Genome-Wide Identification of the 1-Aminocyclopropane-1-carboxylic Acid Synthase (<i>ACS</i>) Genes and Their Possible Role in Sand Pear (<i>Pyrus pyrifolia</i>) Fruit Ripening
Ethylene production is negatively associated with storage life in sand pear (<i>Pyrus pyrifolia</i> Nakai), particularly at the time of fruit harvest. 1-Aminocyclopropane-1-carboxylic acid synthase (ACS) is the rate-limiting enzyme in ethylene biosynthesis and is considered to be importa...
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MDPI AG
2021-10-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/7/10/401 |
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| author | Jing-Guo Zhang Wei Du Jing Fan Xiao-Ping Yang Qi-Liang Chen Ying Liu Hong-Ju Hu Zheng-Rong Luo |
| author_facet | Jing-Guo Zhang Wei Du Jing Fan Xiao-Ping Yang Qi-Liang Chen Ying Liu Hong-Ju Hu Zheng-Rong Luo |
| author_sort | Jing-Guo Zhang |
| collection | DOAJ |
| description | Ethylene production is negatively associated with storage life in sand pear (<i>Pyrus pyrifolia</i> Nakai), particularly at the time of fruit harvest. 1-Aminocyclopropane-1-carboxylic acid synthase (ACS) is the rate-limiting enzyme in ethylene biosynthesis and is considered to be important for fruit storage life. However, the candidate <i>ACS</i> genes and their roles in sand pear remain unclear. The present study identified 13 <i>ACS</i> genes from the sand pear genome. Phylogenetic analysis categorized these ACS genes into four subgroups (type I, type II, type III and putative AAT), and indicated a close relationship between sand pear and Chinese white pear (<i>P. bretschneideri</i>). According to the RNA-seq data and qRT-PCR analysis, <i>PpyACS1</i>, <i>PpyACS2</i>, <i>PpyACS3</i>, <i>PpyACS8</i>, <i>PpyACS9</i>, <i>PpyACS12</i> and <i>PpyACS13</i> were differently expressed in climacteric and non-climacteric-type pear fruits, ‘Ninomiyahakuri’ and ‘Eli No.2’, respectively, during fruit ripening. In addition, the expressions of <i>PpyACS2</i>, <i>PpyACS8</i>, <i>PpyACS12</i> and <i>PpyACS13</i> were found to be associated with system 1 of ethylene production, while <i>PpyACS1</i>, <i>PpyACS3</i>, and <i>PpyACS9</i> were found to be associated with system 2, indicating that these <i>ACS</i> genes have different roles in ethylene biosynthesis during fruit development. Overall, our study provides fundamental knowledge on the characteristics of the <i>ACS</i> gene family in sand pear, in addition to their possible roles in fruit ripening. |
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| language | English |
| last_indexed | 2024-03-10T06:31:53Z |
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| spelling | doaj.art-8c6a861926e44ec19fb9d16f6e3e5e202023-11-22T18:27:45ZengMDPI AGHorticulturae2311-75242021-10-0171040110.3390/horticulturae7100401Genome-Wide Identification of the 1-Aminocyclopropane-1-carboxylic Acid Synthase (<i>ACS</i>) Genes and Their Possible Role in Sand Pear (<i>Pyrus pyrifolia</i>) Fruit RipeningJing-Guo Zhang0Wei Du1Jing Fan2Xiao-Ping Yang3Qi-Liang Chen4Ying Liu5Hong-Ju Hu6Zheng-Rong Luo7Key Laboratory of Horticultural Plant Biology, Huazhong Agricultural University, Wuhan 430070, ChinaResearch Institute of Fruit and Tea, Hubei Academy of Agricultural Science, Wuhan 430064, ChinaResearch Institute of Fruit and Tea, Hubei Academy of Agricultural Science, Wuhan 430064, ChinaResearch Institute of Fruit and Tea, Hubei Academy of Agricultural Science, Wuhan 430064, ChinaResearch Institute of Fruit and Tea, Hubei Academy of Agricultural Science, Wuhan 430064, ChinaKey Laboratory of Horticultural Plant Biology, Huazhong Agricultural University, Wuhan 430070, ChinaResearch Institute of Fruit and Tea, Hubei Academy of Agricultural Science, Wuhan 430064, ChinaKey Laboratory of Horticultural Plant Biology, Huazhong Agricultural University, Wuhan 430070, ChinaEthylene production is negatively associated with storage life in sand pear (<i>Pyrus pyrifolia</i> Nakai), particularly at the time of fruit harvest. 