Inflammation of the choroid plexus in progressive multiple sclerosis: accumulation of granulocytes and T cells
Abstract The choroid plexus (CP) is strategically located between the peripheral blood and the cerebrospinal fluid, and is involved in the regulation of central nervous system (CNS) homeostasis. In multiple sclerosis (MS), demyelination and inflammation occur in the CNS. While experimental animal mo...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2020-02-01
|
| Series: | Acta Neuropathologica Communications |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40478-020-0885-1 |
| _version_ | 1818676356223860736 |
|---|---|
| author | Sabela Rodríguez-Lorenzo Julia Konings Susanne van der Pol Alwin Kamermans Sandra Amor Jack van Horssen Maarten E. Witte Gijs Kooij Helga E. de Vries |
| author_facet | Sabela Rodríguez-Lorenzo Julia Konings Susanne van der Pol Alwin Kamermans Sandra Amor Jack van Horssen Maarten E. Witte Gijs Kooij Helga E. de Vries |
| author_sort | Sabela Rodríguez-Lorenzo |
| collection | DOAJ |
| description | Abstract The choroid plexus (CP) is strategically located between the peripheral blood and the cerebrospinal fluid, and is involved in the regulation of central nervous system (CNS) homeostasis. In multiple sclerosis (MS), demyelination and inflammation occur in the CNS. While experimental animal models of MS pointed to the CP as a key route for immune cell invasion of the CNS, little is known about the distribution of immune cells in the human CP during progressive phases of MS. Here, we use immunohistochemistry and confocal microscopy to explore the main immune cell populations in the CP of progressive MS patients and non-neuroinflammatory controls, in terms of abundance and location within the distinct CP compartments. We show for the first time that the CP stromal density of granulocytes and CD8+ T cells is higher in progressive MS patients compared to controls. In line with previous studies, the CP of both controls and progressive MS patients contains relatively high numbers of macrophages and dendritic cells. Moreover, we found virtually no B cells or plasma cells in the CP. MHCII+ antigen-presenting cells were often found in close proximity to T cells, suggesting constitutive CNS immune monitoring functions of the CP. Together, our data highlights the role of the CP in immune homeostasis and indicates the occurrence of mild inflammatory processes in the CP of progressive MS patients. However, our findings suggest that the CP is only marginally involved in immune cell migration into the CNS in chronic MS. |
| first_indexed | 2024-12-17T08:42:10Z |
| format | Article |
| id | doaj.art-8c7ddcfe2bec45a086daa724750ab49d |
| institution | Directory Open Access Journal |
| issn | 2051-5960 |
| language | English |
| last_indexed | 2024-12-17T08:42:10Z |
| publishDate | 2020-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Acta Neuropathologica Communications |
| spelling | doaj.art-8c7ddcfe2bec45a086daa724750ab49d2022-12-21T21:56:17ZengBMCActa Neuropathologica Communications2051-59602020-02-018111310.1186/s40478-020-0885-1Inflammation of the choroid plexus in progressive multiple sclerosis: accumulation of granulocytes and T cellsSabela Rodríguez-Lorenzo0Julia Konings1Susanne van der Pol2Alwin Kamermans3Sandra Amor4Jack van Horssen5Maarten E. Witte6Gijs Kooij7Helga E. de Vries8Department of Molecular Cell Biology and Immunology, MS Center Amsterdam, Amsterdam Neuroscience, AmsterdamUMC, Vrije Universiteit AmsterdamDepartment of Molecular Cell Biology and Immunology, MS Center Amsterdam, Amsterdam Neuroscience, AmsterdamUMC, Vrije Universiteit AmsterdamDepartment of Molecular Cell Biology and Immunology, MS Center Amsterdam, Amsterdam Neuroscience, AmsterdamUMC, Vrije Universiteit AmsterdamDepartment of Molecular Cell Biology and Immunology, MS Center Amsterdam, Amsterdam Neuroscience, AmsterdamUMC, Vrije Universiteit AmsterdamDepartment of Pathology, MS center Amsterdam, Amsterdam UMC, Vrije Universiteit AmsterdamDepartment of Molecular Cell Biology and Immunology, MS Center Amsterdam, Amsterdam Neuroscience, AmsterdamUMC, Vrije Universiteit AmsterdamDepartment of Molecular Cell Biology and Immunology, MS Center Amsterdam, Amsterdam Neuroscience, AmsterdamUMC, Vrije Universiteit