Effects of Lake Sediment on Soil Properties, Crop Growth, and the phoD-Harboring Microbial Community
Removal of lake sediment has been shown to be an effective method for lake restoration. High phosphorus (P) content makes it possible for lake sediment to provide fertility for agricultural production. However, little research has focused on the responses of the soil-phosphorus-related microbial com...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Agriculture |
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| Online Access: | https://www.mdpi.com/2077-0472/12/12/2065 |
| _version_ | 1797461910045589504 |
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| author | Feng Zhong Naling Bai Xiangqian Chu Yu He Hanlin Zhang Haibo Li |
| author_facet | Feng Zhong Naling Bai Xiangqian Chu Yu He Hanlin Zhang Haibo Li |
| author_sort | Feng Zhong |
| collection | DOAJ |
| description | Removal of lake sediment has been shown to be an effective method for lake restoration. High phosphorus (P) content makes it possible for lake sediment to provide fertility for agricultural production. However, little research has focused on the responses of the soil-phosphorus-related microbial community to the sediment-derived fertilizer enriched in phosphorus content. The phoD-harboring gene, important to the global phosphorus cycle, encodes alkaline phosphatase hydrolyzing organic P in soil. Accordingly, a plot experiment was performed to compare the effects of four different fertilization treatments—no-fertilizer control (CK), 50% chemical fertilization with compressed sediment (CS), 50% chemical fertilization with original lake sediment (S), and conventional chemical fertilization treatment (CT)—on the phoD gene community using QPCR and high-throughput sequencing analysis. Relationships among soil physicochemical properties, phoD-harboring microbial community abundance and composition were also evaluated. Results showed that compared to CT, CS significantly increased soil organic matter (SOM) content by 20.29%, and S enhanced the humus content by 20.75% (<i>p</i> < 0.05). There was no significant influence on phoD gene microbial community richness (Chao1 and Sobs indexes) and diversity (Shannon index) between all treatments. The CS treatment significantly altered the phoD community structure and enhanced the Chinese cabbage yield by 40.19% (<i>p</i> < 0.05). Pearson analysis showed that phoD gene abundance (copy number) had significant and negative relationships with SOM, total nitrogen (TN), total phosphorus (TP), available nitrogen (AN), available phosphorus (AP), and the Chao1 index. Redundancy analysis showed that shifts in the phoD community structure were related to soil physicochemical properties (SOM, TN, TP, AN, AP, and humus) rather than soil pH. In conclusion, the compressed sediment can be used in farmland since it optimizes the phoD-harboring microbial community abundance, composition, and structure, and thus significantly increases the Chinese cabbage yield. |
| first_indexed | 2024-03-09T17:27:05Z |
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| id | doaj.art-8c71adfe52f5491f8b642f094f94aabd |
| institution | Directory Open Access Journal |
| issn | 2077-0472 |
| language | English |
| last_indexed | 2024-03-09T17:27:05Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agriculture |
| spelling | doaj.art-8c71adfe52f5491f8b642f094f94aabd2023-11-24T12:40:45ZengMDPI AGAgriculture2077-04722022-12-011212206510.3390/agriculture12122065Effects of Lake Sediment on Soil Properties, Crop Growth, and the phoD-Harboring Microbial CommunityFeng Zhong0Naling Bai1Xiangqian Chu2Yu He3Hanlin Zhang4Haibo Li5Faculty of Resources and Environmental Science, Hubei University, Wuhan 430062, ChinaInstitute of Eco-Environment and Plant Protection, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaCollege of Life Sciences, Shanghai Normal University, Shanghai 200234, ChinaInstitute of Eco-Environment and Plant Protection, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaInstitute of Eco-Environment and Plant Protection, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaFaculty of Resources and Environmental Science, Hubei University, Wuhan 430062, ChinaRemoval of lake sediment has been shown to be an effective method for lake restoration. High phosphorus (P) content makes it possible for lake sediment to provide fertility for agricultural production. However, little research has focused on the responses of the soil-phosphorus-related microbial community to the sediment-derived fertilizer enriched in phosphorus content. The phoD-harboring gene, important to the global phosphorus cycle, encodes alkaline phosphatase hydrolyzing organic P in soil. Accordingly, a plot experiment was performed to compare the effects of four different fertilization treatments—no-fertilizer control (CK), 50% chemical fertilization with compressed sediment (CS), 50% chemical fertilization with original lake sediment (S), and conventional chemical fertilization treatment (CT)—on the phoD gene community using QPCR and high-throughput sequencing analysis. Relationships among soil physicochemical properties, phoD-harboring microbial community abundance and composition were also evaluated. Results showed that compared to CT, CS significantly increased soil organic matter (SOM) content by 20.29%, and S enhanced the humus content by 20.75% (<i>p</i> < 0.05). There was no significant influence on phoD gene microbial community richness (Chao1 and Sobs indexes) and diversity (Shannon index) between all treatments. The CS treatment significantly altered the phoD community structure and enhanced the Chinese cabbage yield by 40.19% (<i>p</i> < 0.05). Pearson analysis showed that phoD gene abundance (copy number) had significant and negative relationships with SOM, total nitrogen (TN), total phosphorus (TP), available nitrogen (AN), available phosphorus (AP), and the Chao1 index. Redundancy analysis showed that shifts in the phoD community structure were related to soil physicochemical properties (SOM, TN, TP, AN, AP, and humus) rather than soil pH. In conclusion, the compressed sediment can be used in farmland since it optimizes the phoD-harboring microbial community abundance, composition, and structure, and thus significantly increases the Chinese cabbage yield.https://www.mdpi.com/2077-0472/12/12/2065lake sedimentphosphorusChinese cabbagephoD genehigh-throughput sequencing |
| spellingShingle | Feng Zhong Naling Bai Xiangqian Chu Yu He Hanlin Zhang Haibo Li Effects of Lake Sediment on Soil Properties, Crop Growth, and the phoD-Harboring Microbial Community Agriculture lake sediment phosphorus Chinese cabbage phoD gene high-throughput sequencing |
| title | Effects of Lake Sediment on Soil Properties, Crop Growth, and the phoD-Harboring Microbial Community |
| title_full | Effects of Lake Sediment on Soil Properties, Crop Growth, and the phoD-Harboring Microbial Community |
| title_fullStr | Effects of Lake Sediment on Soil Properties, Crop Growth, and the phoD-Harboring Microbial Community |
| title_full_unstemmed | Effects of Lake Sediment on Soil Properties, Crop Growth, and the phoD-Harboring Microbial Community |
| title_short | Effects of Lake Sediment on Soil Properties, Crop Growth, and the phoD-Harboring Microbial Community |
| title_sort | effects of lake sediment on soil properties crop growth and the phod harboring microbial community |
| topic | lake sediment phosphorus Chinese cabbage phoD gene high-throughput sequencing |
| url | https://www.mdpi.com/2077-0472/12/12/2065 |
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