Control of levamisole residues in milk using a validated liquid chromatography-tandem mass spectrometry method
Concentrations of the anthelmintic agent levamisole were measured in a total of 85 raw cow milk samples collected during 2014 from dairy farms across Croatia. The liquid chromatographytandem mass spectrometry (LC-MS/MS) method used for levamisole quantification was validated according to the criteri...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Croatian Dairy Union
2016-03-01
|
| Series: | Mljekarstvo |
| Subjects: | |
| Online Access: | http://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=228074 |
| _version_ | 1818922028458049536 |
|---|---|
| author | Nina Bilandžić Đurđica Božić Luburić Božica Solomun Kolanović Ivana Varenina Ines Varga |
| author_facet | Nina Bilandžić Đurđica Božić Luburić Božica Solomun Kolanović Ivana Varenina Ines Varga |
| author_sort | Nina Bilandžić |
| collection | DOAJ |
| description | Concentrations of the anthelmintic agent levamisole were measured in a total of 85 raw cow milk samples collected during 2014 from dairy farms across Croatia. The liquid chromatographytandem mass spectrometry (LC-MS/MS) method used for levamisole quantification was validated according to the criteria set by Commission Decision 2002/657/EC. The following validation parameters were determined: limit of decision (CCα) 0.55 μg kg-1, detection of capability (CCβ) 0.59 μg kg-1, limit of detection (LOD) 0.06 μg kg-1, limit of quantification (LOQ) 0.22 μg kg-1, precision and accuracy (expressed as recovery) 97.3-100 %, intra-laboratory reproducibility (RSD) 4.2-5.6 %. Levamisole levels for 45 of the total of 85 samples were below the LOD value (0.06 µg kg-1). In the remaining 40 milk samples, levamisole was measured in the range from 0.061 to 0.142 µg kg-1 and the mean value was 0.092 µg kg-1. Accordingly, all concentrations analysed were below the LOQ value (0.22 µg kg-1) and limit of decision (CCα) of the method used. |
| first_indexed | 2024-12-20T01:47:02Z |
| format | Article |
| id | doaj.art-8c7ec6ab98b14c0c818498911eb5c121 |
| institution | Directory Open Access Journal |
| issn | 0026-704X 1846-4025 |
| language | English |
| last_indexed | 2024-12-20T01:47:02Z |
| publishDate | 2016-03-01 |
| publisher | Croatian Dairy Union |
| record_format | Article |
| series | Mljekarstvo |
| spelling | doaj.art-8c7ec6ab98b14c0c818498911eb5c1212022-12-21T19:57:43ZengCroatian Dairy UnionMljekarstvo0026-704X1846-40252016-03-0166214615310.15567/mljekarstvo.2016.0207Control of levamisole residues in milk using a validated liquid chromatography-tandem mass spectrometry methodNina Bilandžić0Đurđica Božić Luburić1Božica Solomun Kolanović2Ivana Varenina3Ines Varga4Laboratory for Residue Control, Department for Veterinary Public Health, Croatian Veterinary Institute, 10000 Zagreb, CroatiaLaboratory for Residue Control, Department for Veterinary Public Health, Croatian Veterinary Institute, 10000 Zagreb, CroatiaLaboratory for Residue Control, Department for Veterinary Public Health, Croatian Veterinary Institute, 10000 Zagreb, CroatiaLaboratory for Residue Control, Department for Veterinary Public Health, Croatian Veterinary Institute, 10000 Zagreb, CroatiaLaboratory for Residue Control, Department for Veterinary Public Health, Croatian Veterinary Institute, 10000 Zagreb, CroatiaConcentrations of the anthelmintic agent levamisole were measured in a total of 85 raw cow milk samples collected during 2014 from dairy farms across Croatia. The liquid chromatographytandem mass spectrometry (LC-MS/MS) method used for levamisole quantification was validated according to the criteria set by Commission Decision 2002/657/EC. The following validation parameters were determined: limit of decision (CCα) 0.55 μg kg-1, detection of capability (CCβ) 0.59 μg kg-1, limit of detection (LOD) 0.06 μg kg-1, limit of quantification (LOQ) 0.22 μg kg-1, precision and accuracy (expressed as recovery) 97.3-100 %, intra-laboratory reproducibility (RSD) 4.2-5.6 %. Levamisole levels for 45 of the total of 85 samples were below the LOD value (0.06 µg kg-1). In the remaining 40 milk samples, levamisole was measured in the range from 0.061 to 0.142 µg kg-1 and the mean value was 0.092 µg kg-1. Accordingly, all concentrations analysed were below the LOQ value (0.22 µg kg-1) and limit of decision (CCα) of the method used.http://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=228074levamisole; anthelmintic; milk; validation; LC-MS/MS |
| spellingShingle | Nina Bilandžić Đurđica Božić Luburić Božica Solomun Kolanović Ivana Varenina Ines Varga Control of levamisole residues in milk using a validated liquid chromatography-tandem mass spectrometry method Mljekarstvo levamisole; anthelmintic; milk; validation; LC-MS/MS |
| title | Control of levamisole residues in milk using a validated liquid chromatography-tandem mass spectrometry method |
| title_full | Control of levamisole residues in milk using a validated liquid chromatography-tandem mass spectrometry method |
| title_fullStr | Control of levamisole residues in milk using a validated liquid chromatography-tandem mass spectrometry method |
| title_full_unstemmed | Control of levamisole residues in milk using a validated liquid chromatography-tandem mass spectrometry method |
| title_short | Control of levamisole residues in milk using a validated liquid chromatography-tandem mass spectrometry method |
| title_sort | control of levamisole residues in milk using a validated liquid chromatography tandem mass spectrometry method |
| topic | levamisole; anthelmintic; milk; validation; LC-MS/MS |
| url | http://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=228074 |
| work_keys_str_mv | AT ninabilandzic controloflevamisoleresiduesinmilkusingavalidatedliquidchromatographytandemmassspectrometrymethod AT đurđicabozicluburic controloflevamisoleresiduesinmilkusingavalidatedliquidchromatographytandemmassspectrometrymethod AT bozicasolomunkolanovic controloflevamisoleresiduesinmilkusingavalidatedliquidchromatographytandemmassspectrometrymethod AT ivanavarenina controloflevamisoleresiduesinmilkusingavalidatedliquidchromatographytandemmassspectrometrymethod AT inesvarga controloflevamisoleresiduesinmilkusingavalidatedliquidchromatographytandemmassspectrometrymethod |