Gut Microbiome Signatures in the Progression of Hepatitis B Virus-Induced Liver Disease
The gut microbiome is associated with hepatitis B virus (HBV)-induced liver disease, which progresses from chronic hepatitis B, to liver cirrhosis, and eventually to hepatocellular carcinoma. Studies have analyzed the gut microbiome at each stage of HBV-induced liver diseases, but a consensus has no...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.916061/full |
| _version_ | 1811246172376924160 |
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| author | Ranxi Li Ranxi Li Xinzhu Yi Junhao Yang Zhou Zhu Zhou Zhu Yifei Wang Yifei Wang Xiaomin Liu Xili Huang Xili Huang Yu Wan Xihua Fu Wensheng Shu Wenjie Zhang Zhang Wang Zhang Wang |
| author_facet | Ranxi Li Ranxi Li Xinzhu Yi Junhao Yang Zhou Zhu Zhou Zhu Yifei Wang Yifei Wang Xiaomin Liu Xili Huang Xili Huang Yu Wan Xihua Fu Wensheng Shu Wenjie Zhang Zhang Wang Zhang Wang |
| author_sort | Ranxi Li |
| collection | DOAJ |
| description | The gut microbiome is associated with hepatitis B virus (HBV)-induced liver disease, which progresses from chronic hepatitis B, to liver cirrhosis, and eventually to hepatocellular carcinoma. Studies have analyzed the gut microbiome at each stage of HBV-induced liver diseases, but a consensus has not been reached on the microbial signatures across these stages. Here, we conducted by a systematic meta-analysis of 486 fecal samples from publicly available 16S rRNA gene datasets across all disease stages, and validated the results by a gut microbiome characterization on an independent cohort of 15 controls, 23 chronic hepatitis B, 20 liver cirrhosis, and 22 hepatocellular carcinoma patients. The integrative analyses revealed 13 genera consistently altered at each of the disease stages both in public and validation datasets, suggesting highly robust microbiome signatures. Specifically, Colidextribacter and Monoglobus were enriched in healthy controls. An unclassified Lachnospiraceae genus was specifically elevated in chronic hepatitis B, whereas Bilophia was depleted. Prevotella and Oscillibacter were depleted in liver cirrhosis. And Coprococcus and Faecalibacterium were depleted in hepatocellular carcinoma. Classifiers established using these 13 genera showed diagnostic power across all disease stages in a cross-validation between public and validation datasets (AUC = 0.65–0.832). The identified microbial taxonomy serves as non-invasive biomarkers for monitoring the progression of HBV-induced liver disease, and may contribute to microbiome-based therapies. |
| first_indexed | 2024-04-12T14:49:52Z |
| format | Article |
| id | doaj.art-8c68cdc0c33840b6a1914c18e8f5c463 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T14:49:52Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-8c68cdc0c33840b6a1914c18e8f5c4632022-12-22T03:28:31ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-06-011310.3389/fmicb.2022.916061916061Gut Microbiome Signatures in the Progression of Hepatitis B Virus-Induced Liver DiseaseRanxi Li0Ranxi Li1Xinzhu Yi2Junhao Yang3Zhou Zhu4Zhou Zhu5Yifei Wang6Yifei Wang7Xiaomin Liu8Xili Huang9Xili Huang10Yu Wan11Xihua Fu12Wensheng Shu13Wenjie Zhang14Zhang Wang15Zhang Wang16South China Normal University-Panyu Central Hospital Joint Laboratory of Basic and Translational Medical Research, Guangzhou Panyu Central Hospital, Guangzhou, ChinaSchool of Life Sciences, South China Normal University, Guangzhou, ChinaSchool of Life Sciences, South China Normal University, Guangzhou, ChinaSchool of Life Sciences, South China Normal University, Guangzhou, ChinaSouth China Normal University-Panyu Central Hospital Joint Laboratory of Basic and Translational Medical Research, Guangzhou Panyu Central Hospital, Guangzhou, ChinaSchool of Life Sciences, South China Normal University, Guangzhou, ChinaSouth China Normal University-Panyu Central Hospital Joint Laboratory of Basic and Translational Medical Research, Guangzhou Panyu Central