Effects of dicopper oxide and copper sulfate on growth performance and gut microbiota in broilers
ABSTRACT: An experiment was conducted to determine the effects of two sources of copper (Cu) from copper sulfate (CuSO4) and dicopper oxide (Cu2O, CoRouge) at three levels of inclusion (15, 75, and 150 mg/kg) on growth performance and gut microbiota of broilers. A total of 840 one-d-old male chicken...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-08-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579121002583 |
| _version_ | 1818657993033515008 |
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| author | A. Forouzandeh L. Blavi N. Abdelli D. Melo-Duran A. Vidal M. Rodríguez A.N.T.R. Monteiro J.F. Pérez L. Darwich D. Solà-Oriol |
| author_facet | A. Forouzandeh L. Blavi N. Abdelli D. Melo-Duran A. Vidal M. Rodríguez A.N.T.R. Monteiro J.F. Pérez L. Darwich D. Solà-Oriol |
| author_sort | A. Forouzandeh |
| collection | DOAJ |
| description | ABSTRACT: An experiment was conducted to determine the effects of two sources of copper (Cu) from copper sulfate (CuSO4) and dicopper oxide (Cu2O, CoRouge) at three levels of inclusion (15, 75, and 150 mg/kg) on growth performance and gut microbiota of broilers. A total of 840 one-d-old male chickens (Ross 308) were weighed and randomly allocated to seven dietary treatments: negative control (NC, a basal diet without Cu addition), and the NC supplemented with 15, 75, or 150 mg Cu/kg from CuSO4 or Cu2O (12 replicate pens/treatment, 10 chicks per pen). Broilers were challenged by reusing an old litter with high concentrations in Clostridium perfringens to promote necrotic enteritis. Broiler performance was registered at d 21, 35, and 42. Excreta samples were collected at d 14, 28, and 42 for antimicrobial resistance (AMR) analyses. At d 43, one broiler per pen was euthanized to obtain ileal content for microbial characterization. Body weight d 35 and daily gain d 42 improved (P < 0.05) in Cu2O as Cu dose inclusion increased from 15 mg/kg to 150 mg/kg. Supplementation of 150 mg/kg of Cu from Cu2O decreased the abundance (P < 0.01) of some families such as Streptococcaceae and Corynebacteriaceae and increased the abundance (P < 0.05) of some commensal bacteria like Clostridiaceae and Peptostreptococcaceae. Phenotypic AMR was not different among treatments on d 14 and 28. Isolated Enterococcus spp. from broilers fed the NC diet on d 42 showed higher (P < 0.05) resistance to enrofloxacin, gentamicin, and chloramphenicol compared with Cu treatments. By contrast, the isolated Escherichia coli from broilers fed 150 mg/kg of Cu, either from CuSO4 or Cu2O, showed higher (P < 0.05) resistance to streptomycin and chloramphenicol compared to the NC. This study suggests that supplementing 150 mg/kg of Cu from Cu2O establishes changes in the gut microbiota by regulating the bacterial population in the ileum, which may explain the positive impact on broilers' growth performance. |
| first_indexed | 2024-12-17T03:50:18Z |
| format | Article |
| id | doaj.art-8c783f05289a4daabf651972a25c5428 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-12-17T03:50:18Z |
| publishDate | 2021-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-8c783f05289a4daabf651972a25c54282022-12-21T22:04:47ZengElsevierPoultry Science0032-57912021-08-011008101224Effects of dicopper oxide and copper sulfate on growth performance and gut microbiota in broilersA. Forouzandeh0L. Blavi1N. Abdelli2D. Melo-Duran3A. Vidal4M. Rodríguez5A.N.T.R. Monteiro6J.F. Pérez7L. Darwich8D. Solà-Oriol9Animal Nutrition and Welfare Service (SNiBA), Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainAnimal Nutrition and Welfare Service (SNiBA), Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain; Corresponding author:Animal Nutrition and Welfare Service (SNiBA), Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainAnimal Nutrition and Welfare Service (SNiBA), Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainDepartment of Animal Health and Anatomy, Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainPigCHAMP Pro Europa, Segovia, SpainAnimine, Annecy, FranceAnimal Nutrition and Welfare Service (SNiBA), Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainDepartment of Animal Health and Anatomy, Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainAnimal Nutrition and Welfare Service (SNiBA), Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainABSTRACT: An experiment was conducted to determine the effects of two sources of copper (Cu) from copper sulfate (CuSO4) and dicopper oxide (Cu2O, CoRouge) at three levels of inclusion (15, 75, and 150 mg/kg) on growth performance and gut microbiota of broilers. A total of 840 one-d-old male chickens (Ross 308) were weighed and randomly allocated to seven dietary treatments: negative control (NC, a basal diet without Cu addition), and the NC supplemented with 15, 75, or 150 mg Cu/kg from CuSO4 or Cu2O (12 replicate pens/treatment, 10 chicks per pen). Broilers were challenged by reusing an old litter with high concentrations in Clostridium perfringens to promote necrotic enteritis. Broiler performance was registered at d 21, 35, and 42. Excreta samples were collected at d 14, 28, and 42 for antimicrobial resistance (AMR) analyses. At d 43, one broiler per pen was euthanized to obtain ileal content for microbial characterization. Body weight d 35 and daily gain d 42 improved (P < 0.05) in Cu2O as Cu dose inclusion increased from 15 mg/kg to 150 mg/kg. Supplementation of 150 mg/kg of Cu from Cu2O decreased the abundance (P < 0.01) of some families such as Streptococcaceae and Corynebacteriaceae and increased the abundance (P < 0.05) of some commensal bacteria like Clostridiaceae and Peptostreptococcaceae. Phenotypic AMR was not different among treatments on d 14 and 28. Isolated Enterococcus spp. from broilers fed the NC diet on d 42 showed higher (P < 0.05) resistance to enrofloxacin, gentamicin, and chloramphenicol compared with Cu treatments. By contrast, the isolated Escherichia coli from broilers fed 150 mg/kg of Cu, either from CuSO4 or Cu2O, showed higher (P < 0.05) resistance to streptomycin and chloramphenicol compared to the NC. This study suggests that supplementing 150 mg/kg of Cu from Cu2O establishes changes in the gut microbiota by regulating the bacterial population in the ileum, which may explain the positive impact on broilers' growth performance.http://www.sciencedirect.com/science/article/pii/S0032579121002583copperbroilergrowth performanceileal microbiotaantimicrobial resistance |
| spellingShingle | A. Forouzandeh L. Blavi N. Abdelli D. Melo-Duran A. Vidal M. Rodríguez A.N.T.R. Monteiro J.F. Pérez L. Darwich D. Solà-Oriol Effects of dicopper oxide and copper sulfate on growth performance and gut microbiota in broilers Poultry Science copper broiler growth performance ileal microbiota antimicrobial resistance |
| title | Effects of dicopper oxide and copper sulfate on growth performance and gut microbiota in broilers |
| title_full | Effects of dicopper oxide and copper sulfate on growth performance and gut microbiota in broilers |
| title_fullStr | Effects of dicopper oxide and copper sulfate on growth performance and gut microbiota in broilers |
| title_full_unstemmed | Effects of dicopper oxide and copper sulfate on growth performance and gut microbiota in broilers |
| title_short | Effects of dicopper oxide and copper sulfate on growth performance and gut microbiota in broilers |
| title_sort | effects of dicopper oxide and copper sulfate on growth performance and gut microbiota in broilers |
| topic | copper broiler growth performance ileal microbiota antimicrobial resistance |
| url | http://www.sciencedirect.com/science/article/pii/S0032579121002583 |
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