigenin, catechin, chlorogenic acid, cinnamic acid, caffeic acid, coumaric acid, daidzein, ellagic acid, ferulic acid, gallic acid, hesperetin, kaempferol, methyl gallate, naringenin, pyrocatechol, quercetin, rutin, syringic acid, and vanillin. Furthermore, three fungal isolates from symptomatic strawberry plants were obtained and identified as Botrytis cinerea (OR116486), Fusarium oxysporum (OR116505), and Rhizoctonia solani (OR116525). The extract’s antifungal activity was evaluated at concentrations of 0, 50, 150, 200, and 300 µg/mL. At 200 µg/mL, the extract showed growth inhibition percentages of 74.56, 58.19, and 56.67% for R. solani, F. oxysporum, and B. cinerea, respectively, while at 300 µg/mL, all the tested fungi were completely suppressed. The GC–MS analysis revealed that the major compounds of the methanolic extract identified based on their retention times and relative peak areas (%) included β-caryophyllene (12.06%), germacrene d (13.55%), caryophyllene oxide (3.13%), methyl palmitate (5.26%), hexadecanoic acid (4.9%), and methyl stearate (6.02%). These results show rosemary extract’s potential as a source of natural antifungal agents against plant photogenic fungi. As a result, it provides a safer alternative to the current protective approaches for plant disease management. |
| first_indexed | 2024-03-11T20:34:27Z |
| format | Article |
| id | doaj.art-8c6729f083e94ea88465d85ea1e2b10a |
| institution | Directory Open Access Journal |
| issn | 2391-5420 |
| language | English |
| last_indexed | 2024-03-11T20:34:27Z |
| publishDate | 2023-09-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Chemistry |
| spelling | doaj.art-8c6729f083e94ea88465d85ea1e2b10a2023-10-02T07:37:43ZengDe GruyterOpen Chemistry2391-54202023-09-012116519710.1515/chem-2023-0124Extraction, phytochemical characterization, and antifungal activity of Salvia rosmarinus extractAamer Helmy A.0Al-Askar Abdulaziz A.1Gaber Mahmoud A.2El-Tanbouly Rania3Abdelkhalek Ahmed4Behiry Said5Elsharkawy Mohsen M.6Kowalczewski Przemysław Łukasz7El-Messeiry Sarah8Department of Chemistry and Technology of Pesticide, Agriculture Faculty (El-Shatby), Alexandria University, Alexandria21545, EgyptDepartment of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Plant Pathology, Faculty of Agriculture (El-Shatby), Alexandria University, Alexandria21545, EgyptDepartment of Floriculture, Ornamental Horticulture and Landscape Design, Faculty of Agriculture (El-Shatby), Alexandria University, Alexandria21545, EgyptPlant Protection and Biomolecular Diagnosis Department, Arid Lands Cultivation Research Institute, City of Scientific Research and Technological Applications, Alexandria21934, EgyptAgricultural Botany Department, Faculty of Agriculture (Saba Basha), Alexandria University, Alexandria21531, EgyptDepartment of Agricultural Botany, Faculty of Agriculture, Kafrelsheikh University, Kafrelsheikh33516, EgyptDepartment of Food Technology of Plant Origin, Poznań University of Life Sciences, Poznań, PolandDepartment of Genetics, Faculty of Agriculture (El-Shatby), Alexandria University, Alexandria21545, EgyptIn the current study, we used high-performance liquid chromatography (HPLC) and gas chromatography–mass spectrometry (GC–MS) to investigate and analyze the methanolic extract of Salvia rosmarinus leaves. HPLC analysis showed that the extract revealed a diverse array of polyphenolic compounds, including apigenin, catechin, chlorogenic acid, cinnamic acid, caffeic acid, coumaric acid, daidzein, ellagic acid, ferulic acid, gallic acid, hesperetin, kaempferol, methyl gallate, naringenin, pyrocatechol, quercetin, rutin, syringic acid, and vanillin. Furthermore, three fungal isolates from symptomatic strawberry plants were obtained and identified as Botrytis cinerea (OR116486), Fusarium oxysporum (OR116505), and Rhizoctonia solani (OR116525). The extract’s antifungal activity was evaluated at concentrations of 0, 50, 150, 200, and 300 µg/mL. At 200 µg/mL, the extract showed growth inhibition percentages of 74.56, 58.19, and 56.67% for R. solani, F. oxysporum, and B. cinerea, respectively, while at 300 µg/mL, all the tested fungi were completely suppressed. The GC–MS analysis revealed that the major compounds of the methanolic extract identified based on their retention times and relative peak areas (%) included β-caryophyllene (12.06%), germacrene d (13.55%), caryophyllene oxide (3.13%), methyl palmitate (5.26%), hexadecanoic acid (4.9%), and methyl stearate (6.02%). These results show rosemary extract’s potential as a source of natural antifungal agents against plant photogenic fungi. As a result, it provides a safer alternative to the current protective approaches for plant disease management.https://doi.org/10.1515/chem-2023-0124salvia rosmarinusmethanolic extracthplcgc-msantifungal |
| spellingShingle | Aamer Helmy A. Al-Askar Abdulaziz A. Gaber Mahmoud A. El-Tanbouly Rania Abdelkhalek Ahmed Behiry Said Elsharkawy Mohsen M. Kowalczewski Przemysław Łukasz El-Messeiry Sarah Extraction, phytochemical characterization, and antifungal activity of Salvia rosmarinus extract Open Chemistry salvia rosmarinus methanolic extract hplc gc-ms antifungal |
| title | Extraction, phytochemical characterization, and antifungal activity of Salvia rosmarinus extract |
| title_full | Extraction, phytochemical characterization, and antifungal activity of Salvia rosmarinus extract |
| title_fullStr | Extraction, phytochemical characterization, and antifungal activity of Salvia rosmarinus extract |
| title_full_unstemmed | Extraction, phytochemical characterization, and antifungal activity of Salvia rosmarinus extract |
| title_short | Extraction, phytochemical characterization, and antifungal activity of Salvia rosmarinus extract |
| title_sort | extraction phytochemical characterization and antifungal activity of salvia rosmarinus extract |
| topic | salvia rosmarinus methanolic extract hplc gc-ms antifungal |
| url | https://doi.org/10.1515/chem-2023-0124 |
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