Hospital, Guangzhou, ChinaSchool of Life Sciences, South China Normal University, Guangzhou, ChinaSchool of Life Sciences, South China Normal University, Guangzhou, ChinaSouth China Normal University-Panyu Central Hospital Joint Laboratory of Basic and Translational Medical Research, Guangzhou Panyu Central Hospital, Guangzhou, ChinaSchool of Life Sciences, South China Normal University, Guangzhou, ChinaDepartment of Gastroenterology, Guangzhou Panyu Central Hospital, Guangzhou, ChinaDepartment of Infectious Diseases, Guangzhou Panyu Central Hospital, Guangzhou, ChinaSchool of Life Sciences, South China Normal University, Guangzhou, ChinaDepartment of Science and Education, Guangzhou Panyu Central Hospital, Guangzhou, ChinaSouth China Normal University-Panyu Central Hospital Joint Laboratory of Basic and Translational Medical Research, Guangzhou Panyu Central Hospital, Guangzhou, ChinaSchool of Life Sciences, South China Normal University, Guangzhou, ChinaThe gut microbiome is associated with hepatitis B virus (HBV)-induced liver disease, which progresses from chronic hepatitis B, to liver cirrhosis, and eventually to hepatocellular carcinoma. Studies have analyzed the gut microbiome at each stage of HBV-induced liver diseases, but a consensus has not been reached on the microbial signatures across these stages. Here, we conducted by a systematic meta-analysis of 486 fecal samples from publicly available 16S rRNA gene datasets across all disease stages, and validated the results by a gut microbiome characterization on an independent cohort of 15 controls, 23 chronic hepatitis B, 20 liver cirrhosis, and 22 hepatocellular carcinoma patients. The integrative analyses revealed 13 genera consistently altered at each of the disease stages both in public and validation datasets, suggesting highly robust microbiome signatures. Specifically, Colidextribacter and Monoglobus were enriched in healthy controls. An unclassified Lachnospiraceae genus was specifically elevated in chronic hepatitis B, whereas Bilophia was depleted. Prevotella and Oscillibacter were depleted in liver cirrhosis. And Coprococcus and Faecalibacterium were depleted in hepatocellular carcinoma. Classifiers established using these 13 genera showed diagnostic power across all disease stages in a cross-validation between public and validation datasets (AUC = 0.65–0.832). The identified microbial taxonomy serves as non-invasive biomarkers for monitoring the progression of HBV-induced liver disease, and may contribute to microbiome-based therapies.https://www.frontiersin.org/articles/10.3389/fmicb.2022.916061/fullhepatitisliver cirrhosishepatocellular carcinomahepatitis B virusmeta-analysisgut microbiome |
| spellingShingle | Ranxi Li Ranxi Li Xinzhu Yi Junhao Yang Zhou Zhu Zhou Zhu Yifei Wang Yifei Wang Xiaomin Liu Xili Huang Xili Huang Yu Wan Xihua Fu Wensheng Shu Wenjie Zhang Zhang Wang Zhang Wang Gut Microbiome Signatures in the Progression of Hepatitis B Virus-Induced Liver Disease Frontiers in Microbiology hepatitis liver cirrhosis hepatocellular carcinoma hepatitis B virus meta-analysis gut microbiome |
| title | Gut Microbiome Signatures in the Progression of Hepatitis B Virus-Induced Liver Disease |
| title_full | Gut Microbiome Signatures in the Progression of Hepatitis B Virus-Induced Liver Disease |
| title_fullStr | Gut Microbiome Signatures in the Progression of Hepatitis B Virus-Induced Liver Disease |
| title_full_unstemmed | Gut Microbiome Signatures in the Progression of Hepatitis B Virus-Induced Liver Disease |
| title_short | Gut Microbiome Signatures in the Progression of Hepatitis B Virus-Induced Liver Disease |
| title_sort | gut microbiome signatures in the progression of hepatitis b virus induced liver disease |
| topic | hepatitis liver cirrhosis hepatocellular carcinoma hepatitis B virus meta-analysis gut microbiome |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.916061/full |
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