Electrophysiological and behavioral characterization of bioactive compounds of the Thymus vulgaris, Cymbopogon winterianus, Cuminum cyminum and Cinnamomum zeylanicum essential oils against Anopheles gambiae and prospects for their use as bednet treatments
Abstract Background Laboratory and field studies showed that repellent, irritant and toxic actions of common public health insecticides reduce human-vector contact and thereby interrupt disease transmission. One of the more effective strategies to reduce disease risk involves the use of long-lasting...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2015-06-01
|
| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13071-015-0934-y |
| _version_ | 1797811827066798080 |
|---|---|
| author | Emilie Deletre Fabrice Chandre Livy Williams Claire Duménil Chantal Menut Thibaud Martin |
| author_facet | Emilie Deletre Fabrice Chandre Livy Williams Claire Duménil Chantal Menut Thibaud Martin |
| author_sort | Emilie Deletre |
| collection | DOAJ |
| description | Abstract Background Laboratory and field studies showed that repellent, irritant and toxic actions of common public health insecticides reduce human-vector contact and thereby interrupt disease transmission. One of the more effective strategies to reduce disease risk involves the use of long-lasting treated bednets. However, development of insecticide resistance in mosquito populations makes it imperative to find alternatives to these insecticides. Our previous study identified four essential oils as alternatives to pyrethroids: Thymus vulgaris, Cymbopogon winterianus, Cuminum cyminum, Cinnamomum zeylanicum. The objectives of this study were to identify active compounds of these essential oils, to characterize their biological activity, and to examine their potential as a treatment for bednets. Methods We evaluated the electrophysiological, behavioural (repellency, irritancy) and toxic effects of the major compounds of these oils against Anopheles gambiae strain ‘Kisumu’. Results Aldehydes elicited the strongest responses and monoterpenes the weakest responses in electroantennogram (EAG) trials. However, EAG responses did not correlate consistently with results of behavioral assays. In behavioral and toxicity studies, several of the single compounds did exhibit repellency, irritancy or toxicity in An. gambiae; however, the activity of essential oils did not always correlate with activity expected from the major components. On the contrary, the biological activity of essential oils appeared complex, suggesting interactions between individual compounds and the insect under study. Data also indicated that the three effects appeared independent, suggesting that repellency mechanism(s) may differ from mechanisms of irritancy and toxicity. Conclusions Based on the bioassays reported here, some of the compounds merit consideration as alternative bednet treatments. |
| first_indexed | 2024-03-13T07:29:34Z |
| format | Article |
| id | doaj.art-8c78735f277b4c42ba10f61b8a781a86 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-03-13T07:29:34Z |
| publishDate | 2015-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-8c78735f277b4c42ba10f61b8a781a862023-06-04T11:11:00ZengBMCParasites & Vectors1756-33052015-06-018111410.1186/s13071-015-0934-yElectrophysiological and behavioral characterization of bioactive compounds of the Thymus vulgaris, Cymbopogon winterianus, Cuminum cyminum and Cinnamomum zeylanicum essential oils against Anopheles gambiae and prospects for their use as bednet treatmentsEmilie Deletre0Fabrice Chandre1Livy Williams2Claire Duménil3Chantal Menut4Thibaud Martin5UR Hortsys, CiradUMR MiVEGEC, IRD-CNRS-UM1-UM2USDA-ARS, European Biological Control LaboratoryUR Hortsys, CiradInstitut des Biomolécules Max Mousseron, Faculté de PharmacieUR Hortsys, CiradAbstract Background Laboratory and field studies showed that repellent, irritant and toxic actions of common public health insecticides reduce human-vector contact and thereby interrupt disease transmission. One of the more effective strategies to reduce disease risk involves the use of long-lasting treated bednets. However, development of insecticide resistance in mosquito populations makes it imperative to find alternatives to these insecticides. Our previous study identified four essential oils as alternatives to pyrethroids: Thymus vulgaris, Cymbopogon winterianus, Cuminum cyminum, Cinnamomum zeylanicum. The objectives of this study were to identify active compounds of these essential oils, to characterize their biological activity, and to examine their potential as a treatment for bednets. Methods We evaluated the electrophysiological, behavioural (repellency, irritancy) and toxic effects of the major compounds