Chemical Profiling, Antiproliferative and Antimigratory Capacity of <i>Haberlea rhodopensis</i> Extracts in an In Vitro Platform of Various Human Cancer Cell Lines
<i>Haberlea rhodopensis</i> is a Balkan endemic plant that belongs to the <i>Gesneriaceae</i> family, and is believed to have medicinal use and health-promoting properties. This study aimed to (<b>i</b>) prepare aqueous (HAE) and ethanolic (HEE) extracts from the...
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| Format: | Article |
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MDPI AG
2022-11-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/12/2305 |
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| author | Katerina Spyridopoulou Sotiris Kyriakou Angeliki Nomikou Angelos Roupas Antreas Ermogenous Katerina Karamanoli Daniela Moyankova Dimitar Djilianov Alex Galanis Mihalis I. Panayiotidis Aglaia Pappa |
| author_facet | Katerina Spyridopoulou Sotiris Kyriakou Angeliki Nomikou Angelos Roupas Antreas Ermogenous Katerina Karamanoli Daniela Moyankova Dimitar Djilianov Alex Galanis Mihalis I. Panayiotidis Aglaia Pappa |
| author_sort | Katerina Spyridopoulou |
| collection | DOAJ |
| description | <i>Haberlea rhodopensis</i> is a Balkan endemic plant that belongs to the <i>Gesneriaceae</i> family, and is believed to have medicinal use and health-promoting properties. This study aimed to (<b>i</b>) prepare aqueous (HAE) and ethanolic (HEE) extracts from the leaves of <i>H. rhodopensis</i> from in vitro propagated plants, (<b>ii</b>) screen for their potential antiproliferative and antimigratory activities, and (<b>iii</b>) chemically characterize both HAE and HEE by identifying compounds which may contribute to their observed bioactivity thereby further supporting their potential use in biomedical applications. The antiproliferative activity of both extracts was assessed against six human cancer cell lines by employing the sulforhodamine-B (SRB) assay. HEE was found to be more potent in inhibiting cancer cell growth as compared to HAE. Therefore, HEE’s antimigratory effects were further studied in hepatocellular carcinoma (HepG2) and non-small cell lung adenocarcinoma (A459) cell lines as they were among the most sensitive ones to its antiproliferative activity. HEE was found to exert significant antimigratory concentration-dependent effects in both cell lines assessed with the wound healing assay. Chemical characterization by UPLC-MS/MS analysis identified that HEE contains higher levels of flavonoids, phenolic compounds, pigments (chlorophyll–/-b, lycopene, and β-carotene), monoterpenoids, and condensed tannins compared to HAE, while HAE, contains higher levels of soluble protein and sugars. Furthermore, HEE demonstrated remarkable antioxidant activity evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH<sup>●</sup>), 2,2-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS<sup>●+</sup>) and ferric reducing/antioxidant power (FRAP) assays. We have obtained comprehensive results highlighting the potential of HEE as a source of bioactive compounds with anticancer properties. Future studies should aim at identifying the chemical constituents responsible for the bioactivities observed, and focus on investigating HEE’s effects, in in vivo preclinical cancer models. |
| first_indexed | 2024-03-09T17:24:09Z |
| format | Article |
| id | doaj.art-8c7f71f7e7e84a1eab3cdd3a182a6883 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T17:24:09Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-8c7f71f7e7e84a1eab3cdd3a182a68832023-11-24T12:55:45ZengMDPI AGAntioxidants2076-39212022-11-011112230510.3390/antiox11122305Chemical Profiling, Antiproliferative and Antimigratory Capacity of <i>Haberlea rhodopensis</i> Extracts in an In Vitro Platform of Various Human Cancer Cell LinesKaterina Spyridopoulou0Sotiris Kyriakou1Angeliki Nomikou2Angelos Roupas3Antreas Ermogenous4Katerina Karamanoli5Daniela Moyankova6Dimitar Djilianov7Alex Galanis8Mihalis I. Panayiotidis9Aglaia Pappa10Department of Molecular Biology & Genetics, Faculty of Health Sciences, Democritus University of Thrace, 68100 Alexandroupolis, GreeceDepartment of Cancer Genetics, Therapeutics & Ultrastructural Pathology, The Cyprus Institute of Neurology & Genetics, Ayios Dometios, Nicosia 2371, CyprusDepartment of Molecular Biology & Genetics, Faculty of Health Sciences, Democritus University of Thrace, 68100 Alexandroupolis, GreeceDepartment of Molecular Biology & Genetics, Faculty of Health Sciences, Democritus University of Thrace, 68100 Alexandroupolis, GreeceDepartment of Molecular Biology & Genetics, Faculty of Health Sciences, Democritus University of Thrace, 68100 Alexandroupolis, GreeceLaboratory of Agricultural Chemistry, School of Agriculture, Faculty of Agriculture, Forestry and Natural Environment, Aristotle University Thessaloniki, 