Sequential Hydrothermal HCl Pretreatment and Enzymatic Hydrolysis of <i>Saccharina japonica</i> Biomass
This study investigated the production of fermentable sugars from carbohydrate-rich macroalgae <i>Saccharina japonica</i> using sequential hydrolysis (hydrothermal acid pretreatment and enzymatic hydrolysis) to determine the maximum reducing sugar yield (RS<inline-formula><math...
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MDPI AG
2021-12-01
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| Online Access: | https://www.mdpi.com/1996-1073/14/23/8053 |
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| author | Eun-Young Park Jung-Kyu Park |
| author_facet | Eun-Young Park Jung-Kyu Park |
| author_sort | Eun-Young Park |
| collection | DOAJ |
| description | This study investigated the production of fermentable sugars from carbohydrate-rich macroalgae <i>Saccharina japonica</i> using sequential hydrolysis (hydrothermal acid pretreatment and enzymatic hydrolysis) to determine the maximum reducing sugar yield (RS<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><msub><mrow></mrow><mi>y</mi></msub></semantics></math></inline-formula>). The sequential hydrolysis was predicted by three independent variables (temperature, time, and HCl concentration) using response surface methodology (RSM). Enzymatic hydrolysis (8.17% v/w<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><msub><mrow></mrow><mrow><mi>b</mi><mi>i</mi><mi>o</mi><mi>m</mi><mi>a</mi><mi>s</mi><mi>s</mi></mrow></msub></semantics></math></inline-formula> Celluclast<sup>®</sup> 1.5 L, 26.4 h, 42.6 °C) was performed after hydrothermal acid pretreatment under predicted conditions (143.6 °C, 22 min, and 0.108 N HCl concentration). Using this experimental procedure, the yields of hydrothermal acid pretreatment, enzymatic hydrolysis, and sequential hydrolysis were 115.6 ± 0.4 mg/g, 117.7 ± 0.3 mg/g, and 183.5 ± 0.6 mg/g, respectively. Our results suggested that sequential hydrolysis of hydrothermal acid pretreatment and enzymatic hydrolysis was more efficient than their single treatment. |
| first_indexed | 2024-03-10T04:54:26Z |
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| institution | Directory Open Access Journal |
| issn | 1996-1073 |
| language | English |
| last_indexed | 2024-03-10T04:54:26Z |
| publishDate | 2021-12-01 |
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| series | Energies |
| spelling | doaj.art-8c71b9b7a7a2476f9d286c8527b4a02f2023-11-23T02:21:56ZengMDPI AGEnergies1996-10732021-12-011423805310.3390/en14238053Sequential Hydrothermal HCl Pretreatment and Enzymatic Hydrolysis of <i>Saccharina japonica</i> BiomassEun-Young Park0Jung-Kyu Park1Rinascita College of Liberal Arts and Sciences, Shinhan University, Uijeongbu-si 11644, KoreaDepartment of Computer Software Engineering, Changshin University, Changwon-si 51352, KoreaThis study investigated the production of fermentable sugars from carbohydrate-rich macroalgae <i>Saccharina japonica</i> using sequential hydrolysis (hydrothermal acid pretreatment and enzymatic hydrolysis) to determine the maximum reducing sugar yield (RS<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><msub><mrow></mrow><mi>y</mi></msub></semantics></math></inline-formula>). The sequential hydrolysis was predicted by three independent variables (temperature, time, and HCl concentration) using response surface methodology (RSM). Enzymatic hydrolysis (8.17% v/w<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><msub><mrow></mrow><mrow><mi>b</mi><mi>i</mi><mi>o</mi><mi>m</mi><mi>a</mi><mi>s</mi><mi>s</mi></mrow></msub></semantics></math></inline-formula> Celluclast<sup>®</sup> 1.5 L, 26.4 h, 42.6 °C) was performed after hydrothermal acid pretreatment under predicted conditions (143.6 °C, 22 min, and 0.108 N HCl concentration). Using this experimental procedure, the yields of hydrothermal acid pretreatment, enzymatic hydrolysis, and sequential hydrolysis were 115.6 ± 0.4 mg/g, 117.7 ± 0.3 mg/g, and 183.5 ± 0.6 mg/g, respectively. Our results suggested that sequential hydrolysis of hydrothermal acid pretreatment and enzymatic hydrolysis was more efficient than their single treatment.https://www.mdpi.com/1996-1073/14/23/8053sequential hydrolysishydrothermal acid pretreatmentenzymatic hydrolysisreducing sugar yieldbiomass |
| spellingShingle | Eun-Young Park Jung-Kyu Park Sequential Hydrothermal HCl Pretreatment and Enzymatic Hydrolysis of <i>Saccharina japonica</i> Biomass Energies sequential hydrolysis hydrothermal acid pretreatment enzymatic hydrolysis reducing sugar yield biomass |
| title | Sequential Hydrothermal HCl Pretreatment and Enzymatic Hydrolysis of <i>Saccharina japonica</i> Biomass |
| title_full | Sequential Hydrothermal HCl Pretreatment and Enzymatic Hydrolysis of <i>Saccharina japonica</i> Biomass |
| title_fullStr | Sequential Hydrothermal HCl Pretreatment and Enzymatic Hydrolysis of <i>Saccharina japonica</i> Biomass |
| title_full_unstemmed | Sequential Hydrothermal HCl Pretreatment and Enzymatic Hydrolysis of <i>Saccharina japonica</i> Biomass |
| title_short | Sequential Hydrothermal HCl Pretreatment and Enzymatic Hydrolysis of <i>Saccharina japonica</i> Biomass |
| title_sort | sequential hydrothermal hcl pretreatment and enzymatic hydrolysis of i saccharina japonica i biomass |
| topic | sequential hydrolysis hydrothermal acid pretreatment enzymatic hydrolysis reducing sugar yield biomass |
| url | https://www.mdpi.com/1996-1073/14/23/8053 |
| work_keys_str_mv | AT eunyoungpark sequentialhydrothermalhclpretreatmentandenzymatichydrolysisofisaccharinajaponicaibiomass AT jungkyupark sequentialhydrothermalhclpretreatmentandenzymatichydrolysisofisaccharinajaponicaibiomass |