Comparative in vitro study of intracanal Enterococcus faecalis reduction using photosensitizers in aPDT
Aim: To compare Enterococcus faecalis reduction after antimicrobial photodynamic therapy (aPDT) used with methylene blue, toluidine blue, tannin, and curcumin as photosensitizers, an adjunct to endodontic chemomechanical preparation (CMP) in root canals of human teeth. Methods: A total of 120 single...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidade Estadual de Campinas
2021-08-01
|
| Series: | Brazilian Journal of Oral Sciences |
| Subjects: | |
| Online Access: | https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8664034 |
| _version_ | 1828933361556520960 |
|---|---|
| author | Maria Cleide Azevedo Braz André Hayato Saguchi Eduardo Akisue Adriana de Oliveira Lira Sidnea Aparecida Freitas Paiva Aldo Brugnera Junior Mary Caroline Skelton Macedo André Luiz da Costa Michelotto Ângela Toshie Araki |
| author_facet | Maria Cleide Azevedo Braz André Hayato Saguchi Eduardo Akisue Adriana de Oliveira Lira Sidnea Aparecida Freitas Paiva Aldo Brugnera Junior Mary Caroline Skelton Macedo André Luiz da Costa Michelotto Ângela Toshie Araki |
| author_sort | Maria Cleide Azevedo Braz |
| collection | DOAJ |
| description | Aim: To compare Enterococcus faecalis reduction after antimicrobial photodynamic therapy (aPDT) used with methylene blue, toluidine blue, tannin, and curcumin as photosensitizers, an adjunct to endodontic chemomechanical preparation (CMP) in root canals of human teeth. Methods: A total of 120 single-rooted teeth were divided into 6 groups (n = 20): G1- CMP and 2.5% sodium hypochlorite (NaOCl); G2- CMP and saline solution; G3- CMP, 2.5% NaOCl, and aPDT with 0.005% methylene blue; G4- CMP, 2.5% NaOCl, and aPDT with 0.005% toluidine blue; G5- CMP, 2.5% NaOCl, and aPDT with 0.005% tannin; and G6- CMP, 2.5% NaOCl, and aPDT with 0.005% curcumin. A portable semiconductor laser was used (660 nm, 100 mW, 1.8 J, 180s) in groups 1, 2, 3, 4, 5, and a blue LED light-curing (420-480 nm, 1200 mV/cm2 ) in G6. For all groups, a 5 min pre-irradiation time was applied. Samples were collected before (initial collection), immediately after (intermediate collection) and 7 days after CMP (final collection) for colony-forming unit (CFU) counting. The Kruskal-Wallis test and analysis of variance (ANOVA) were performed (p < 0.05; 95% confidence interval). Results: In between-group comparisons, there was no significant difference observed in the number of CFUs at the initial (p >< 0.001) and final collections (p >< 0.001) for G2 and G3. In within-group comparisons, the number of CFUs showed a decreasing trend in G4 (p = 0.007) and G5 (p = 0.001). Conclusion: Photosensitizers promoted E. faecalis reduction, with better results for tannin and curcumin. Alternative photosensitizers should be the focus of further studies.>< 0.05; 95% confidence interval). Results: In between-group comparisons, there was no significant difference observed in the number of CFUs at the initial (p < 0.001) and final collections (p >< 0.001) for G2 and G3. In within-group comparisons, the number of CFUs showed a decreasing trend in G4 (p = 0.007) and G5 (p = 0.001). Conclusion: Photosensitizers promoted E. faecalis reduction, with better results for tannin and curcumin. Alternative photosensitizers should be the focus of further studies.>< 0.001) and final collections (p < 0.001) for G2 and G3. In within-group comparisons, the number of CFUs showed a decreasing trend in G4 (p = 0.007) and G5 (p = 0.001). Conclusion: Photosensitizers promoted E. faecalis reduction, with better results for tannin and curcumin. Alternative photosensitizers should be the focus of further studies>< 0.001) for G2 and G3. In within-group comparisons, the number of CFUs showed a decreasing trend in G4 (p = 0.007) and G5 (p = 0.001). Conclusion: Photosensitizers promoted E. faecalis reduction, with better results for tannin and curcumin. Alternative photosensitizers should be the focus of further studies. |
| first_indexed | 2024-12-14T01:15:28Z |
| format | Article |
| id | doaj.art-8c7405f9f3ce4b519e21950c960a087c |
| institution | Directory Open Access Journal |
| issn | 1677-3225 |
| language | English |
| last_indexed | 2024-12-14T01:15:28Z |
| publishDate | 2021-08-01 |
| publisher | Universidade Estadual de Campinas |
| record_format | Article |
| series | Brazilian Journal of Oral Sciences |
| spelling | doaj.art-8c7405f9f3ce4b519e21950c960a087c2022-12-21T23:22:36ZengUniversidade Estadual de CampinasBrazilian Journal of Oral Sciences1677-32252021-08-01200010.20396/bjos.v20i00.8664034Comparative in vitro study of intracanal Enterococcus faecalis reduction using photosensitizers in aPDTMaria Cleide Azevedo Braz0André Hayato Saguchi1Eduardo Akisue2Adriana de Oliveira Lira3Sidnea Aparecida Freitas Paiva4Aldo Brugnera Junior5Mary Caroline Skelton Macedo6André Luiz da Costa Michelotto7Ângela Toshie Araki8Federal University of PatosCruzeiro do Sul UniversitySanta Cecilia UniversityCruzeiro do Sul UniversityCruzeiro do Sul UniversityCamilo Castelo Branco UniversityUniversity of São PauloFederal University of ParanáCruzeiro do Sul UniversityAim: To compare Enterococcus faecalis reduction after antimicrobial