54124 Thessaloniki, GreeceDepartment of Functional Genetics, Abiotic and Biotic Stress, Agrobioinstitute, Agricultural Academy, 8 Dragan Tzankov Blvd., 1164 Sofia, BulgariaDepartment of Functional Genetics, Abiotic and Biotic Stress, Agrobioinstitute, Agricultural Academy, 8 Dragan Tzankov Blvd., 1164 Sofia, BulgariaDepartment of Molecular Biology & Genetics, Faculty of Health Sciences, Democritus University of Thrace, 68100 Alexandroupolis, GreeceDepartment of Cancer Genetics, Therapeutics & Ultrastructural Pathology, The Cyprus Institute of Neurology & Genetics, Ayios Dometios, Nicosia 2371, CyprusDepartment of Molecular Biology & Genetics, Faculty of Health Sciences, Democritus University of Thrace, 68100 Alexandroupolis, Greece<i>Haberlea rhodopensis</i> is a Balkan endemic plant that belongs to the <i>Gesneriaceae</i> family, and is believed to have medicinal use and health-promoting properties. This study aimed to (<b>i</b>) prepare aqueous (HAE) and ethanolic (HEE) extracts from the leaves of <i>H. rhodopensis</i> from in vitro propagated plants, (<b>ii</b>) screen for their potential antiproliferative and antimigratory activities, and (<b>iii</b>) chemically characterize both HAE and HEE by identifying compounds which may contribute to their observed bioactivity thereby further supporting their potential use in biomedical applications. The antiproliferative activity of both extracts was assessed against six human cancer cell lines by employing the sulforhodamine-B (SRB) assay. HEE was found to be more potent in inhibiting cancer cell growth as compared to HAE. Therefore, HEE’s antimigratory effects were further studied in hepatocellular carcinoma (HepG2) and non-small cell lung adenocarcinoma (A459) cell lines as they were among the most sensitive ones to its antiproliferative activity. HEE was found to exert significant antimigratory concentration-dependent effects in both cell lines assessed with the wound healing assay. Chemical characterization by UPLC-MS/MS analysis identified that HEE contains higher levels of flavonoids, phenolic compounds, pigments (chlorophyll–/-b, lycopene, and β-carotene), monoterpenoids, and condensed tannins compared to HAE, while HAE, contains higher levels of soluble protein and sugars. Furthermore, HEE demonstrated remarkable antioxidant activity evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH<sup>●</sup>), 2,2-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS<sup>●+</sup>) and ferric reducing/antioxidant power (FRAP) assays. We have obtained comprehensive results highlighting the potential of HEE as a source of bioactive compounds with anticancer properties. Future studies should aim at identifying the chemical constituents responsible for the bioactivities observed, and focus on investigating HEE’s effects, in in vivo preclinical cancer models.https://www.mdpi.com/2076-3921/11/12/2305<i>Haberlea rhodopensis</i>resurrection plantaqueous extractethanol extractantioxidant activityantimigratory activity |
| spellingShingle | Katerina Spyridopoulou Sotiris Kyriakou Angeliki Nomikou Angelos Roupas Antreas Ermogenous Katerina Karamanoli Daniela Moyankova Dimitar Djilianov Alex Galanis Mihalis I. Panayiotidis Aglaia Pappa Chemical Profiling, Antiproliferative and Antimigratory Capacity of <i>Haberlea rhodopensis</i> Extracts in an In Vitro Platform of Various Human Cancer Cell Lines Antioxidants <i>Haberlea rhodopensis</i> resurrection plant aqueous extract ethanol extract antioxidant activity antimigratory activity |
| title | Chemical Profiling, Antiproliferative and Antimigratory Capacity of <i>Haberlea rhodopensis</i> Extracts in an In Vitro Platform of Various Human Cancer Cell Lines |
| title_full | Chemical Profiling, Antiproliferative and Antimigratory Capacity of <i>Haberlea rhodopensis</i> Extracts in an In Vitro Platform of Various Human Cancer Cell Lines |
| title_fullStr | Chemical Profiling, Antiproliferative and Antimigratory Capacity of <i>Haberlea rhodopensis</i> Extracts in an In Vitro Platform of Various Human Cancer Cell Lines |
| title_full_unstemmed | Chemical Profiling, Antiproliferative and Antimigratory Capacity of <i>Haberlea rhodopensis</i> Extracts in an In Vitro Platform of Various Human Cancer Cell Lines |
| title_short | Chemical Profiling, Antiproliferative and Antimigratory Capacity of <i>Haberlea rhodopensis</i> Extracts in an In Vitro Platform of Various Human Cancer Cell Lines |
| title_sort | chemical profiling antiproliferative and antimigratory capacity of i haberlea rhodopensis i extracts in an in vitro platform of various human cancer cell lines |
| topic | <i>Haberlea rhodopensis</i> resurrection plant aqueous extract ethanol extract antioxidant activity antimigratory activity |
| url | https://www.mdpi.com/2076-3921/11/12/2305 |
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