Evidence for rate‐dependent filtering of global extrinsic noise by biochemical reactions in mammalian cells
Abstract Recent studies have revealed that global extrinsic noise arising from stochasticity in the intracellular biochemical environment plays a critical role in heterogeneous cell physiologies. However, it remains largely unclear how such extrinsic noise dynamically influences downstream reactions...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Springer Nature
2020-05-01
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| Series: | Molecular Systems Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.15252/msb.20199335 |
| _version_ | 1797282503005831168 |
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| author | Jiegen Wu Xu Han Haotian Zhai Tingyu Yang Yihan Lin |
| author_facet | Jiegen Wu Xu Han Haotian Zhai Tingyu Yang Yihan Lin |
| author_sort | Jiegen Wu |
| collection | DOAJ |
| description | Abstract Recent studies have revealed that global extrinsic noise arising from stochasticity in the intracellular biochemical environment plays a critical role in heterogeneous cell physiologies. However, it remains largely unclear how such extrinsic noise dynamically influences downstream reactions and whether it could be neutralized by cellular reactions. Here, using fluorescent protein (FP) maturation as a model biochemical reaction, we explored how cellular reactions might combat global extrinsic noise in mammalian cells. We developed a novel single‐cell assay to systematically quantify the maturation rate and the associated noise for over a dozen FPs. By exploiting the variation in the maturation rate for different FPs, we inferred that global extrinsic noise could be temporally filtered by maturation reactions, and as a result, the noise levels for slow‐maturing FPs are lower compared to fast‐maturing FPs. This mechanism is validated by directly perturbing the maturation rates of specific FPs and measuring the resulting noise levels. Together, our results revealed a potentially general principle governing extrinsic noise propagation, where timescale separation allows cellular reactions to cope with dynamic global extrinsic noise. |
| first_indexed | 2024-03-07T17:13:59Z |
| format | Article |
| id | doaj.art-8c75f3c3af844a4c87fa587e580dba83 |
| institution | Directory Open Access Journal |
| issn | 1744-4292 |
| language | English |
| last_indexed | 2024-03-07T17:13:59Z |
| publishDate | 2020-05-01 |
| publisher | Springer Nature |
| record_format | Article |
| series | Molecular Systems Biology |
| spelling | doaj.art-8c75f3c3af844a4c87fa587e580dba832024-03-02T23:49:15ZengSpringer NatureMolecular Systems Biology1744-42922020-05-01165n/an/a10.15252/msb.20199335Evidence for rate‐dependent filtering of global extrinsic noise by biochemical reactions in mammalian cellsJiegen Wu0Xu Han1Haotian Zhai2Tingyu Yang3Yihan Lin4Center for Quantitative Biology and Peking‐Tsinghua Joint Center for Life Sciences Academy for Advanced Interdisciplinary Studies Peking University Beijing ChinaCenter for Quantitative Biology and Peking‐Tsinghua Joint Center for Life Sciences Academy for Advanced Interdisciplinary Studies Peking University Beijing ChinaCenter for Quantitative Biology and Peking‐Tsinghua Joint Center for Life Sciences Academy for Advanced Interdisciplinary Studies Peking University Beijing ChinaCenter for Quantitative Biology and Peking‐Tsinghua Joint Center for Life Sciences Academy for Advanced Interdisciplinary Studies Peking University Beijing ChinaCenter for Quantitative Biology and Peking‐Tsinghua Joint Center for Life Sciences Academy for Advanced Interdisciplinary Studies Peking University Beijing ChinaAbstract Recent studies have revealed that global extrinsic noise arising from stochasticity in the intracellular biochemical environment plays a critical role in heterogeneous cell physiologies. However, it remains largely unclear how such extrinsic noise dynamically influences downstream reactions and whether it could be neutralized by cellular reactions. Here, using fluorescent protein (FP) maturation as a model biochemical reaction, we explored how cellular reactions might combat global extrinsic noise in mammalian cells. We developed a novel single‐cell assay to systematically quantify the maturation rate and the associated noise for over a dozen FPs. By exploiting the variation in the maturation rate for different FPs, we inferred that global extrinsic noise could be temporally filtered by maturation reactions, and as a result, the noise levels for slow‐maturing FPs are lower compared to fast‐maturing FPs. This mechanism is validated by directly perturbing the maturation rates of specific FPs and measuring the resulting noise levels. Together, our results revealed a potentially general principle governing extrinsic noise propagation, where timescale separation allows cellular reactions to cope with dynamic global extrinsic noise.https://doi.org/10.15252/msb.20199335biological noisechromophore maturationfluorescent proteinglobal extrinsic noise |
| spellingShingle | Jiegen Wu Xu Han Haotian Zhai Tingyu Yang Yihan Lin Evidence for rate‐dependent filtering of global extrinsic noise by biochemical reactions in mammalian cells Molecular Systems Biology biological noise chromophore maturation fluorescent protein global extrinsic noise |
| title | Evidence for rate‐dependent filtering of global extrinsic noise by biochemical reactions in mammalian cells |
| title_full | Evidence for rate‐dependent filtering of global extrinsic noise by biochemical reactions in mammalian cells |
| title_fullStr | Evidence for rate‐dependent filtering of global extrinsic noise by biochemical reactions in mammalian cells |
| title_full_unstemmed | Evidence for rate‐dependent filtering of global extrinsic noise by biochemical reactions in mammalian cells |
| title_short | Evidence for rate‐dependent filtering of global extrinsic noise by biochemical reactions in mammalian cells |
| title_sort | evidence for rate dependent filtering of global extrinsic noise by biochemical reactions in mammalian cells |
| topic | biological noise chromophore maturation fluorescent protein global extrinsic noise |
| url | https://doi.org/10.15252/msb.20199335 |
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