Novel suspension retroviral packaging cells generated by transposition using transposase encoding mRNA advance vector yields and enable production in bioreactors

Novel suspension retroviral packaging cells generated by transposition using transposase encoding mRNA advance vector yields and enable production in bioreactors

To date, the establishment of high-titer stable viral packaging cells (VPCs) at large scale for gene therapeutic applications is very time- and cost-intensive. Here we report the establishment of three human suspension 293-F-derived ecotropic MLV-based VPCs. The classic stable transfection of an EGF...

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Bibliographic Details
Main Authors: Yasemin van Heuvel, Stefanie Schatz, Marc Hein, Tanya Dogra, Daniel Kazenmaier, Natalie Tschorn, Yvonne Genzel, Jörn Stitz
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-04-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
sleeping beauty transposon
mRNA transfection
suspension cell
retroviral vector
murine leukemia virus (MLV)
stirred-tank bioreactor
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2023.1076524/full

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