Heterologous Production of Isopropanol Using Metabolically Engineered <i>Acetobacterium woodii</i> Strains
The depletion of fossil fuel resources and the CO<sub>2</sub> emissions coupled with petroleum-based industrial processes present a relevant issue for the whole of society. An alternative to the fossil-based production of chemicals is microbial fermentation using acetogens. Acetogenic ba...
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| Format: | Article |
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MDPI AG
2023-11-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/10/12/1381 |
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| author | Franziska Höfele Teresa Schoch Catarina Oberlies Peter Dürre |
| author_facet | Franziska Höfele Teresa Schoch Catarina Oberlies Peter Dürre |
| author_sort | Franziska Höfele |
| collection | DOAJ |
| description | The depletion of fossil fuel resources and the CO<sub>2</sub> emissions coupled with petroleum-based industrial processes present a relevant issue for the whole of society. An alternative to the fossil-based production of chemicals is microbial fermentation using acetogens. Acetogenic bacteria are able to metabolize CO or CO<sub>2</sub> (+H<sub>2</sub>) via the Wood–Ljungdahl pathway. As isopropanol is widely used in a variety of industrial branches, it is advantageous to find a fossil-independent production process. In this study, <i>Acetobacterium woodii</i> was employed to produce isopropanol via plasmid-based expression of the enzymes thiolase A, CoA-transferase, acetoacetate decarboxylase and secondary alcohol dehydrogenase. An examination of the enzymes originating from different organisms led to a maximum isopropanol production of 5.64 ± 1.08 mM using CO<sub>2</sub> + H<sub>2</sub> as the carbon and energy source. To this end, the genes <i>thlA</i> (encoding thiolase A) and <i>ctfA/ctfB</i> (encoding CoA-transferase) of <i>Clostridium scatologenes</i>, <i>adc</i> (encoding acetoacetate decarboxylase) originating from <i>C. acetobutylicum</i> and <i>sadH</i> (encoding secondary alcohol dehydrogenase) of <i>C. beijerinckii</i> DSM 6423 were employed. Since bottlenecks in the isopropanol production pathway are known, optimization of the strain was investigated, resulting in a 2.5-fold increase in isopropanol concentration. |
| first_indexed | 2024-03-08T20:59:50Z |
| format | Article |
| id | doaj.art-8c6c31f2904c4585909088a20ba79368 |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-08T20:59:50Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-8c6c31f2904c4585909088a20ba793682023-12-22T13:54:05ZengMDPI AGBioengineering2306-53542023-11-011012138110.3390/bioengineering10121381Heterologous Production of Isopropanol Using Metabolically Engineered <i>Acetobacterium woodii</i> StrainsFranziska Höfele0Teresa Schoch1Catarina Oberlies2Peter Dürre3Institute of Molecular Biology and Biotechnology of Prokaryotes, Ulm University, 89081 Ulm, GermanyInstitute of Microbiology and Biotechnology, Ulm University, 89081 Ulm, GermanyInstitute of Microbiology and Biotechnology, Ulm University, 89081 Ulm, GermanyInstitute of Microbiology and Biotechnology, Ulm University, 89081 Ulm, GermanyThe depletion of fossil fuel resources and the CO<sub>2</sub> emissions coupled with petroleum-based industrial processes present a relevant issue for the whole of society. An alternative to the fossil-based production of chemicals is microbial fermentation using acetogens. Acetogenic bacteria are able to metabolize CO or CO<sub>2</sub> (+H<sub>2</sub>) via the Wood–Ljungdahl pathway. As isopropanol is widely used in a variety of industrial branches, it is advantageous to find a fossil-independent production process. In this study, <i>Acetobacterium woodii</i> was employed to produce isopropanol via plasmid-based expression of the enzymes thiolase A, CoA-transferase, acetoacetate decarboxylase and secondary alcohol dehydrogenase. An examination of the enzymes originating from different organisms led to a maximum isopropanol production of 5.64 ± 1.08 mM using CO<sub>2</sub> + H<sub>2</sub> as the carbon and energy source. To this end, the genes <i>thlA</i> (encoding thiolase A) and <i>ctfA/ctfB</i> (encoding CoA-transferase) of <i>Clostridium scatologenes</i>, <i>adc</i> (encoding acetoacetate decarboxylase) originating from <i>C. acetobutylicum</i> and <i>sadH</i> (encoding secondary alcohol dehydrogenase) of <i>C. beijerinckii</i> DSM 6423 were employed. Since bottlenecks in the isopropanol production pathway are known, optimization of the strain was investigated, resulting in a 2.5-fold increase in isopropanol concentration.https://www.mdpi.com/2306-5354/10/12/1381acetogens<i>Acetobacterium woodii</i>anaerobic fermentationWood–Ljungdahl pathwaysolventsisopropanol |
| spellingShingle | Franziska Höfele Teresa Schoch Catarina Oberlies Peter Dürre Heterologous Production of Isopropanol Using Metabolically Engineered <i>Acetobacterium woodii</i> Strains Bioengineering acetogens <i>Acetobacterium woodii</i> anaerobic fermentation Wood–Ljungdahl pathway solvents isopropanol |
| title | Heterologous Production of Isopropanol Using Metabolically Engineered <i>Acetobacterium woodii</i> Strains |
| title_full | Heterologous Production of Isopropanol Using Metabolically Engineered <i>Acetobacterium woodii</i> Strains |
| title_fullStr | Heterologous Production of Isopropanol Using Metabolically Engineered <i>Acetobacterium woodii</i> Strains |
| title_full_unstemmed | Heterologous Production of Isopropanol Using Metabolically Engineered <i>Acetobacterium woodii</i> Strains |
| title_short | Heterologous Production of Isopropanol Using Metabolically Engineered <i>Acetobacterium woodii</i> Strains |
| title_sort | heterologous production of isopropanol using metabolically engineered i acetobacterium woodii i strains |
| topic | acetogens <i>Acetobacterium woodii</i> anaerobic fermentation Wood–Ljungdahl pathway solvents isopropanol |
| url | https://www.mdpi.com/2306-5354/10/12/1381 |
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