BEX1 is a critical determinant of viral myocarditis.
Viral infection of the heart is a common but underappreciated cause of heart failure. Viruses can cause direct cardiac damage by lysing infected cardiomyocytes. Inflammatory immune responses that limit viral replication can also indirectly cause damage during infection, making regulatory factors tha...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-02-01
|
| Series: | PLoS Pathogens |
| Online Access: | https://doi.org/10.1371/journal.ppat.1010342 |
| _version_ | 1797862115337306112 |
|---|---|
| author | Colton R Martens Lisa E Dorn Adam D Kenney Shyam S Bansal Jacob S Yount Federica Accornero |
| author_facet | Colton R Martens Lisa E Dorn Adam D Kenney Shyam S Bansal Jacob S Yount Federica Accornero |
| author_sort | Colton R Martens |
| collection | DOAJ |
| description | Viral infection of the heart is a common but underappreciated cause of heart failure. Viruses can cause direct cardiac damage by lysing infected cardiomyocytes. Inflammatory immune responses that limit viral replication can also indirectly cause damage during infection, making regulatory factors that fine-tune these responses particularly important. Identifying and understanding these factors that regulate cardiac immune responses during infection will be essential for developing targeted treatments for virus-associated heart failure. Our laboratory has discovered Brain Expressed X-linked protein 1 (BEX1) as a novel stress-regulated pro-inflammatory factor in the heart. Here we report that BEX1 plays a cardioprotective role in the heart during viral infection. Specifically, we adopted genetic gain- and loss-of-function strategies to modulate BEX1 expression in the heart in the context of coxsackievirus B3 (CVB3)-induced cardiomyopathy and found that BEX1 limits viral replication in cardiomyocytes. Interestingly, despite the greater viral load observed in mice lacking BEX1, inflammatory immune cell recruitment in the mouse heart was profoundly impaired in the absence of BEX1. Overall, the absence of BEX1 accelerated CVB3-driven heart failure and pathologic heart remodeling. This result suggests that limiting inflammatory cell recruitment has detrimental consequences for the heart during viral infections. Conversely, transgenic mice overexpressing BEX1 in cardiomyocytes revealed the efficacy of BEX1 for counteracting viral replication in the heart in vivo. We also found that BEX1 retains its antiviral role in isolated cells. Indeed, BEX1 was necessary and sufficient to counteract viral replication in both isolated primary cardiomyocytes and mouse embryonic fibroblasts suggesting a broader applicability of BEX1 as antiviral agent that extended to viruses other than CVB3, including Influenza A and Sendai virus. Mechanistically, BEX1 regulated interferon beta (IFN-β) expression in infected cells. Overall, our study suggests a multifaceted role of BEX1 in the cardiac antiviral immune response. |
| first_indexed | 2024-04-09T22:14:09Z |
| format | Article |
| id | doaj.art-8c6a49a89578411b9e205be4566e928a |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-04-09T22:14:09Z |
| publishDate | 2022-02-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-8c6a49a89578411b9e205be4566e928a2023-03-23T05:31:28ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742022-02-01182e101034210.1371/journal.ppat.1010342BEX1 is a critical determinant of viral myocarditis.Colton R MartensLisa E DornAdam D KenneyShyam S BansalJacob S YountFederica AccorneroViral infection of the heart is a common but underappreciated cause of heart failure. Viruses can cause direct cardiac damage by lysing infected cardiomyocytes. Inflammatory immune responses that limit viral replication can also indirectly cause damage during infection, making regulatory factors that fine-tune these responses particularly important. Identifying and understanding these factors that regulate cardiac immune responses during infection will be essential for developing targeted treatments for virus-associated heart failure. Our laboratory has discovered Brain Expressed X-linked protein 1 (BEX1) as a novel stress-regulated pro-inflammatory factor in the heart. Here we report that BEX1 plays a cardioprotective role in the heart during viral infection. Specifically, we adopted genetic gain- and loss-of-function strategies to modulate BEX1 expression in the heart in the context of coxsackievirus B3 (CVB3)-induced cardiomyopathy and found that BEX1 limits viral replication in cardiomyocytes. Interestingly, despite the greater viral load observed in mice lacking BEX1, inflammatory immune cell recruitment in the mouse heart was profoundly impaired in the absence of BEX1. Overall, the absence of BEX1 accelerated CVB3-driven heart failure and pathologic heart remodeling. This result suggests that limiting inflammatory cell recruitment has detrimental consequences for the heart during viral infections. Conversely, transgenic mice overexpressing BEX1 in cardiomyocytes revealed the efficacy of BEX1 for counteracting viral replication in the heart in vivo. We also found that BEX1 retains its antiviral role in isolated cells. Indeed, BEX1 was necessary and sufficient to counteract viral replication in both isolated primary cardiomyocytes and mouse embryonic fibroblasts suggesting a broader applicability of BEX1 as antiviral agent that extended to viruses other than CVB3, including Influenza A and Sendai virus. Mechanistically, BEX1 regulated interferon beta (IFN-β) expression in infected cells. Overall, our study suggests a multifaceted role of BEX1 in the cardiac antiviral immune response.https://doi.org/10.1371/journal.ppat.1010342 |
| spellingShingle | Colton R Martens Lisa E Dorn Adam D Kenney Shyam S Bansal Jacob S Yount Federica Accornero BEX1 is a critical determinant of viral myocarditis. PLoS Pathogens |
| title | BEX1 is a critical determinant of viral myocarditis. |
| title_full | BEX1 is a critical determinant of viral myocarditis. |
| title_fullStr | BEX1 is a critical determinant of viral myocarditis. |
| title_full_unstemmed | BEX1 is a critical determinant of viral myocarditis. |
| title_short | BEX1 is a critical determinant of viral myocarditis. |
| title_sort | bex1 is a critical determinant of viral myocarditis |
| url | https://doi.org/10.1371/journal.ppat.1010342 |
| work_keys_str_mv | AT coltonrmartens bex1isacriticaldeterminantofviralmyocarditis AT lisaedorn bex1isacriticaldeterminantofviralmyocarditis AT adamdkenney bex1isacriticaldeterminantofviralmyocarditis AT shyamsbansal bex1isacriticaldeterminantofviralmyocarditis AT jacobsyount bex1isacriticaldeterminantofviralmyocarditis AT federicaaccornero bex1isacriticaldeterminantofviralmyocarditis |