The Effects of SRT1720 Treatment on Endothelial Cells Derived from the Lung and Bone Marrow of Young and Aged, Male and Female Mice
Angiogenesis is critical for successful fracture healing. Age-related alterations in endothelial cells (ECs) may cause impaired bone healing. Therefore, examining therapeutic treatments to improve angiogenesis in aging may enhance bone healing. Sirtuin 1 (SIRT1) is highly expressed in ECs and its ac...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/20/11097 |
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| author | Ushashi Chand Dadwal Fazal Ur Rehman Bhatti Olatundun Dupe Awosanya Caio de Andrade Staut Rohit U. Nagaraj Anthony Joseph Perugini Nikhil Prasad Tewari Conner Riley Valuch Seungyup Sun Stephen Kyle Mendenhall Donghui Zhou Sarah Lyn Mostardo Rachel Jean Blosser Jiliang Li Melissa Ann Kacena |
| author_facet | Ushashi Chand Dadwal Fazal Ur Rehman Bhatti Olatundun Dupe Awosanya Caio de Andrade Staut Rohit U. Nagaraj Anthony Joseph Perugini Nikhil Prasad Tewari Conner Riley Valuch Seungyup Sun Stephen Kyle Mendenhall Donghui Zhou Sarah Lyn Mostardo Rachel Jean Blosser Jiliang Li Melissa Ann Kacena |
| author_sort | Ushashi Chand Dadwal |
| collection | DOAJ |
| description | Angiogenesis is critical for successful fracture healing. Age-related alterations in endothelial cells (ECs) may cause impaired bone healing. Therefore, examining therapeutic treatments to improve angiogenesis in aging may enhance bone healing. Sirtuin 1 (SIRT1) is highly expressed in ECs and its activation is known to counteract aging. Here, we examined the effects of SRT1720 treatment (SIRT1 activator) on the growth and function of bone marrow and lung ECs (BMECs and LECs, respectively), derived from young (3–4 month) and old (20–24 month) mice. While aging did not alter EC proliferation, treatment with SRT1720 significantly increased proliferation of all LECs. However, SRT1720 only increased proliferation of old female BMECs. Vessel-like tube assays showed similar vessel-like structures between young and old LECs and BMECs from both male and female mice. SRT1720 significantly improved vessel-like structures in all LECs. No age, sex, or treatment differences were found in migration related parameters of LECs. In males, old BMECs had greater migration rates than young BMECs, whereas in females, old BMECs had lower migration rates than young BMECs. Collectively, our data suggest that treatment with SRT1720 appears to enhance the angiogenic potential of LECs irrespective of age or sex. However, its role in BMECs is sex- and age-dependent. |
| first_indexed | 2024-03-10T06:30:54Z |
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| id | doaj.art-8c7724f8e35e4fa8bf3fff276300a6b2 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T06:30:54Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-8c7724f8e35e4fa8bf3fff276300a6b22023-11-22T18:33:49ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-10-0122201109710.3390/ijms222011097The Effects of SRT1720 Treatment on Endothelial Cells Derived from the Lung and Bone Marrow of Young and Aged, Male and Female MiceUshashi Chand Dadwal0Fazal Ur Rehman Bhatti1Olatundun Dupe Awosanya2Caio de Andrade Staut3Rohit U. Nagaraj4Anthony Joseph Perugini5Nikhil Prasad Tewari6Conner Riley Valuch7Seungyup Sun8Stephen Kyle Mendenhall9Donghui Zhou10Sarah Lyn Mostardo11Rachel Jean Blosser12Jiliang Li13Melissa Ann Kacena14Department of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN 46202, USADepartment of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN 46202, USADepartment of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN 46202, USADepartment of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN 46202, USADepartment of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN 46202, USADepartment of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN 46202, USADepartment of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN 46202, USADepartment of Biology, Indiana University Purdue University Indianapolis, Indianapolis, IN 46202, USADepartment of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN 46202, USADepartment of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN 46202, USADepartment of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN 46202, USADepartment of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN 46202, USADepartment of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN 46202, USADepartment of Biology, Indiana University Purdue University Indianapolis, Indianapolis, IN 46202, USADepartment of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN 46202, USAAngiogenesis is critical for successful fracture healing. Age-related alterations in endothelial cells (ECs) may cause impaired bone healing. Therefore, examining therapeutic treatments to improve angiogenesis in aging may enhance bone healing. Sirtuin 1 (SIRT1) is highly expressed in ECs and its activation is known to counteract aging. Here, we examined the effects of SRT1720 treatment (SIRT1 activator) on the growth and function of bone marrow and lung ECs (BMECs and LECs, respectively), derived from young (3–4 month) and old (20–24 month) mice. While aging did not alter EC proliferation, treatment with SRT1720 significantly increased proliferation of all LECs. However, SRT1720 only increased proliferation of old female BMECs. Vessel-like tube assays showed similar vessel-like structures between young and old LECs and BMECs from both male and female mice. SRT1720 significantly improved vessel-like structures in all LECs. No age, sex, or treatment differences were found in migration related parameters of LECs. In males, old BMECs had greater migration rates than young BMECs, whereas in females, old BMECs had lower migration rates than young BMECs. Collectively, our data suggest that treatment with SRT1720 appears to enhance the angiogenic potential of LECs irrespective of age or sex. However, its role in BMECs is sex- and age-dependent.https://www.mdpi.com/1422-0067/22/20/11097angiogenesisendothelial cellsbonelungsagingSirtuin 1 |
| spellingShingle | Ushashi Chand Dadwal Fazal Ur Rehman Bhatti Olatundun Dupe Awosanya Caio de Andrade Staut Rohit U. Nagaraj Anthony Joseph Perugini Nikhil Prasad Tewari Conner Riley Valuch Seungyup Sun Stephen Kyle Mendenhall Donghui Zhou Sarah Lyn Mostardo Rachel Jean Blosser Jiliang Li Melissa Ann Kacena The Effects of SRT1720 Treatment on Endothelial Cells Derived from the Lung and Bone Marrow of Young and Aged, Male and Female Mice International Journal of Molecular Sciences angiogenesis endothelial cells bone lungs aging Sirtuin 1 |
| title | The Effects of SRT1720 Treatment on Endothelial Cells Derived from the Lung and Bone Marrow of Young and Aged, Male and Female Mice |
| title_full | The Effects of SRT1720 Treatment on Endothelial Cells Derived from the Lung and Bone Marrow of Young and Aged, Male and Female Mice |
| title_fullStr | The Effects of SRT1720 Treatment on Endothelial Cells Derived from the Lung and Bone Marrow of Young and Aged, Male and Female Mice |
| title_full_unstemmed | The Effects of SRT1720 Treatment on Endothelial Cells Derived from the Lung and Bone Marrow of Young and Aged, Male and Female Mice |
| title_short | The Effects of SRT1720 Treatment on Endothelial Cells Derived from the Lung and Bone Marrow of Young and Aged, Male and Female Mice |
| title_sort | effects of srt1720 treatment on endothelial cells derived from the lung and bone marrow of young and aged male and female mice |
| topic | angiogenesis endothelial cells bone lungs aging Sirtuin 1 |
| url | https://www.mdpi.com/1422-0067/22/20/11097 |
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