of these oils against Anopheles gambiae strain ‘Kisumu’. Results Aldehydes elicited the strongest responses and monoterpenes the weakest responses in electroantennogram (EAG) trials. However, EAG responses did not correlate consistently with results of behavioral assays. In behavioral and toxicity studies, several of the single compounds did exhibit repellency, irritancy or toxicity in An. gambiae; however, the activity of essential oils did not always correlate with activity expected from the major components. On the contrary, the biological activity of essential oils appeared complex, suggesting interactions between individual compounds and the insect under study. Data also indicated that the three effects appeared independent, suggesting that repellency mechanism(s) may differ from mechanisms of irritancy and toxicity. Conclusions Based on the bioassays reported here, some of the compounds merit consideration as alternative bednet treatments.https://doi.org/10.1186/s13071-015-0934-yEssential oilRepellencyToxicityVector controlDEETPermethrin |
| spellingShingle | Emilie Deletre Fabrice Chandre Livy Williams Claire Duménil Chantal Menut Thibaud Martin Electrophysiological and behavioral characterization of bioactive compounds of the Thymus vulgaris, Cymbopogon winterianus, Cuminum cyminum and Cinnamomum zeylanicum essential oils against Anopheles gambiae and prospects for their use as bednet treatments Parasites & Vectors Essential oil Repellency Toxicity Vector control DEET Permethrin |
| title | Electrophysiological and behavioral characterization of bioactive compounds of the Thymus vulgaris, Cymbopogon winterianus, Cuminum cyminum and Cinnamomum zeylanicum essential oils against Anopheles gambiae and prospects for their use as bednet treatments |
| title_full | Electrophysiological and behavioral characterization of bioactive compounds of the Thymus vulgaris, Cymbopogon winterianus, Cuminum cyminum and Cinnamomum zeylanicum essential oils against Anopheles gambiae and prospects for their use as bednet treatments |
| title_fullStr | Electrophysiological and behavioral characterization of bioactive compounds of the Thymus vulgaris, Cymbopogon winterianus, Cuminum cyminum and Cinnamomum zeylanicum essential oils against Anopheles gambiae and prospects for their use as bednet treatments |
| title_full_unstemmed | Electrophysiological and behavioral characterization of bioactive compounds of the Thymus vulgaris, Cymbopogon winterianus, Cuminum cyminum and Cinnamomum zeylanicum essential oils against Anopheles gambiae and prospects for their use as bednet treatments |
| title_short | Electrophysiological and behavioral characterization of bioactive compounds of the Thymus vulgaris, Cymbopogon winterianus, Cuminum cyminum and Cinnamomum zeylanicum essential oils against Anopheles gambiae and prospects for their use as bednet treatments |
| title_sort | electrophysiological and behavioral characterization of bioactive compounds of the thymus vulgaris cymbopogon winterianus cuminum cyminum and cinnamomum zeylanicum essential oils against anopheles gambiae and prospects for their use as bednet treatments |
| topic | Essential oil Repellency Toxicity Vector control DEET Permethrin |
| url | https://doi.org/10.1186/s13071-015-0934-y |
| work_keys_str_mv | AT emiliedeletre electrophysiologicalandbehavioralcharacterizationofbioactivecompoundsofthethymusvulgariscymbopogonwinterianuscuminumcyminumandcinnamomumzeylanicumessentialoilsagainstanophelesgambiaeandprospectsfortheiruseasbednettreatments AT fabricechandre electrophysiologicalandbehavioralcharacterizationofbioactivecompoundsofthethymusvulgariscymbopogonwinterianuscuminumcyminumandcinnamomumzeylanicumessentialoilsagainstanophelesgambiaeandprospectsfortheiruseasbednettreatments AT livywilliams electrophysiologicalandbehavioralcharacterizationofbioactivecompoundsofthethymusvulgariscymbopogonwinterianuscuminumcyminumandcinnamomumzeylanicumessentialoilsagainstanophelesgambiaeandprospectsfortheiruseasbednettreatments AT clairedumenil electrophysiologicalandbehavioralcharacterizationofbioactivecompoundsofthethymusvulgariscymbopogonwinterianuscuminumcyminumandcinnamomumzeylanicumessentialoilsagainstanophelesgambiaeandprospectsfortheiruseasbednettreatments AT chantalmenut electrophysiologicalandbehavioralcharacterizationofbioactivecompoundsofthethymusvulgariscymbopogonwinterianuscuminumcyminumandcinnamomumzeylanicumessentialoilsagainstanophelesgambiaeandprospectsfortheiruseasbednettreatments AT thibaudmartin electrophysiologicalandbehavioralcharacterizationofbioactivecompoundsofthethymusvulgariscymbopogonwinterianuscuminumcyminumandcinnamomumzeylanicumessentialoilsagainstanophelesgambiaeandprospectsfortheiruseasbednettreatments |