Identification and determination of myriocin in Isaria cicadae and its allies by LTQ-Orbitrap-HRMS
A hybrid linear ion trap-quadrupole-Orbitrap high-resolution mass spectrometry (LTQ-Orbitrap-HRMS) was used to qualitatively and quantitatively analyse the myriocin in Isaria cicadae and its allies. The samples were prepared with 95% methanol for 30 min by ultrasonic-assisted extraction. The target...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2017-10-01
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| Series: | Mycology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/21501203.2017.1383319 |
| _version_ | 1818285620145946624 |
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| author | Wen-ming Cheng Qun-lin Zhang Ze-hua Wu Zhi-yong Zhang Yi-ru Miao Fan Peng Chun-ru Li |
| author_facet | Wen-ming Cheng Qun-lin Zhang Ze-hua Wu Zhi-yong Zhang Yi-ru Miao Fan Peng Chun-ru Li |
| author_sort | Wen-ming Cheng |
| collection | DOAJ |
| description | A hybrid linear ion trap-quadrupole-Orbitrap high-resolution mass spectrometry (LTQ-Orbitrap-HRMS) was used to qualitatively and quantitatively analyse the myriocin in Isaria cicadae and its allies. The samples were prepared with 95% methanol for 30 min by ultrasonic-assisted extraction. The target compound was purified by ODS solid-phase extraction (SPE) column. The enriched samples were identified by mass spectrometry. The results showed that the contents of myriocin in both wild and artificial Isaria cicadae were below the detection limit, while a strain of Ophiocordyceps longissima and Cordyceps cicadae Shing (Dujiaolong), both closely related to the Isaria cicadae, and its asexual mycelia are rich in myriocin. It suggests that it may be wrong to consider C. cicadae as I. cicadae’s teleomorph in Genbank or Mycobank in many published reports based on chemical classification, and the species rich in myriocin is probably not Isaria cicadae. |
| first_indexed | 2024-12-13T01:11:35Z |
| format | Article |
| id | doaj.art-8c6ac53b314d477daac5fbde603a7d35 |
| institution | Directory Open Access Journal |
| issn | 2150-1203 2150-1211 |
| language | English |
| last_indexed | 2024-12-13T01:11:35Z |
| publishDate | 2017-10-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Mycology |
| spelling | doaj.art-8c6ac53b314d477daac5fbde603a7d352022-12-22T00:04:26ZengTaylor & Francis GroupMycology2150-12032150-12112017-10-018428629210.1080/21501203.2017.13833191383319Identification and determination of myriocin in Isaria cicadae and its allies by LTQ-Orbitrap-HRMSWen-ming Cheng0Qun-lin Zhang1Ze-hua Wu2Zhi-yong Zhang3Yi-ru Miao4Fan Peng5Chun-ru Li6Anhui Medical UniversityAnhui Medical UniversityAnhui Medical UniversityAnhui Medical UniversityAnhui Medical UniversityAnhui Agricultural UniversityZhejiang BioAsia Institute of Life SciencesA hybrid linear ion trap-quadrupole-Orbitrap high-resolution mass spectrometry (LTQ-Orbitrap-HRMS) was used to qualitatively and quantitatively analyse the myriocin in Isaria cicadae and its allies. The samples were prepared with 95% methanol for 30 min by ultrasonic-assisted extraction. The target compound was purified by ODS solid-phase extraction (SPE) column. The enriched samples were identified by mass spectrometry. The results showed that the contents of myriocin in both wild and artificial Isaria cicadae were below the detection limit, while a strain of Ophiocordyceps longissima and Cordyceps cicadae Shing (Dujiaolong), both closely related to the Isaria cicadae, and its asexual mycelia are rich in myriocin. It suggests that it may be wrong to consider C. cicadae as I. cicadae’s teleomorph in Genbank or Mycobank in many published reports based on chemical classification, and the species rich in myriocin is probably not Isaria cicadae.http://dx.doi.org/10.1080/21501203.2017.1383319Isaria cicadaemyriocindeterminationLC-MSLTQ-Obitrap-HRMS |
| spellingShingle | Wen-ming Cheng Qun-lin Zhang Ze-hua Wu Zhi-yong Zhang Yi-ru Miao Fan Peng Chun-ru Li Identification and determination of myriocin in Isaria cicadae and its allies by LTQ-Orbitrap-HRMS Mycology Isaria cicadae myriocin determination LC-MS LTQ-Obitrap-HRMS |
| title | Identification and determination of myriocin in Isaria cicadae and its allies by LTQ-Orbitrap-HRMS |
| title_full | Identification and determination of myriocin in Isaria cicadae and its allies by LTQ-Orbitrap-HRMS |
| title_fullStr | Identification and determination of myriocin in Isaria cicadae and its allies by LTQ-Orbitrap-HRMS |
| title_full_unstemmed | Identification and determination of myriocin in Isaria cicadae and its allies by LTQ-Orbitrap-HRMS |
| title_short | Identification and determination of myriocin in Isaria cicadae and its allies by LTQ-Orbitrap-HRMS |
| title_sort | identification and determination of myriocin in isaria cicadae and its allies by ltq orbitrap hrms |
| topic | Isaria cicadae myriocin determination LC-MS LTQ-Obitrap-HRMS |
| url | http://dx.doi.org/10.1080/21501203.2017.1383319 |
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