N-Doped Reduced Graphene Oxide/Gold Nanoparticles Composite as an Improved Sensing Platform for Simultaneous Detection of Dopamine, Ascorbic Acid, and Uric Acid
Gold nanoparticles (AuNPs) were homogeneously electrodeposited on nitrogen-doped reduced graphene oxide (N-rGO) to modify a glassy carbon electrode (GCE/N-rGO-Au) in order to improve the simultaneous detection of dopamine (DA), ascorbic acid (AA), and uric acid (UA). N-rGO was prepared by the hydrot...
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MDPI AG
2020-08-01
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| author | Daria Minta Zoraida González Piotr Wiench Stanisław Gryglewicz Grażyna Gryglewicz |
| author_facet | Daria Minta Zoraida González Piotr Wiench Stanisław Gryglewicz Grażyna Gryglewicz |
| author_sort | Daria Minta |
| collection | DOAJ |
| description | Gold nanoparticles (AuNPs) were homogeneously electrodeposited on nitrogen-doped reduced graphene oxide (N-rGO) to modify a glassy carbon electrode (GCE/N-rGO-Au) in order to improve the simultaneous detection of dopamine (DA), ascorbic acid (AA), and uric acid (UA). N-rGO was prepared by the hydrothermal treatment of graphene oxide (GO) and urea at 180 °C for 12 h. AuNPs were subsequently electrodeposited onto the surface of GCE/N-rGO using 1 mM HAuCl<sub>4</sub> solution. The morphology and chemical composition of the synthesized materials were characterized by field-emission scanning electron microscopy and X-ray photoelectron spectroscopy. The electrochemical performance of the modified electrodes was investigated through cyclic voltammetry and differential pulse voltammetry measurements. Compared to GCE/rGO-Au, GCE/N-rGO-Au exhibited better electrochemical performance towards the simultaneous detection of the three analytes due to the more homogeneous distribution of the metallic nanoparticles as a result of more efficient anchoring on the N-doped areas of the graphene structure. The GCE/N-rGO-Au-based sensor operated in a wide linear range of DA (3–100 µM), AA (550–1500 µM), and UA (20–1000 µM) concentrations with a detection limit of 2.4, 58, and 8.7 µM, respectively, and exhibited satisfactory peak potential separation values of 0.34 V (AA-DA), 0.20 V, (DA-UA) and 0.54 V (AA-UA). Remarkably, GCE/N-rGO-Au showed a very low detection limit of 385 nM towards DA, not being susceptible to interference, and maintained 90% of its initial electrochemical signal after one month, indicating an excellent long-term stability. |
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| spelling | doaj.art-8c806abae64d4628bf8147e943f049e82023-11-20T09:29:02ZengMDPI AGSensors1424-82202020-08-012016442710.3390/s20164427N-Doped Reduced Graphene Oxide/Gold Nanoparticles Composite as an Improved Sensing Platform for Simultaneous Detection of Dopamine, Ascorbic Acid, and Uric AcidDaria Minta0Zoraida González1Piotr Wiench2Stanisław Gryglewicz3Grażyna Gryglewicz4Department of Process Engineering and Technology of Polymer and Carbon Materials, Faculty of Chemistry, Wrocław University of Science and Technology, Gdańska 7/9, 50-344 Wrocław, PolandInstituto de Ciencia y Tecnología del Carbono, INCAR-CSIC, Francisco Pintado Fe 26, 33011 Oviedo, SpainDepartment of Process Engineering and Technology of Polymer and Carbon Materials, Faculty of Chemistry, Wrocław University of Science and Technology, Gdańska 7/9, 50-344 Wrocław, PolandDepartment of Engineering and Technology of Chemical Processes, Faculty of Chemistry, Wrocław University of Science and Technology, Smoluchowskiego 25, 50-372 Wrocław, PolandDepartment of Process Engineering and Technology of Polymer and