Genetic Diversity Assessment of the International Maize and Wheat Improvement Center and Chinese Wheat Core Germplasms by Non-Denaturing Fluorescence In Situ Hybridization
Germplasm is the material basis for crop genetic improvement and related basic research. Knowledge of genetic diversity present in wheat is the prerequisite for wheat breeding and improvement. Non-denaturing fluorescence in situ hybridization (ND-FISH) is a powerful tool to distinguish chromosomal p...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/11/1403 |
| _version_ | 1827664347402338304 |
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| author | Manyu Yang Zujun Yang Wuyun Yang Ennian Yang |
| author_facet | Manyu Yang Zujun Yang Wuyun Yang Ennian Yang |
| author_sort | Manyu Yang |
| collection | DOAJ |
| description | Germplasm is the material basis for crop genetic improvement and related basic research. Knowledge of genetic diversity present in wheat is the prerequisite for wheat breeding and improvement. Non-denaturing fluorescence in situ hybridization (ND-FISH) is a powerful tool to distinguish chromosomal polymorphisms and evaluate genetic diversity in wheat. In this study, ND-FISH using Oligo-pSc119.2-1, Oligo-pTa535-1, and Oligo-(GAA)<sub>7</sub> as probes were used to analyze the genetic diversity among 60 International Maize and Wheat Improvement Center (CIMMYT) derived wheat lines, and 93 cultivated wheat and landraces from the Chinese wheat core germplasm. A total of 137 polymorphic FISH patterns were obtained, in which 41, 65, and 31 were from A-, B-, and D-genome chromosomes, respectively, indicating polymorphism of B-genome > A-genome > D-genome. In addition, 22 and 51 specific FISH types were observed in the two germplasm resource lines. Twelve types of rearrangements, including seven new translocations, were detected in all 153 wheat lines. Genetic relationships among 153 wheat lines were clustered into six groups. Our research provides cytological information for rational utilization of wheat germplasm resources. |
| first_indexed | 2024-03-10T00:58:49Z |
| format | Article |
| id | doaj.art-8c80581e426e437ebedecafb39149f3b |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T00:58:49Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-8c80581e426e437ebedecafb39149f3b2023-11-23T14:38:04ZengMDPI AGPlants2223-77472022-05-011111140310.3390/plants11111403Genetic Diversity Assessment of the International Maize and Wheat Improvement Center and Chinese Wheat Core Germplasms by Non-Denaturing Fluorescence In Situ HybridizationManyu Yang0Zujun Yang1Wuyun Yang2Ennian Yang3Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaSchool of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, ChinaCrop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaCrop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaGermplasm is the material basis for crop genetic improvement and related basic research. Knowledge of genetic diversity present in wheat is the prerequisite for wheat breeding and improvement. Non-denaturing fluorescence in situ hybridization (ND-FISH) is a powerful tool to distinguish chromosomal polymorphisms and evaluate genetic diversity in wheat. In this study, ND-FISH using Oligo-pSc119.2-1, Oligo-pTa535-1, and Oligo-(GAA)<sub>7</sub> as probes were used to analyze the genetic diversity among 60 International Maize and Wheat Improvement Center (CIMMYT) derived wheat lines, and 93 cultivated wheat and landraces from the Chinese wheat core germplasm. A total of 137 polymorphic FISH patterns were obtained, in which 41, 65, and 31 were from A-, B-, and D-genome chromosomes, respectively, indicating polymorphism of B-genome > A-genome > D-genome. In addition, 22 and 51 specific FISH types were observed in the two germplasm resource lines. Twelve types of rearrangements, including seven new translocations, were detected in all 153 wheat lines. Genetic relationships among 153 wheat lines were clustered into six groups. Our research provides cytological information for rational utilization of wheat germplasm resources.https://www.mdpi.com/2223-7747/11/11/1403wheatND-FISHgenetic diversitychromosomal translocation |
| spellingShingle | Manyu Yang Zujun Yang Wuyun Yang Ennian Yang Genetic Diversity Assessment of the International Maize and Wheat Improvement Center and Chinese Wheat Core Germplasms by Non-Denaturing Fluorescence In Situ Hybridization Plants wheat ND-FISH genetic diversity chromosomal translocation |
| title | Genetic Diversity Assessment of the International Maize and Wheat Improvement Center and Chinese Wheat Core Germplasms by Non-Denaturing Fluorescence In Situ Hybridization |
| title_full | Genetic Diversity Assessment of the International Maize and Wheat Improvement Center and Chinese Wheat Core Germplasms by Non-Denaturing Fluorescence In Situ Hybridization |
| title_fullStr | Genetic Diversity Assessment of the International Maize and Wheat Improvement Center and Chinese Wheat Core Germplasms by Non-Denaturing Fluorescence In Situ Hybridization |
| title_full_unstemmed | Genetic Diversity Assessment of the International Maize and Wheat Improvement Center and Chinese Wheat Core Germplasms by Non-Denaturing Fluorescence In Situ Hybridization |
| title_short | Genetic Diversity Assessment of the International Maize and Wheat Improvement Center and Chinese Wheat Core Germplasms by Non-Denaturing Fluorescence In Situ Hybridization |
| title_sort | genetic diversity assessment of the international maize and wheat improvement center and chinese wheat core germplasms by non denaturing fluorescence in situ hybridization |
| topic | wheat ND-FISH genetic diversity chromosomal translocation |
| url | https://www.mdpi.com/2223-7747/11/11/1403 |
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