Regulation mechanisms of flavonoids biosynthesis of Hancheng Dahongpao peels (Zanthoxylum bungeanum Maxim) at different development stages by integrated metabolomics and transcriptomics analysis
Abstract Background Flavonoids have strong free radical scavenging and antioxidant capacity. The high abundance of flavonoids in Chinese prickly ash peels have many benefits to human health. In this study, ‘Hancheng Dahongpao’, a main cultivar, was taken as materials to investigate the flavonoids bi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-05-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-022-03642-5 |
| _version_ | 1811341055520407552 |
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| author | Tao Zheng Jun Han Ke-xing Su Bing-yin Sun Shu-ming Liu |
| author_facet | Tao Zheng Jun Han Ke-xing Su Bing-yin Sun Shu-ming Liu |
| author_sort | Tao Zheng |
| collection | DOAJ |
| description | Abstract Background Flavonoids have strong free radical scavenging and antioxidant capacity. The high abundance of flavonoids in Chinese prickly ash peels have many benefits to human health. In this study, ‘Hancheng Dahongpao’, a main cultivar, was taken as materials to investigate the flavonoids biosynthesis mechanism of Zanthoxylum bungeanum Maxim at three key development stages by integration of metabolomics and transcriptomics analysis. Results A total of 19 differentially accumulated metabolites were identified, the key flavonoids compounds were kaempferol, quercetin and their glycoside derivatives, and two major anthocyanins (peonidin O-hexoside and peonidin 3-O-glucoside). 5 gene networks/modules including 15 important candidate genes were identified, which was highly correlated with flavonoids. Among these genes, ZM-163828 and ZM-184209 were strongly correlated with kaempferol and quercetin, and ZM-125833 and ZM-97481 were controlled the anthocyanins biosynthesis. Moreover, it was shown that MYB-ZM1, MYB-ZM3, MYB-ZM5, MYB-ZM6 and MYB-ZM7 coordinately controlled flavonoids accumulation through regulating the structural genes. Conclusions Generally, this study systematically revealed the flavonoids metabolic pathways and candidate genes involved in flavonoids biosynthesis and laid a foundation for the potential targets for the breeding of new valuable Chinese prickly ash cultivars. |
| first_indexed | 2024-04-13T18:51:54Z |
| format | Article |
| id | doaj.art-8c6873373c584e02ae8445c10304b210 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-13T18:51:54Z |
| publishDate | 2022-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-8c6873373c584e02ae8445c10304b2102022-12-22T02:34:24ZengBMCBMC Plant Biology1471-22292022-05-0122111510.1186/s12870-022-03642-5Regulation mechanisms of flavonoids biosynthesis of Hancheng Dahongpao peels (Zanthoxylum bungeanum Maxim) at different development stages by integrated metabolomics and transcriptomics analysisTao Zheng0Jun Han1Ke-xing Su2Bing-yin Sun3Shu-ming Liu4Northwest Agriculture and Forestry University, College of ScienceForestry and Grassland Bureau of Xunhua Salar autonomous countyNorthwest Agriculture and Forestry University, College of ScienceYangling Vocational &Technical CollegeNorthwest Agriculture and Forestry University, College of ScienceAbstract Background Flavonoids have strong free radical scavenging and antioxidant capacity. The high abundance of flavonoids in Chinese prickly ash peels have many benefits to human health. In this study, ‘Hancheng Dahongpao’, a main cultivar, was taken as materials to investigate the flavonoids biosynthesis mechanism of Zanthoxylum bungeanum Maxim at three key development stages by integration of metabolomics and transcriptomics analysis. Results A total of 19 differentially accumulated metabolites were identified, the key flavonoids compounds were kaempferol, quercetin and their glycoside derivatives, and two major anthocyanins (peonidin O-hexoside and peonidin 3-O-glucoside). 5 gene networks/modules including 15 important candidate genes were identified, which was highly correlated with flavonoids. Among these genes, ZM-163828 and ZM-184209 were strongly correlated with kaempferol and quercetin, and ZM-125833 and ZM-97481 were controlled the anthocyanins biosynthesis. Moreover, it was shown that MYB-ZM1, MYB-ZM3, MYB-ZM5, MYB-ZM6 and MYB-ZM7 coordinately controlled flavonoids accumulation through regulating the structural genes. Conclusions Generally, this study systematically revealed the flavonoids metabolic pathways and candidate genes involved in flavonoids biosynthesis and laid a foundation for the potential targets for the breeding of new valuable Chinese prickly ash cultivars.https://doi.org/10.1186/s12870-022-03642-5Zanthoxylum bungeanum Maxim ‘Hancheng Dahongpao’TranscriptomeMetabolomeFlavonoids biosynthesis |
| spellingShingle | Tao Zheng Jun Han Ke-xing Su Bing-yin Sun Shu-ming Liu Regulation mechanisms of flavonoids biosynthesis of Hancheng Dahongpao peels (Zanthoxylum bungeanum Maxim) at different development stages by integrated metabolomics and transcriptomics analysis BMC Plant Biology Zanthoxylum bungeanum Maxim ‘Hancheng Dahongpao’ Transcriptome Metabolome Flavonoids biosynthesis |
| title | Regulation mechanisms of flavonoids biosynthesis of Hancheng Dahongpao peels (Zanthoxylum bungeanum Maxim) at different development stages by integrated metabolomics and transcriptomics analysis |
| title_full | Regulation mechanisms of flavonoids biosynthesis of Hancheng Dahongpao peels (Zanthoxylum bungeanum Maxim) at different development stages by integrated metabolomics and transcriptomics analysis |
| title_fullStr | Regulation mechanisms of flavonoids biosynthesis of Hancheng Dahongpao peels (Zanthoxylum bungeanum Maxim) at different development stages by integrated metabolomics and transcriptomics analysis |
| title_full_unstemmed | Regulation mechanisms of flavonoids biosynthesis of Hancheng Dahongpao peels (Zanthoxylum bungeanum Maxim) at different development stages by integrated metabolomics and transcriptomics analysis |
| title_short | Regulation mechanisms of flavonoids biosynthesis of Hancheng Dahongpao peels (Zanthoxylum bungeanum Maxim) at different development stages by integrated metabolomics and transcriptomics analysis |
| title_sort | regulation mechanisms of flavonoids biosynthesis of hancheng dahongpao peels zanthoxylum bungeanum maxim at different development stages by integrated metabolomics and transcriptomics analysis |
| topic | Zanthoxylum bungeanum Maxim ‘Hancheng Dahongpao’ Transcriptome Metabolome Flavonoids biosynthesis |
| url | https://doi.org/10.1186/s12870-022-03642-5 |
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