photodynamic therapy (aPDT) used with methylene blue, toluidine blue, tannin, and curcumin as photosensitizers, an adjunct to endodontic chemomechanical preparation (CMP) in root canals of human teeth. Methods: A total of 120 single-rooted teeth were divided into 6 groups (n = 20): G1- CMP and 2.5% sodium hypochlorite (NaOCl); G2- CMP and saline solution; G3- CMP, 2.5% NaOCl, and aPDT with 0.005% methylene blue; G4- CMP, 2.5% NaOCl, and aPDT with 0.005% toluidine blue; G5- CMP, 2.5% NaOCl, and aPDT with 0.005% tannin; and G6- CMP, 2.5% NaOCl, and aPDT with 0.005% curcumin. A portable semiconductor laser was used (660 nm, 100 mW, 1.8 J, 180s) in groups 1, 2, 3, 4, 5, and a blue LED light-curing (420-480 nm, 1200 mV/cm2 ) in G6. For all groups, a 5 min pre-irradiation time was applied. Samples were collected before (initial collection), immediately after (intermediate collection) and 7 days after CMP (final collection) for colony-forming unit (CFU) counting. The Kruskal-Wallis test and analysis of variance (ANOVA) were performed (p < 0.05; 95% confidence interval). Results: In between-group comparisons, there was no significant difference observed in the number of CFUs at the initial (p >< 0.001) and final collections (p >< 0.001) for G2 and G3. In within-group comparisons, the number of CFUs showed a decreasing trend in G4 (p = 0.007) and G5 (p = 0.001). Conclusion: Photosensitizers promoted E. faecalis reduction, with better results for tannin and curcumin. Alternative photosensitizers should be the focus of further studies.>< 0.05; 95% confidence interval). Results: In between-group comparisons, there was no significant difference observed in the number of CFUs at the initial (p < 0.001) and final collections (p >< 0.001) for G2 and G3. In within-group comparisons, the number of CFUs showed a decreasing trend in G4 (p = 0.007) and G5 (p = 0.001). Conclusion: Photosensitizers promoted E. faecalis reduction, with better results for tannin and curcumin. Alternative photosensitizers should be the focus of further studies.>< 0.001) and final collections (p < 0.001) for G2 and G3. In within-group comparisons, the number of CFUs showed a decreasing trend in G4 (p = 0.007) and G5 (p = 0.001). Conclusion: Photosensitizers promoted E. faecalis reduction, with better results for tannin and curcumin. Alternative photosensitizers should be the focus of further studies>< 0.001) for G2 and G3. In within-group comparisons, the number of CFUs showed a decreasing trend in G4 (p = 0.007) and G5 (p = 0.001). Conclusion: Photosensitizers promoted E. faecalis reduction, with better results for tannin and curcumin. Alternative photosensitizers should be the focus of further studies.https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8664034PhotodynamicEndodonticsPhotosensitizing agentsCurcuminTannins |
| spellingShingle | Maria Cleide Azevedo Braz André Hayato Saguchi Eduardo Akisue Adriana de Oliveira Lira Sidnea Aparecida Freitas Paiva Aldo Brugnera Junior Mary Caroline Skelton Macedo André Luiz da Costa Michelotto Ângela Toshie Araki Comparative in vitro study of intracanal Enterococcus faecalis reduction using photosensitizers in aPDT Brazilian Journal of Oral Sciences Photodynamic Endodontics Photosensitizing agents Curcumin Tannins |
| title | Comparative in vitro study of intracanal Enterococcus faecalis reduction using photosensitizers in aPDT |
| title_full | Comparative in vitro study of intracanal Enterococcus faecalis reduction using photosensitizers in aPDT |
| title_fullStr | Comparative in vitro study of intracanal Enterococcus faecalis reduction using photosensitizers in aPDT |
| title_full_unstemmed | Comparative in vitro study of intracanal Enterococcus faecalis reduction using photosensitizers in aPDT |
| title_short | Comparative in vitro study of intracanal Enterococcus faecalis reduction using photosensitizers in aPDT |
| title_sort | comparative in vitro study of intracanal enterococcus faecalis reduction using photosensitizers in apdt |
| topic | Photodynamic Endodontics Photosensitizing agents Curcumin Tannins |
| url | https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8664034 |
| work_keys_str_mv | AT mariacleideazevedobraz comparativeinvitrostudyofintracanalenterococcusfaecalisreductionusingphotosensitizersinapdt AT andrehayatosaguchi comparativeinvitrostudyofintracanalenterococcusfaecalisreductionusingphotosensitizersinapdt AT eduardoakisue comparativeinvitrostudyofintracanalenterococcusfaecalisreductionusingphotosensitizersinapdt AT adrianadeoliveiralira comparativeinvitrostudyofintracanalenterococcusfaecalisreductionusingphotosensitizersinapdt AT sidneaaparecidafreitaspaiva comparativeinvitrostudyofintracanalenterococcusfaecalisreductionusingphotosensitizersinapdt AT aldobrugnerajunior comparativeinvitrostudyofintracanalenterococcusfaecalisreductionusingphotosensitizersinapdt AT marycarolineskeltonmacedo comparativeinvitrostudyofintracanalenterococcusfaecalisreductionusingphotosensitizersinapdt AT andreluizdacostamichelotto comparativeinvitrostudyofintracanalenterococcusfaecalisreductionusingphotosensitizersinapdt AT angelatoshiearaki comparativeinvitrostudyofintracanalenterococcusfaecalisreductionusingphotosensitizersinapdt |