Molecular modelling of the HCMV IL-10 protein isoforms and analysis of their interaction with the human IL-10 receptor
The human cytomegalovirus (HCMV) UL111A gene encodes several homologs of the cellular interleukin 10 (cIL-10). Alternative splicing in the UL111A region produces two relatively well-characterized transcripts designated cmvIL-10 (isoform A) and LAcmvIL-10 (isoform B). The cmvIL-10 protein is the best...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9704672/?tool=EBI |
| _version_ | 1811207360592478208 |
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| author | Simone Queiroz Pantaleão Lívia de Moraes Bomediano Camillo Tainan Cerqueira Neves Isabela de Godoy Menezes Lucas Matheus Stangherlin Helena Beatriz de Carvalho Ruthner Batista Emma Poole Michael Nevels Eric Alisson Philot Ana Ligia Scott Maria Cristina Carlan da Silva |
| author_facet | Simone Queiroz Pantaleão Lívia de Moraes Bomediano Camillo Tainan Cerqueira Neves Isabela de Godoy Menezes Lucas Matheus Stangherlin Helena Beatriz de Carvalho Ruthner Batista Emma Poole Michael Nevels Eric Alisson Philot Ana Ligia Scott Maria Cristina Carlan da Silva |
| author_sort | Simone Queiroz Pantaleão |
| collection | DOAJ |
| description | The human cytomegalovirus (HCMV) UL111A gene encodes several homologs of the cellular interleukin 10 (cIL-10). Alternative splicing in the UL111A region produces two relatively well-characterized transcripts designated cmvIL-10 (isoform A) and LAcmvIL-10 (isoform B). The cmvIL-10 protein is the best characterized, both structurally and functionally, and has many immunosuppressive activities similar to cIL-10, while LAcmvIL-10 has more restricted biological activities. Alternative splicing also results in five less studied UL111A transcripts encoding additional proteins homologous to cIL-10 (isoforms C to G). These transcripts were identified during productive HCMV infection of MRC-5 cells with the high passage laboratory adapted AD169 strain, and the structure and properties of the corresponding proteins are largely unknown. Moreover, it is unclear whether these protein isoforms are able to bind the cellular IL-10 receptor and induce signalling. In the present study, we investigated the expression spectrum of UL111A transcripts in fully permissive MRC-5 cells and semi permissive U251 cells infected with the low passage HCMV strain TB40E. We identified a new spliced transcript (H) expressed during productive infection. Using computational methods, we carried out molecular modelling studies on the three-dimensional structures of the HCMV IL-10 proteins encoded by the transcripts detected in our work (cmvIL-10 (A), LAcmvIL-10 (B), E, F and H) and on their interaction with the human IL-10 receptor (IL-10R1). The modelling predicts clear differences between the isoform structures. Furthermore, the in silico simulations (molecular dynamics simulation and normal-mode analyses) allowed us to evaluate regions that contain potential receptor binding sites in each isoform. The analyses demonstrate that the complexes between the isoforms and IL-10R1 present different types of molecular interactions and consequently different affinities and stabilities. The knowledge about structure and expression of specific viral IL-10 isoforms has implications for understanding of their properties and role in HCMV immune evasion and pathogenesis. |
| first_indexed | 2024-04-12T04:03:00Z |
| format | Article |
| id | doaj.art-8c6ca49c2e664a11b52a5d103104bd14 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T04:03:00Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-8c6ca49c2e664a11b52a5d103104bd142022-12-22T03:48:41ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-011711Molecular modelling of the HCMV IL-10 protein isoforms and analysis of their interaction with the human IL-10 receptorSimone Queiroz PantaleãoLívia de Moraes Bomediano CamilloTainan Cerqueira NevesIsabela de Godoy MenezesLucas Matheus StangherlinHelena Beatriz de Carvalho Ruthner BatistaEmma PooleMichael NevelsEric Alisson PhilotAna Ligia ScottMaria Cristina Carlan da SilvaThe human cytomegalovirus (HCMV) UL111A gene encodes several homologs of the cellular interleukin 10 (cIL-10). Alternative splicing in the UL111A region produces two relatively well-characterized transcripts designated cmvIL-10 (isoform A) and LAcmvIL-10 (isoform B). The cmvIL-10 protein is the best characterized, both structurally and functionally, and has many immunosuppressive activities similar to cIL-10, while LAcmvIL-10 has more restricted biological activities. Alternative splicing also results in five less studied UL111A transcripts encoding additional proteins homologous to cIL-10 (isoforms C to G). These transcripts were identified during productive HCMV infection of MRC-5 cells with the high passage laboratory adapted AD169 strain, and the structure and properties of the corresponding proteins are largely unknown. Moreover, it is unclear whether these protein isoforms are able to bind the cellular IL-10 receptor and induce signalling. In the present study, we investigated the expression spectrum of UL111A transcripts in fully permissive MRC-5 cells and semi permissive U251 cells infected with the low passage HCMV strain TB40E. We identified a new spliced transcript (H) expressed during productive infection. Using computational methods, we carried out molecular modelling studies on the three-dimensional structures of the HCMV IL-10 proteins encoded by the transcripts detected in our work (cmvIL-10 (A), LAcmvIL-10 (B), E, F and H) and on their interaction with the human IL-10 receptor (IL-10R1). The modelling predicts clear differences between the isoform structures. Furthermore, the in silico simulations (molecular dynamics simulation and normal-mode analyses) allowed us to evaluate regions that contain potential receptor binding sites in each isoform. The analyses demonstrate that the complexes between the isoforms and IL-10R1 present different types of molecular interactions and consequently different affinities and stabilities. The knowledge about structure and expression of specific viral IL-10 isoforms has implications for understanding of their properties and role in HCMV immune evasion and pathogenesis.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9704672/?tool=EBI |
| spellingShingle | Simone Queiroz Pantaleão Lívia de Moraes Bomediano Camillo Tainan Cerqueira Neves Isabela de Godoy Menezes Lucas Matheus Stangherlin Helena Beatriz de Carvalho Ruthner Batista Emma Poole Michael Nevels Eric Alisson Philot Ana Ligia Scott Maria Cristina Carlan da Silva Molecular modelling of the HCMV IL-10 protein isoforms and analysis of their interaction with the human IL-10 receptor PLoS ONE |
| title | Molecular modelling of the HCMV IL-10 protein isoforms and analysis of their interaction with the human IL-10 receptor |
| title_full | Molecular modelling of the HCMV IL-10 protein isoforms and analysis of their interaction with the human IL-10 receptor |
| title_fullStr | Molecular modelling of the HCMV IL-10 protein isoforms and analysis of their interaction with the human IL-10 receptor |
| title_full_unstemmed | Molecular modelling of the HCMV IL-10 protein isoforms and analysis of their interaction with the human IL-10 receptor |
| title_short | Molecular modelling of the HCMV IL-10 protein isoforms and analysis of their interaction with the human IL-10 receptor |
| title_sort | molecular modelling of the hcmv il 10 protein isoforms and analysis of their interaction with the human il 10 receptor |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9704672/?tool=EBI |
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