Bazi Bushen Capsule Alleviates Post-Menopausal Atherosclerosis via GPER1-Dependent Anti-Inflammatory and Anti-Apoptotic Effects
Bazi Bushen capsule (BZBS), as a Chinese medicine used to relieve fatigue, has been proven effective for the treatment of atherogenesis through antilipid effects. To investigate the potential mechanism of BZBS in the anti-atherosclerotic effect, Ovx/ApoE-/- mice were applied to investigate the anti-...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-06-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2021.658998/full |
| _version_ | 1818882291847397376 |
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| author | Dan Huang Dan Huang Xindong Wang Xindong Wang Yunhong Zhu Yunhong Zhu Juexiao Gong Juexiao Gong Junqing Liang Yanfei Song Yiyan Zhang Yiyan Zhang Linsheng Liu Cong Wei |
| author_facet | Dan Huang Dan Huang Xindong Wang Xindong Wang Yunhong Zhu Yunhong Zhu Juexiao Gong Juexiao Gong Junqing Liang Yanfei Song Yiyan Zhang Yiyan Zhang Linsheng Liu Cong Wei |
| author_sort | Dan Huang |
| collection | DOAJ |
| description | Bazi Bushen capsule (BZBS), as a Chinese medicine used to relieve fatigue, has been proven effective for the treatment of atherogenesis through antilipid effects. To investigate the potential mechanism of BZBS in the anti-atherosclerotic effect, Ovx/ApoE-/- mice were applied to investigate the anti-atherosclerotic efficiency and potential mechanism of BZBS. Therapeutic effect was evaluated based on the number of CD68+ and CD3+ cells, the level of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1), and the ratio of cleaved caspase-3/caspase-3, as well as increasing ratio of Bcl2/Bax. Human umbilical vein endothelial cells (HUVECs) were chosen to evaluate the role of GPER1. Treatment with BZBS reduced lipid deposition by reducing the numbers of CD68+ and CD3+ cells, the level of ICAM-1 and VCAM-1, and the ratio of cleaved caspase-3/caspase-3, and increasing the ratio of Bcl2/Bax as compared with the control group. In si-GPER1-treated HUVECs, the anti-apoptotic effect of BZBS was decreased. This study revealed that BZBS exhibited a clear effect against atherogenesis via GPER1-dependent anti-inflammatory and anti-apoptotic mechanisms. We believe that this manuscript is informative and useful for researchers pursuing the related alleviation of post-menopausal AS via anti-inflammatory and anti-apoptotic mechanisms. |
| first_indexed | 2024-12-19T15:15:26Z |
| format | Article |
| id | doaj.art-8c78aa9c13154b61817c05769174ac59 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-19T15:15:26Z |
| publishDate | 2021-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-8c78aa9c13154b61817c05769174ac592022-12-21T20:16:09ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122021-06-011210.3389/fphar.2021.658998658998Bazi Bushen Capsule Alleviates Post-Menopausal Atherosclerosis via GPER1-Dependent Anti-Inflammatory and Anti-Apoptotic EffectsDan Huang0Dan Huang1Xindong Wang2Xindong Wang3Yunhong Zhu4Yunhong Zhu5Juexiao Gong6Juexiao Gong7Junqing Liang8Yanfei Song9Yiyan Zhang10Yiyan Zhang11Linsheng Liu12Cong Wei13Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, ChinaJiangsu Province Academy of Traditional Chinese Medicine, Nanjing, ChinaAffiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, ChinaJiangsu Province Academy of Traditional Chinese Medicine, Nanjing, ChinaAffiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, ChinaJiangsu Province Academy of Traditional Chinese Medicine, Nanjing, ChinaAffiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, ChinaJiangsu Province Academy of Traditional Chinese Medicine, Nanjing, ChinaNational Key Laboratory of Collateral Disease Research and Innovative Chinese Medicine, Shijiazhuang, ChinaNational Key Laboratory of Collateral Disease Research and Innovative Chinese Medicine, Shijiazhuang, ChinaAffiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, ChinaJiangsu Province Academy of Traditional Chinese Medicine, Nanjing, ChinaDepartment of Clinical Pharmacology, The First Affiliated Hospital of Soochow University, Suzhou, ChinaNational Key Laboratory of Collateral Disease Research and Innovative Chinese Medicine, Shijiazhuang, ChinaBazi Bushen capsule (BZBS), as a Chinese medicine used to relieve fatigue, has been proven effective for the treatment of atherogenesis through antilipid effects. To investigate the potential mechanism of BZBS in the anti-atherosclerotic effect, Ovx/ApoE-/- mice were applied to investigate the anti-atherosclerotic efficiency and potential mechanism of BZBS. Therapeutic effect was evaluated based on the number of CD68+ and CD3+ cells, the level of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1), and the ratio of cleaved caspase-3/caspase-3, as well as increasing ratio of Bcl2/Bax. Human umbilical vein endothelial cells (HUVECs) were chosen to evaluate the role of GPER1. Treatment with BZBS reduced lipid deposition by reducing the numbers of CD68+ and CD3+ cells, the level of ICAM-1 and VCAM-1, and the ratio of cleaved caspase-3/caspase-3, and increasing the ratio of Bcl2/Bax as compared with the control group. In si-GPER1-treated HUVECs, the anti-apoptotic effect of BZBS was decreased. This study revealed that BZBS exhibited a clear effect against atherogenesis via GPER1-dependent anti-inflammatory and anti-apoptotic mechanisms. We believe that this manuscript is informative and useful for researchers pursuing the related alleviation of post-menopausal AS via anti-inflammatory and anti-apoptotic mechanisms.https://www.frontiersin.org/articles/10.3389/fphar.2021.658998/fullatherosclerosisbazi bushen capsulelipid profileinflammationapoptosisGPER1 antagonist |
| spellingShingle | Dan Huang Dan Huang Xindong Wang Xindong Wang Yunhong Zhu Yunhong Zhu Juexiao Gong Juexiao Gong Junqing Liang Yanfei Song Yiyan Zhang Yiyan Zhang Linsheng Liu Cong Wei Bazi Bushen Capsule Alleviates Post-Menopausal Atherosclerosis via GPER1-Dependent Anti-Inflammatory and Anti-Apoptotic Effects Frontiers in Pharmacology atherosclerosis bazi bushen capsule lipid profile inflammation apoptosis GPER1 antagonist |
| title | Bazi Bushen Capsule Alleviates Post-Menopausal Atherosclerosis via GPER1-Dependent Anti-Inflammatory and Anti-Apoptotic Effects |
| title_full | Bazi Bushen Capsule Alleviates Post-Menopausal Atherosclerosis via GPER1-Dependent Anti-Inflammatory and Anti-Apoptotic Effects |
| title_fullStr | Bazi Bushen Capsule Alleviates Post-Menopausal Atherosclerosis via GPER1-Dependent Anti-Inflammatory and Anti-Apoptotic Effects |
| title_full_unstemmed | Bazi Bushen Capsule Alleviates Post-Menopausal Atherosclerosis via GPER1-Dependent Anti-Inflammatory and Anti-Apoptotic Effects |
| title_short | Bazi Bushen Capsule Alleviates Post-Menopausal Atherosclerosis via GPER1-Dependent Anti-Inflammatory and Anti-Apoptotic Effects |
| title_sort | bazi bushen capsule alleviates post menopausal atherosclerosis via gper1 dependent anti inflammatory and anti apoptotic effects |
| topic | atherosclerosis bazi bushen capsule lipid profile inflammation apoptosis GPER1 antagonist |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2021.658998/full |
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