The cAMP Receptor Protein (CRP) of <i>Vibrio mimicus</i> Regulates Its Bacterial Growth, Type II Secretion System, Flagellum Formation, Adhesion Genes, and Virulence
<i>Vibrio mimicus</i> is a serious pathogen in aquatic animals, resulting in significant economic losses. The cAMP receptor protein (CRP) often acts as a central regulator in highly pathogenic pathogens. <i>V. mimicus</i> SCCF01 is a highly pathogenic strain isolated from yel...
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2024-01-01
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| Online Access: | https://www.mdpi.com/2076-2615/14/3/437 |
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| author | Ziqi Tian Fei Xiang Kun Peng Zhenyang Qin Yang Feng Bowen Huang Ping Ouyang Xiaoli Huang Defang Chen Weimin Lai Yi Geng |
| author_facet | Ziqi Tian Fei Xiang Kun Peng Zhenyang Qin Yang Feng Bowen Huang Ping Ouyang Xiaoli Huang Defang Chen Weimin Lai Yi Geng |
| author_sort | Ziqi Tian |
| collection | DOAJ |
| description | <i>Vibrio mimicus</i> is a serious pathogen in aquatic animals, resulting in significant economic losses. The cAMP receptor protein (CRP) often acts as a central regulator in highly pathogenic pathogens. <i>V. mimicus</i> SCCF01 is a highly pathogenic strain isolated from yellow catfish; the <i>crp</i> gene deletion strain (Δ<i>crp</i>) was constructed by natural transformation to determine whether this deletion affects the virulence phenotypes. Their potential molecular connections were revealed by qRT-PCR analysis. Our results showed that the absence of the <i>crp</i> gene resulted in bacterial and colony morphological changes alongside decreases in bacterial growth, hemolytic activity, biofilm formation, enzymatic activity, motility, and cell adhesion. A cell cytotoxicity assay and animal experiments confirmed that <i>crp</i> contributes to <i>V. mimicus</i> pathogenicity, as the LD<sub>50</sub> of the Δ<i>crp</i> strain was 73.1-fold lower compared to the WT strain. Moreover, qRT-PCR analysis revealed the inhibition of type II secretion system genes, flagellum genes, adhesion genes, and metalloproteinase genes in the deletion strain. This resulted in the virulence phenotype differences described above. Together, these data demonstrate that the <i>crp</i> gene plays a core regulatory role in <i>V. mimicus</i> virulence and pathogenicity. |
| first_indexed | 2024-03-08T04:01:48Z |
| format | Article |
| id | doaj.art-8c6e14afd51a4c30a22756e6bcbd274a |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-08T04:01:48Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-8c6e14afd51a4c30a22756e6bcbd274a2024-02-09T15:06:40ZengMDPI AGAnimals2076-26152024-01-0114343710.3390/ani14030437The cAMP Receptor Protein (CRP) of <i>Vibrio mimicus</i> Regulates Its Bacterial Growth, Type II Secretion System, Flagellum Formation, Adhesion Genes, and VirulenceZiqi Tian0Fei Xiang1Kun Peng2Zhenyang Qin3Yang Feng4Bowen Huang5Ping Ouyang6Xiaoli Huang7Defang Chen8Weimin Lai9Yi Geng10College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaDepartment of Aquaculture, Sichuan Agricultural University, Chengdu 611130, ChinaDepartment of Aquaculture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China<i>Vibrio mimicus</i> is a serious pathogen in aquatic animals, resulting in significant economic losses. The cAMP receptor protein (CRP) often acts as a central regulator in highly pathogenic pathogens. <i>V. mimicus</i> SCCF01 is a highly pathogenic strain isolated from yellow catfish; the <i>crp</i> gene deletion strain (Δ<i>crp</i>) was constructed by natural transformation to determine whether this deletion affects the virulence phenotypes. Their potential molecular connections were revealed by qRT-PCR analysis. Our results showed that the absence of the <i>crp</i> gene resulted in bacterial and colony morphological changes alongside decreases in bacterial growth, hemolytic activity, biofilm formation, enzymatic activity, motility, and cell adhesion. A cell cytotoxicity assay and animal experiments confirmed that <i>crp</i> contributes to <i>V. mimicus</i> pathogenicity, as the LD<sub>50</sub> of the Δ<i>crp</i> strain was 73.1-fold lower compared to the WT strain. Moreover, qRT-PCR analysis revealed the inhibition of type II secretion system genes, flagellum genes, adhesion genes, and metalloproteinase genes in the deletion strain. This resulted in the virulence phenotype differences described above. Together, these data demonstrate that the <i>crp</i> gene plays a core regulatory role in <i>V. mimicus</i> virulence and pathogenicity.https://www.mdpi.com/2076-2615/14/3/437<i>Vibrio mimicus</i>cAMP receptor proteinphenotypepathogenicitytranscriptional regulation |
| spellingShingle | Ziqi Tian Fei Xiang Kun Peng Zhenyang Qin Yang Feng Bowen Huang Ping Ouyang Xiaoli Huang Defang Chen Weimin Lai Yi Geng The cAMP Receptor Protein (CRP) of <i>Vibrio mimicus</i> Regulates Its Bacterial Growth, Type II Secretion System, Flagellum Formation, Adhesion Genes, and Virulence Animals <i>Vibrio mimicus</i> cAMP receptor protein phenotype pathogenicity transcriptional regulation |
| title | The cAMP Receptor Protein (CRP) of <i>Vibrio mimicus</i> Regulates Its Bacterial Growth, Type II Secretion System, Flagellum Formation, Adhesion Genes, and Virulence |
| title_full | The cAMP Receptor Protein (CRP) of <i>Vibrio mimicus</i> Regulates Its Bacterial Growth, Type II Secretion System, Flagellum Formation, Adhesion Genes, and Virulence |
| title_fullStr | The cAMP Receptor Protein (CRP) of <i>Vibrio mimicus</i> Regulates Its Bacterial Growth, Type II Secretion System, Flagellum Formation, Adhesion Genes, and Virulence |
| title_full_unstemmed | The cAMP Receptor Protein (CRP) of <i>Vibrio mimicus</i> Regulates Its Bacterial Growth, Type II Secretion System, Flagellum Formation, Adhesion Genes, and Virulence |
| title_short | The cAMP Receptor Protein (CRP) of <i>Vibrio mimicus</i> Regulates Its Bacterial Growth, Type II Secretion System, Flagellum Formation, Adhesion Genes, and Virulence |
| title_sort | camp receptor protein crp of i vibrio mimicus i regulates its bacterial growth type ii secretion system flagellum formation adhesion genes and virulence |
| topic | <i>Vibrio mimicus</i> cAMP receptor protein phenotype pathogenicity transcriptional regulation |
| url | https://www.mdpi.com/2076-2615/14/3/437 |
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