Identification of Putative Genes Involved in Limonoids Biosynthesis in Citrus by Comparative Transcriptomic Analysis
Limonoids produced by citrus are a group of highly bioactive secondary metabolites which provide health benefits for humans. Currently there is a lack of information derived from research on the genetic mechanisms controlling the biosynthesis of limonoids, which has limited the improvement of citrus...
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Frontiers Media S.A.
2017-05-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2017.00782/full |
| _version_ | 1819230709530755072 |
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| author | Fusheng Wang Fusheng Wang Fusheng Wang Mei Wang Xiaona Liu Yuanyuan Xu Shiping Zhu Shiping Zhu Wanxia Shen Wanxia Shen Xiaochun Zhao Xiaochun Zhao |
| author_facet | Fusheng Wang Fusheng Wang Fusheng Wang Mei Wang Xiaona Liu Yuanyuan Xu Shiping Zhu Shiping Zhu Wanxia Shen Wanxia Shen Xiaochun Zhao Xiaochun Zhao |
| author_sort | Fusheng Wang |
| collection | DOAJ |
| description | Limonoids produced by citrus are a group of highly bioactive secondary metabolites which provide health benefits for humans. Currently there is a lack of information derived from research on the genetic mechanisms controlling the biosynthesis of limonoids, which has limited the improvement of citrus for high production of limonoids. In this study, the transcriptome sequences of leaves, phloems and seeds of pummelo (Citrus grandis (L.) Osbeck) at different development stages with variances in limonoids contents were used for digital gene expression profiling analysis in order to identify the genes corresponding to the biosynthesis of limonoids. Pair-wise comparison of transcriptional profiles between different tissues identified 924 differentially expressed genes commonly shared between them. Expression pattern analysis suggested that 382 genes from three conjunctive groups of K-means clustering could be possibly related to the biosynthesis of limonoids. Correlation analysis with the samples from different genotypes, and different developing tissues of the citrus revealed that the expression of 15 candidate genes were highly correlated with the contents of limonoids. Among them, the cytochrome P450s (CYP450s) and transcriptional factor MYB demonstrated significantly high correlation coefficients, which indicated the importance of those genes on the biosynthesis of limonoids. CiOSC gene encoding the critical enzyme oxidosqualene cyclase (OSC) for biosynthesis of the precursor of triterpene scaffolds was found positively corresponding to the accumulation of limonoids during the development of seeds. Suppressing the expression of CiOSC with VIGS (Virus-induced gene silencing) demonstrated that the level of gene silencing was significantly correlated to the reduction of limonoids contents. The results indicated that the CiOSC gene plays a pivotal role in biosynthesis of limonoids. |
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| language | English |
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| publishDate | 2017-05-01 |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-8c821729660b4469a12d2e369901b9b12022-12-21T17:48:43ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-05-01810.3389/fpls.2017.00782259707Identification of Putative Genes Involved in Limonoids Biosynthesis in Citrus by Comparative Transcriptomic AnalysisFusheng Wang0Fusheng Wang1Fusheng Wang2Mei Wang3Xiaona Liu4Yuanyuan Xu5Shiping Zhu6Shiping Zhu7Wanxia Shen8Wanxia Shen9Xiaochun Zhao10Xiaochun Zhao11Citrus Research Institute, Southwest University and Chinese Academy of Agricultural SciencesChongqing, ChinaNational Citrus Engineering Research CenterChongqing, ChinaCollege of Horticulture and Landscape Architecture, Southwest UniversityChongqing, ChinaChongqing Yongchuan Institute for Food and Drug ControlChongqing, ChinaCitrus Research Institute, Southwest University and Chinese Academy of Agricultural SciencesChongqing, ChinaCitrus Research Institute, Southwest University and Chinese Academy of Agricultural SciencesChongqing, ChinaCitrus Research Institute, Southwest University and Chinese Academy of Agricultural SciencesChongqing, ChinaNational Citrus Engineering Research CenterChongqing, ChinaCitrus Research Institute, Southwest University and Chinese Academy of Agricultural SciencesChongqing, ChinaNational Citrus Engineering Research CenterChongqing, ChinaCitrus Research Institute, Southwest University and Chinese Academy of Agricultural SciencesChongqing, ChinaNational Citrus Engineering Research CenterChongqing, ChinaLimonoids produced by citrus are a group of highly bioactive secondary metabolites which provide health benefits for humans. Currently there is a lack of information derived from research on the genetic mechanisms controlling the biosynthesis of limonoids, which has limited the improvement of citrus for high production of limonoids. In this study, the transcriptome sequences of leaves, phloems and seeds of pummelo (Citrus grandis (L.) Osbeck) at different development stages with variances in limonoids contents were used for digital gene expression profiling analysis in order to identify the genes corresponding to the biosynthesis of limonoids. Pair-wise comparison of transcriptional profiles between different tissues identified 924 differentially expressed genes commonly shared between them. Expression pattern analysis suggested that 382 genes from three conjunctive groups of K-means clustering could be possibly related to the biosynthesis of limonoids. Correlation analysis with the samples from different genotypes, and different developing tissues of the citrus revealed that the expression of 15 candidate genes were highly correlated with the contents of limonoids. Among them, the cytochrome P450s (CYP450s) and transcriptional factor MYB demonstrated significantly high correlation coefficients, which indicated the importance of those genes on the biosynthesis of limonoids. CiOSC gene encoding the critical enzyme oxidosqualene cyclase (OSC) for biosynthesis of the precursor of triterpene scaffolds was found positively corresponding to the accumulation of limonoids during the development of seeds. Suppressing the expression of CiOSC with VIGS (Virus-induced gene silencing) demonstrated that the level of gene silencing was significantly correlated to the reduction of limonoids contents. The results indicated that the CiOSC gene plays a pivotal role in biosynthesis of limonoids.http://journal.frontiersin.org/article/10.3389/fpls.2017.00782/fullbiosynthesis of limonoidstranscriptomedigital gene expression profilingVIGSCiOSC |
| spellingShingle | Fusheng Wang Fusheng Wang Fusheng Wang Mei Wang Xiaona Liu Yuanyuan Xu Shiping Zhu Shiping Zhu Wanxia Shen Wanxia Shen Xiaochun Zhao Xiaochun Zhao Identification of Putative Genes Involved in Limonoids Biosynthesis in Citrus by Comparative Transcriptomic Analysis Frontiers in Plant Science biosynthesis of limonoids transcriptome digital gene expression profiling VIGS CiOSC |
| title | Identification of Putative Genes Involved in Limonoids Biosynthesis in Citrus by Comparative Transcriptomic Analysis |
| title_full | Identification of Putative Genes Involved in Limonoids Biosynthesis in Citrus by Comparative Transcriptomic Analysis |
| title_fullStr | Identification of Putative Genes Involved in Limonoids Biosynthesis in Citrus by Comparative Transcriptomic Analysis |
| title_full_unstemmed | Identification of Putative Genes Involved in Limonoids Biosynthesis in Citrus by Comparative Transcriptomic Analysis |
| title_short | Identification of Putative Genes Involved in Limonoids Biosynthesis in Citrus by Comparative Transcriptomic Analysis |
| title_sort | identification of putative genes involved in limonoids biosynthesis in citrus by comparative transcriptomic analysis |
| topic | biosynthesis of limonoids transcriptome digital gene expression profiling VIGS CiOSC |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2017.00782/full |
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