Early Results of Autologous Cultivated Limbal Stem Cell Transplantation in Total Limbal Stem Cell Deficiency

Early Results of Autologous Cultivated Limbal Stem Cell Transplantation in Total Limbal Stem Cell Deficiency

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Main Authors: Mohammad Ali Javadi, Bahram Einollahi, Alireza Baradaran Rafiei, Hossein Baharvand, Marzieh Ebrahimi, Nasrin Rafati, Mojgan Rezaei Kanavi
Format: Article
Language:English
Published: Knowledge E 2008-12-01
Series:Journal of Ophthalmic & Vision Research
Online Access:http://www.jovr.ir/index.php/jovr/article/view/52
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author Mohammad Ali Javadi
Bahram Einollahi
Alireza Baradaran Rafiei
Hossein Baharvand
Marzieh Ebrahimi
Nasrin Rafati
Mojgan Rezaei Kanavi
author_facet Mohammad Ali Javadi
Bahram Einollahi
Alireza Baradaran Rafiei
Hossein Baharvand
Marzieh Ebrahimi
Nasrin Rafati
Mojgan Rezaei Kanavi
author_sort Mohammad Ali Javadi
collection DOAJ
description <!--[if gte mso 9]><xml> Normal 0 false false false MicrosoftInternetExplorer4 </xml><![endif]--><!--[if gte mso 9]><xml> </xml><![endif]--> <!--[if gte mso 10]> <style>
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 </style> <![endif]--> <p>PURPOSE: To report the early results of transplantation of autologous limbal stem cells cultivated on amniotic membrane (AM) in patients with total unilateral limbal stem cell deficiency (LSCD). METHODS: Four eyes of 4 patients with total unilateral LSCD confirmed with impression cytology underwent transplantation of autologous limbal stem cell cultivated on AM. At each follow up visit, a complete eye examination with special attention to recurrence or regression of vascularization, corneal opacification, and epithelial defect healing was performed. Digital imaging was performed at each follow up visit. Impression cytology was repeated in all cases after surgery. RESULTS: The patients were followed for 5-13 months. Visual acuity improved in all cases. Decrease in corneal opacification and vascularization was obvious in 3 cases with coverage of the cornea with corneal epithelium. Sectoral conjunctivalization was evident in these 3 cases, however the corneas were ready for transplantation. The procedure failed in one case with total corneal conjunctivalization. CONCLUSION: Transplantation of autologous stem cells cultivated on AM seems to be an effective way for total LSCD. More definite judgment needs longer follow up together with long-term results of corneal transplantation in these patients.</p> <!--[if gte mso 9]><xml> <w:WordDocument> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <w:PunctuationKerning /> <w:ValidateAgainstSchemas /> <w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid> <w:IgnoreMixedContent>false</w:IgnoreMixedContent> <w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText> <w:Compatibility> <w:BreakWrappedTables /> <w:SnapToGridInCell /> <w:WrapTextWithPunct /> <w:UseAsianBreakRules /> <w:DontGrowAutofit /> </w:Compatibility> <w:BrowserLevel>MicrosoftInternetExplorer4</w:BrowserLevel> </w:WordDocument> </xml><![endif]--><!--[if gte mso 9]><xml> <w:LatentStyles DefLockedState="false" LatentStyleCount="156"> </w:LatentStyles> </xml><![endif]--> <!-- /* Font Definitions */ @font-face {font-family:Palatino; mso-font-alt:"Book Antiqua"; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:7 0 0 0 147 0;} @font-face {font-family:Nazanin; mso-font-alt:"Courier New"; mso-font-charset:178; mso-generic-font-family:auto; mso-font-pitch:variable; mso-font-signature:24577 0 0 0 64 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; mso-bidi-font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:Nazanin;} @page Section1 {size:612.0pt 792.0pt; margin:72.0pt 90.0pt 72.0pt 90.0pt; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> <!--[if gte mso 10]> <style>
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spelling doaj.art-8c7e1fd7ee4742bd895fc95cc05cf9792022-12-22T04:34:22ZengKnowledge EJournal of Ophthalmic & Vision Research2008-20102008-322X2008-12-01127180Early Results of Autologous Cultivated Limbal Stem Cell Transplantation in Total Limbal Stem Cell DeficiencyMohammad Ali JavadiBahram EinollahiAlireza Baradaran RafieiHossein BaharvandMarzieh EbrahimiNasrin RafatiMojgan Rezaei Kanavi<!--[if gte mso 9]><xml> Normal 0 false false false MicrosoftInternetExplorer4 </xml><![endif]--><!--[if gte mso 9]><xml> </xml><![endif]--> <!--[if gte mso 10]> <style>
