Temperature Significantly Regulates Gene Expressions of Vertebrae Chondrocytes and Osteoblasts in Juvenile Golden Pompano (Trachinotus ovatus)
To elucidate the possible molecular reaction of vertebral fusion in juvenile golden pompano at high temperature, we examined the changes in growth, vertebral fusion incidence, histological differences and transcription levels of genes related to bone development in juvenile golden pompano at 27 and...
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Frontiers Media S.A.
2021-06-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2021.668522/full |
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| author | Mingyang Han Mingyang Han Mingyang Han Mingyang Han Zhengyi Fu Zhengyi Fu Zhengyi Fu Rui Yang Rui Yang Rui Yang Jian G. Qin Zhenhua Ma Zhenhua Ma Zhenhua Ma |
| author_facet | Mingyang Han Mingyang Han Mingyang Han Mingyang Han Zhengyi Fu Zhengyi Fu Zhengyi Fu Rui Yang Rui Yang Rui Yang Jian G. Qin Zhenhua Ma Zhenhua Ma Zhenhua Ma |
| author_sort | Mingyang Han |
| collection | DOAJ |
| description | To elucidate the possible molecular reaction of vertebral fusion in juvenile golden pompano at high temperature, we examined the changes in growth, vertebral fusion incidence, histological differences and transcription levels of genes related to bone development in juvenile golden pompano at 27 and 33°C for 30 days, respectively. The growth rate of juvenile fish was faster when the water temperature was 33°C, but the incidence of vertebral fusion was higher. Prolonged high water temperature reduced the osteogenic layer in the growth zone of the vertebral endplate and the elastic externa. The endplate growth areas of the fused vertebrae were transformed into cartilage tissue, which was then remodeled into bone. The intervertebral notochord tissue was transformed into bone and the intervertebral space disappeared. In normal phenotypes of vertebrae, short-term high temperature could promote the expression of genes related to cartilage differentiation and maturation, as well as genes related to osteoblastic differentiation. With the increase of culture time, the expression of genes related to cartilage and osteogenesis development was inhibited. In fused vertebrae, cartilage proliferation was enhanced, osteogenic differentiation was inhibited, and matrix mineralization may be enhanced. Genes associated with the development of chondrocytes and osteoblasts in the vertebrae of juvenile Trachinotus ovatus were significantly regulated by temperature and time. The results may contribute to further understanding of the occurrence of vertebral fusion at high temperature. |
| first_indexed | 2024-12-14T20:20:36Z |
| format | Article |
| id | doaj.art-8c6cabb01a3f4b50a5be1ffb1baa32d2 |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-12-14T20:20:36Z |
| publishDate | 2021-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-8c6cabb01a3f4b50a5be1ffb1baa32d22022-12-21T22:48:44ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452021-06-01810.3389/fmars.2021.668522668522Temperature Significantly Regulates Gene Expressions of Vertebrae Chondrocytes and Osteoblasts in Juvenile Golden Pompano (Trachinotus ovatus)Mingyang Han0Mingyang Han1Mingyang Han2Mingyang Han3Zhengyi Fu4Zhengyi Fu5Zhengyi Fu6Rui Yang7Rui Yang8Rui Yang9Jian G. Qin10Zhenhua Ma11Zhenhua Ma12Zhenhua Ma13College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, ChinaTropical Aquaculture Research and Development Center, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya, ChinaKey Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, Guangzhou, ChinaSanya Tropical Fisheries Research Institute, Sanya, ChinaTropical Aquaculture Research and Development Center, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya, ChinaKey Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, Guangzhou, ChinaSanya Tropical Fisheries Research Institute, Sanya, ChinaTropical Aquaculture Research and Development Center, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya, ChinaKey Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, Guangzhou, ChinaSanya Tropical Fisheries Research Institute, Sanya, ChinaCollege of Science and Engineering, Flinders University, Adelaide, SA, AustraliaTropical Aquaculture Research and Development Center, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya, ChinaKey Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, Guangzhou, ChinaSanya Tropical Fisheries Research Institute, Sanya, ChinaTo elucidate the possible molecular reaction of vertebral fusion in juvenile golden pompano at high temperature, we examined the changes in growth, vertebral fusion incidence, histological differences and transcription levels of genes related to bone development in juvenile golden pompano at 27 and 33°C for 30 days, respectively. The growth rate of juvenile fish was faster when the water temperature was 33°C, but the incidence of vertebral fusion was higher. Prolonged high water temperature reduced the osteogenic layer in the growth zone of the vertebral endplate and the elastic externa. The endplate growth areas of the fused vertebrae were transformed into cartilage tissue, which was then remodeled into bone. The intervertebral notochord tissue was transformed into bone and the intervertebral space disappeared. In normal phenotypes of vertebrae, short-term high temperature could promote the expression of genes related to cartilage differentiation and maturation, as well as genes related to osteoblastic differentiation. With the increase of culture time, the expression of genes related to cartilage and osteogenesis development was inhibited. In fused vertebrae, cartilage proliferation was enhanced, osteogenic differentiation was inhibited, and matrix mineralization may be enhanced. Genes associated with the development of chondrocytes and osteoblasts in the vertebrae of juvenile Trachinotus ovatus were significantly regulated by temperature and time. The results may contribute to further understanding of the occurrence of vertebral fusion at high temperature.https://www.frontiersin.org/articles/10.3389/fmars.2021.668522/fullhigh water temperaturehistopathologicmolecular characterizationgene expressionvertebral fusion |
| spellingShingle | Mingyang Han Mingyang Han Mingyang Han Mingyang Han Zhengyi Fu Zhengyi Fu Zhengyi Fu Rui Yang Rui Yang Rui Yang Jian G. Qin Zhenhua Ma Zhenhua Ma Zhenhua Ma Temperature Significantly Regulates Gene Expressions of Vertebrae Chondrocytes and Osteoblasts in Juvenile Golden Pompano (Trachinotus ovatus) Frontiers in Marine Science high water temperature histopathologic molecular characterization gene expression vertebral fusion |
| title | Temperature Significantly Regulates Gene Expressions of Vertebrae Chondrocytes and Osteoblasts in Juvenile Golden Pompano (Trachinotus ovatus) |
| title_full | Temperature Significantly Regulates Gene Expressions of Vertebrae Chondrocytes and Osteoblasts in Juvenile Golden Pompano (Trachinotus ovatus) |
| title_fullStr | Temperature Significantly Regulates Gene Expressions of Vertebrae Chondrocytes and Osteoblasts in Juvenile Golden Pompano (Trachinotus ovatus) |
| title_full_unstemmed | Temperature Significantly Regulates Gene Expressions of Vertebrae Chondrocytes and Osteoblasts in Juvenile Golden Pompano (Trachinotus ovatus) |
| title_short | Temperature Significantly Regulates Gene Expressions of Vertebrae Chondrocytes and Osteoblasts in Juvenile Golden Pompano (Trachinotus ovatus) |
| title_sort | temperature significantly regulates gene expressions of vertebrae chondrocytes and osteoblasts in juvenile golden pompano trachinotus ovatus |
| topic | high water temperature histopathologic molecular characterization gene expression vertebral fusion |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2021.668522/full |
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