Carbon Materials, Faculty of Chemistry, Wrocław University of Science and Technology, Gdańska 7/9, 50-344 Wrocław, PolandGold nanoparticles (AuNPs) were homogeneously electrodeposited on nitrogen-doped reduced graphene oxide (N-rGO) to modify a glassy carbon electrode (GCE/N-rGO-Au) in order to improve the simultaneous detection of dopamine (DA), ascorbic acid (AA), and uric acid (UA). N-rGO was prepared by the hydrothermal treatment of graphene oxide (GO) and urea at 180 °C for 12 h. AuNPs were subsequently electrodeposited onto the surface of GCE/N-rGO using 1 mM HAuCl<sub>4</sub> solution. The morphology and chemical composition of the synthesized materials were characterized by field-emission scanning electron microscopy and X-ray photoelectron spectroscopy. The electrochemical performance of the modified electrodes was investigated through cyclic voltammetry and differential pulse voltammetry measurements. Compared to GCE/rGO-Au, GCE/N-rGO-Au exhibited better electrochemical performance towards the simultaneous detection of the three analytes due to the more homogeneous distribution of the metallic nanoparticles as a result of more efficient anchoring on the N-doped areas of the graphene structure. The GCE/N-rGO-Au-based sensor operated in a wide linear range of DA (3–100 µM), AA (550–1500 µM), and UA (20–1000 µM) concentrations with a detection limit of 2.4, 58, and 8.7 µM, respectively, and exhibited satisfactory peak potential separation values of 0.34 V (AA-DA), 0.20 V, (DA-UA) and 0.54 V (AA-UA). Remarkably, GCE/N-rGO-Au showed a very low detection limit of 385 nM towards DA, not being susceptible to interference, and maintained 90% of its initial electrochemical signal after one month, indicating an excellent long-term stability.https://www.mdpi.com/1424-8220/20/16/4427electrochemical sensorsimultaneous detectiondopamineinterferencenitrogen-doped reduced graphene oxidegold nanoparticles |
| spellingShingle | Daria Minta Zoraida González Piotr Wiench Stanisław Gryglewicz Grażyna Gryglewicz N-Doped Reduced Graphene Oxide/Gold Nanoparticles Composite as an Improved Sensing Platform for Simultaneous Detection of Dopamine, Ascorbic Acid, and Uric Acid Sensors electrochemical sensor simultaneous detection dopamine interference nitrogen-doped reduced graphene oxide gold nanoparticles |
| title | N-Doped Reduced Graphene Oxide/Gold Nanoparticles Composite as an Improved Sensing Platform for Simultaneous Detection of Dopamine, Ascorbic Acid, and Uric Acid |
| title_full | N-Doped Reduced Graphene Oxide/Gold Nanoparticles Composite as an Improved Sensing Platform for Simultaneous Detection of Dopamine, Ascorbic Acid, and Uric Acid |
| title_fullStr | N-Doped Reduced Graphene Oxide/Gold Nanoparticles Composite as an Improved Sensing Platform for Simultaneous Detection of Dopamine, Ascorbic Acid, and Uric Acid |
| title_full_unstemmed | N-Doped Reduced Graphene Oxide/Gold Nanoparticles Composite as an Improved Sensing Platform for Simultaneous Detection of Dopamine, Ascorbic Acid, and Uric Acid |
| title_short | N-Doped Reduced Graphene Oxide/Gold Nanoparticles Composite as an Improved Sensing Platform for Simultaneous Detection of Dopamine, Ascorbic Acid, and Uric Acid |
| title_sort | n doped reduced graphene oxide gold nanoparticles composite as an improved sensing platform for simultaneous detection of dopamine ascorbic acid and uric acid |
| topic | electrochemical sensor simultaneous detection dopamine interference nitrogen-doped reduced graphene oxide gold nanoparticles |
| url | https://www.mdpi.com/1424-8220/20/16/4427 |
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