Offspring from mouse embryos developed using a simple incubator-free culture system with a deoxidizing agent.
To culture preimplantation embryos in vitro, water-jacketed CO(2) incubators are used widely for maintaining an optimal culture environment in terms of gas phase, temperature and humidity. We investigated the possibility of mouse embryo culture in a plastic bag kept at 37°C. Zygotes derived from in...
Main Authors: | Fumiaki Itoi, Mikiko Tokoro, Yukari Terashita, Kazuo Yamagata, Noritaka Fukunaga, Yoshimasa Asada, Teruhiko Wakayama |
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Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2012-01-01
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Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC3467247?pdf=render |
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