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 </style> <![endif]--> <p>PURPOSE: To report the early results of transplantation of autologous limbal stem cells cultivated on amniotic membrane (AM) in patients with total unilateral limbal stem cell deficiency (LSCD). METHODS: Four eyes of 4 patients with total unilateral LSCD confirmed with impression cytology underwent transplantation of autologous limbal stem cell cultivated on AM. At each follow up visit, a complete eye examination with special attention to recurrence or regression of vascularization, corneal opacification, and epithelial defect healing was performed. Digital imaging was performed at each follow up visit. Impression cytology was repeated in all cases after surgery. RESULTS: The patients were followed for 5-13 months. Visual acuity improved in all cases. Decrease in corneal opacification and vascularization was obvious in 3 cases with coverage of the cornea with corneal epithelium. Sectoral conjunctivalization was evident in these 3 cases, however the corneas were ready for transplantation. The procedure failed in one case with total corneal conjunctivalization. CONCLUSION: Transplantation of autologous stem cells cultivated on AM seems to be an effective way for total LSCD. More definite judgment needs longer follow up together with long-term results of corneal transplantation in these patients.</p> <!--[if gte mso 9]><xml> <w:WordDocument> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <w:PunctuationKerning /> <w:ValidateAgainstSchemas /> <w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid> <w:IgnoreMixedContent>false</w:IgnoreMixedContent> <w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText> <w:Compatibility> <w:BreakWrappedTables /> <w:SnapToGridInCell /> <w:WrapTextWithPunct /> <w:UseAsianBreakRules /> <w:DontGrowAutofit /> </w:Compatibility> <w:BrowserLevel>MicrosoftInternetExplorer4</w:BrowserLevel> </w:WordDocument> </xml><![endif]--><!--[if gte mso 9]><xml> <w:LatentStyles DefLockedState="false" LatentStyleCount="156"> </w:LatentStyles> </xml><![endif]--> <!-- /* Font Definitions */ @font-face {font-family:Palatino; mso-font-alt:"Book Antiqua"; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:7 0 0 0 147 0;} @font-face {font-family:Nazanin; mso-font-alt:"Courier New"; mso-font-charset:178; mso-generic-font-family:auto; mso-font-pitch:variable; mso-font-signature:24577 0 0 0 64 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; mso-bidi-font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:Nazanin;} @page Section1 {size:612.0pt 792.0pt; margin:72.0pt 90.0pt 72.0pt 90.0pt; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> <!--[if gte mso 10]> <style>
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spellingShingle Mohammad Ali Javadi
Bahram Einollahi
Alireza Baradaran Rafiei
Hossein Baharvand
Marzieh Ebrahimi
Nasrin Rafati
Mojgan Rezaei Kanavi
Early Results of Autologous Cultivated Limbal Stem Cell Transplantation in Total Limbal Stem Cell Deficiency
Journal of Ophthalmic & Vision Research
title Early Results of Autologous Cultivated Limbal Stem Cell Transplantation in Total Limbal Stem Cell Deficiency
title_full Early Results of Autologous Cultivated Limbal Stem Cell Transplantation in Total Limbal Stem Cell Deficiency
title_fullStr Early Results of Autologous Cultivated Limbal Stem Cell Transplantation in Total Limbal Stem Cell Deficiency
title_full_unstemmed Early Results of Autologous Cultivated Limbal Stem Cell Transplantation in Total Limbal Stem Cell Deficiency
title_short Early Results of Autologous Cultivated Limbal Stem Cell Transplantation in Total Limbal Stem Cell Deficiency
title_sort early results of autologous cultivated limbal stem cell transplantation in total limbal stem cell deficiency
url http://www.jovr.ir/index.php/jovr/article/view/52
work_keys_str_mv AT mohammadalijavadi earlyresultsofautologouscultivatedlimbalstemcelltransplantationintotallimbalstemcelldeficiency
AT bahrameinollahi earlyresultsofautologouscultivatedlimbalstemcelltransplantationintotallimbalstemcelldeficiency
AT alirezabaradaranrafiei earlyresultsofautologouscultivatedlimbalstemcelltransplantationintotallimbalstemcelldeficiency
AT hosseinbaharvand earlyresultsofautologouscultivatedlimbalstemcelltransplantationintotallimbalstemcelldeficiency
AT marziehebrahimi earlyresultsofautologouscultivatedlimbalstemcelltransplantationintotallimbalstemcelldeficiency
AT nasrinrafati earlyresultsofautologouscultivatedlimbalstemcelltransplantationintotallimbalstemcelldeficiency
AT mojganrezaeikanavi earlyresultsofautologouscultivatedlimbalstemcelltransplantationintotallimbalstemcelldeficiency

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