AmsterdamDepartment of Molecular Cell Biology and Immunology, MS Center Amsterdam, Amsterdam Neuroscience, AmsterdamUMC, Vrije Universiteit AmsterdamDepartment of Molecular Cell Biology and Immunology, MS Center Amsterdam, Amsterdam Neuroscience, AmsterdamUMC, Vrije Universiteit AmsterdamAbstract The choroid plexus (CP) is strategically located between the peripheral blood and the cerebrospinal fluid, and is involved in the regulation of central nervous system (CNS) homeostasis. In multiple sclerosis (MS), demyelination and inflammation occur in the CNS. While experimental animal models of MS pointed to the CP as a key route for immune cell invasion of the CNS, little is known about the distribution of immune cells in the human CP during progressive phases of MS. Here, we use immunohistochemistry and confocal microscopy to explore the main immune cell populations in the CP of progressive MS patients and non-neuroinflammatory controls, in terms of abundance and location within the distinct CP compartments. We show for the first time that the CP stromal density of granulocytes and CD8+ T cells is higher in progressive MS patients compared to controls. In line with previous studies, the CP of both controls and progressive MS patients contains relatively high numbers of macrophages and dendritic cells. Moreover, we found virtually no B cells or plasma cells in the CP. MHCII+ antigen-presenting cells were often found in close proximity to T cells, suggesting constitutive CNS immune monitoring functions of the CP. Together, our data highlights the role of the CP in immune homeostasis and indicates the occurrence of mild inflammatory processes in the CP of progressive MS patients. However, our findings suggest that the CP is only marginally involved in immune cell migration into the CNS in chronic MS.https://doi.org/10.1186/s40478-020-0885-1Choroid plexusProgressive MSImmune cellsT cellsGranulocytesBlood-CSF barrier |
| spellingShingle | Sabela Rodríguez-Lorenzo Julia Konings Susanne van der Pol Alwin Kamermans Sandra Amor Jack van Horssen Maarten E. Witte Gijs Kooij Helga E. de Vries Inflammation of the choroid plexus in progressive multiple sclerosis: accumulation of granulocytes and T cells Acta Neuropathologica Communications Choroid plexus Progressive MS Immune cells T cells Granulocytes Blood-CSF barrier |
| title | Inflammation of the choroid plexus in progressive multiple sclerosis: accumulation of granulocytes and T cells |
| title_full | Inflammation of the choroid plexus in progressive multiple sclerosis: accumulation of granulocytes and T cells |
| title_fullStr | Inflammation of the choroid plexus in progressive multiple sclerosis: accumulation of granulocytes and T cells |
| title_full_unstemmed | Inflammation of the choroid plexus in progressive multiple sclerosis: accumulation of granulocytes and T cells |
| title_short | Inflammation of the choroid plexus in progressive multiple sclerosis: accumulation of granulocytes and T cells |
| title_sort | inflammation of the choroid plexus in progressive multiple sclerosis accumulation of granulocytes and t cells |
| topic | Choroid plexus Progressive MS Immune cells T cells Granulocytes Blood-CSF barrier |
| url | https://doi.org/10.1186/s40478-020-0885-1 |
| work_keys_str_mv | AT sabelarodriguezlorenzo inflammationofthechoroidplexusinprogressivemultiplesclerosisaccumulationofgranulocytesandtcells AT juliakonings inflammationofthechoroidplexusinprogressivemultiplesclerosisaccumulationofgranulocytesandtcells AT susannevanderpol inflammationofthechoroidplexusinprogressivemultiplesclerosisaccumulationofgranulocytesandtcells AT alwinkamermans inflammationofthechoroidplexusinprogressivemultiplesclerosisaccumulationofgranulocytesandtcells AT sandraamor inflammationofthechoroidplexusinprogressivemultiplesclerosisaccumulationofgranulocytesandtcells AT jackvanhorssen inflammationofthechoroidplexusinprogressivemultiplesclerosisaccumulationofgranulocytesandtcells AT maartenewitte inflammationofthechoroidplexusinprogressivemultiplesclerosisaccumulationofgranulocytesandtcells AT gijskooij inflammationofthechoroidplexusinprogressivemultiplesclerosisaccumulationofgranulocytesandtcells AT helgaedevries inflammationofthechoroidplexusinprogressivemultiplesclerosisaccumulationofgranulocytesandtcells |