1-Aminocyclopropane-1-carboxylic acid synthase (ACS) is the rate-limiting enzyme in ethylene biosynthesis and is considered to be important for fruit storage life. However, the candidate <i>ACS</i> genes and their roles in sand pear remain unclear. The present study identified 13 <i>ACS</i> genes from the sand pear genome. Phylogenetic analysis categorized these ACS genes into four subgroups (type I, type II, type III and putative AAT), and indicated a close relationship between sand pear and Chinese white pear (<i>P. bretschneideri</i>). According to the RNA-seq data and qRT-PCR analysis, <i>PpyACS1</i>, <i>PpyACS2</i>, <i>PpyACS3</i>, <i>PpyACS8</i>, <i>PpyACS9</i>, <i>PpyACS12</i> and <i>PpyACS13</i> were differently expressed in climacteric and non-climacteric-type pear fruits, ‘Ninomiyahakuri’ and ‘Eli No.2’, respectively, during fruit ripening. In addition, the expressions of <i>PpyACS2</i>, <i>PpyACS8</i>, <i>PpyACS12</i> and <i>PpyACS13</i> were found to be associated with system 1 of ethylene production, while <i>PpyACS1</i>, <i>PpyACS3</i>, and <i>PpyACS9</i> were found to be associated with system 2, indicating that these <i>ACS</i> genes have different roles in ethylene biosynthesis during fruit development. Overall, our study provides fundamental knowledge on the characteristics of the <i>ACS</i> gene family in sand pear, in addition to their possible roles in fruit ripening.https://www.mdpi.com/2311-7524/7/10/401sand pearfruit ripeningACS gene familyethylene biosynthesisclimactericnon-climacteric |
| spellingShingle | Jing-Guo Zhang Wei Du Jing Fan Xiao-Ping Yang Qi-Liang Chen Ying Liu Hong-Ju Hu Zheng-Rong Luo Genome-Wide Identification of the 1-Aminocyclopropane-1-carboxylic Acid Synthase (<i>ACS</i>) Genes and Their Possible Role in Sand Pear (<i>Pyrus pyrifolia</i>) Fruit Ripening Horticulturae sand pear fruit ripening ACS gene family ethylene biosynthesis climacteric non-climacteric |
| title | Genome-Wide Identification of the 1-Aminocyclopropane-1-carboxylic Acid Synthase (<i>ACS</i>) Genes and Their Possible Role in Sand Pear (<i>Pyrus pyrifolia</i>) Fruit Ripening |
| title_full | Genome-Wide Identification of the 1-Aminocyclopropane-1-carboxylic Acid Synthase (<i>ACS</i>) Genes and Their Possible Role in Sand Pear (<i>Pyrus pyrifolia</i>) Fruit Ripening |
| title_fullStr | Genome-Wide Identification of the 1-Aminocyclopropane-1-carboxylic Acid Synthase (<i>ACS</i>) Genes and Their Possible Role in Sand Pear (<i>Pyrus pyrifolia</i>) Fruit Ripening |
| title_full_unstemmed | Genome-Wide Identification of the 1-Aminocyclopropane-1-carboxylic Acid Synthase (<i>ACS</i>) Genes and Their Possible Role in Sand Pear (<i>Pyrus pyrifolia</i>) Fruit Ripening |
| title_short | Genome-Wide Identification of the 1-Aminocyclopropane-1-carboxylic Acid Synthase (<i>ACS</i>) Genes and Their Possible Role in Sand Pear (<i>Pyrus pyrifolia</i>) Fruit Ripening |
| title_sort | genome wide identification of the 1 aminocyclopropane 1 carboxylic acid synthase i acs i genes and their possible role in sand pear i pyrus pyrifolia i fruit ripening |
| topic | sand pear fruit ripening ACS gene family ethylene biosynthesis climacteric non-climacteric |
| url | https://www.mdpi.com/2311-7524/7/10/401 |
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