3-Ketoacyl-ACP synthase III FabH1 is essential for branched-chain DSF family signals in Xanthomonas oryzae pv. oryzae
Abstract The 3-ketoacyl-ACP synthase III (FabH), a key enzyme for bacteria growth, catalyses the last step of the initiation of bacterial fatty acid synthesis. Rice bacterial blight is caused by the pathogen Xanthomonas oryzae pv. oryzae (Xoo), which is widely studied as a model bacterium. Bioinform...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-06-01
|
| Series: | Phytopathology Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s42483-023-00180-2 |
| _version_ | 1797801580932628480 |
|---|---|
| author | Mingfeng Yan Yonghong Yu Lizhen Luo Mei Huang Yuanyin Zhang Jingtong Su Wenbin Zhang Jincheng Ma Zhe Hu Haihong Wang |
| author_facet | Mingfeng Yan Yonghong Yu Lizhen Luo Mei Huang Yuanyin Zhang Jingtong Su Wenbin Zhang Jincheng Ma Zhe Hu Haihong Wang |
| author_sort | Mingfeng Yan |
| collection | DOAJ |
| description | Abstract The 3-ketoacyl-ACP synthase III (FabH), a key enzyme for bacteria growth, catalyses the last step of the initiation of bacterial fatty acid synthesis. Rice bacterial blight is caused by the pathogen Xanthomonas oryzae pv. oryzae (Xoo), which is widely studied as a model bacterium. Bioinformatics analysis showed that the X. oryzae pv. oryzae PXO99A genome encodes three FabH homologous proteins with unknown functions. In this study, we found that only PXO_02706 (fabH1) encodes a functional FabH, the key enzyme in the production of branched-chain fatty acid, which is essential for the branched-chain diffusible signal factor family signals in Xoo. Interestingly, we found that FabH1 is not essential for fatty acid biosynthesis in Xoo. Pathogenicity analysis showed that loss of fabH1 caused a significant decrease in virulence of Xoo. Genetic and phenotypic analyses revealed that fabH1 plays a key role in multiple Xoo virulence-related activities, including exopolysaccharide (EPS) production, biofilm formation, motility, and resistance to environmental stresses. |
| first_indexed | 2024-03-13T04:52:22Z |
| format | Article |
| id | doaj.art-8c828d55be9d49758a3f17961a733189 |
| institution | Directory Open Access Journal |
| issn | 2524-4167 |
| language | English |
| last_indexed | 2024-03-13T04:52:22Z |
| publishDate | 2023-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Phytopathology Research |
| spelling | doaj.art-8c828d55be9d49758a3f17961a7331892023-06-18T11:08:10ZengBMCPhytopathology Research2524-41672023-06-015111210.1186/s42483-023-00180-23-Ketoacyl-ACP synthase III FabH1 is essential for branched-chain DSF family signals in Xanthomonas oryzae pv. oryzaeMingfeng Yan0Yonghong Yu1Lizhen Luo2Mei Huang3Yuanyin Zhang4Jingtong Su5Wenbin Zhang6Jincheng Ma7Zhe Hu8Haihong Wang9Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural UniversityGuangdong Food and Drug Vocational CollegeGuangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural UniversityGuangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural UniversityGuangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural UniversityGuangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural UniversityGuangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural UniversityGuangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural UniversityGuangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural UniversityGuangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural UniversityAbstract The 3-ketoacyl-ACP synthase III (FabH), a key enzyme for bacteria growth, catalyses the last step of the initiation of bacterial fatty acid synthesis. Rice bacterial blight is caused by the pathogen Xanthomonas oryzae pv. oryzae (Xoo), which is widely studied as a model bacterium. Bioinformatics analysis showed that the X. oryzae pv. oryzae PXO99A genome encodes three FabH homologous proteins with unknown functions. In this study, we found that only PXO_02706 (fabH1) encodes a functional FabH, the key enzyme in the production of branched-chain fatty acid, which is essential for the branched-chain diffusible signal factor family signals in Xoo. Interestingly, we found that FabH1 is not essential for fatty acid biosynthesis in Xoo. Pathogenicity analysis showed that loss of fabH1 caused a significant decrease in virulence of Xoo. Genetic and phenotypic analyses revealed that fabH1 plays a key role in multiple Xoo virulence-related activities, including exopolysaccharide (EPS) production, biofilm formation, motility, and resistance to environmental stresses.https://doi.org/10.1186/s42483-023-00180-2Xanthomonas oryzae pv. oryzae3-Ketoacyl-ACP synthases IIIFatty acid biosynthesisBranched-chain DSF-family signalsPathogenesis |
| spellingShingle | Mingfeng Yan Yonghong Yu Lizhen Luo Mei Huang Yuanyin Zhang Jingtong Su Wenbin Zhang Jincheng Ma Zhe Hu Haihong Wang 3-Ketoacyl-ACP synthase III FabH1 is essential for branched-chain DSF family signals in Xanthomonas oryzae pv. oryzae Phytopathology Research Xanthomonas oryzae pv. oryzae 3-Ketoacyl-ACP synthases III Fatty acid biosynthesis Branched-chain DSF-family signals Pathogenesis |
| title | 3-Ketoacyl-ACP synthase III FabH1 is essential for branched-chain DSF family signals in Xanthomonas oryzae pv. oryzae |
| title_full | 3-Ketoacyl-ACP synthase III FabH1 is essential for branched-chain DSF family signals in Xanthomonas oryzae pv. oryzae |
| title_fullStr | 3-Ketoacyl-ACP synthase III FabH1 is essential for branched-chain DSF family signals in Xanthomonas oryzae pv. oryzae |
| title_full_unstemmed | 3-Ketoacyl-ACP synthase III FabH1 is essential for branched-chain DSF family signals in Xanthomonas oryzae pv. oryzae |
| title_short | 3-Ketoacyl-ACP synthase III FabH1 is essential for branched-chain DSF family signals in Xanthomonas oryzae pv. oryzae |
| title_sort | 3 ketoacyl acp synthase iii fabh1 is essential for branched chain dsf family signals in xanthomonas oryzae pv oryzae |
| topic | Xanthomonas oryzae pv. oryzae 3-Ketoacyl-ACP synthases III Fatty acid biosynthesis Branched-chain DSF-family signals Pathogenesis |
| url | https://doi.org/10.1186/s42483-023-00180-2 |
| work_keys_str_mv | AT mingfengyan 3ketoacylacpsynthaseiiifabh1isessentialforbranchedchaindsffamilysignalsinxanthomonasoryzaepvoryzae AT yonghongyu 3ketoacylacpsynthaseiiifabh1isessentialforbranchedchaindsffamilysignalsinxanthomonasoryzaepvoryzae AT lizhenluo 3ketoacylacpsynthaseiiifabh1isessentialforbranchedchaindsffamilysignalsinxanthomonasoryzaepvoryzae AT meihuang 3ketoacylacpsynthaseiiifabh1isessentialforbranchedchaindsffamilysignalsinxanthomonasoryzaepvoryzae AT yuanyinzhang 3ketoacylacpsynthaseiiifabh1isessentialforbranchedchaindsffamilysignalsinxanthomonasoryzaepvoryzae AT jingtongsu 3ketoacylacpsynthaseiiifabh1isessentialforbranchedchaindsffamilysignalsinxanthomonasoryzaepvoryzae AT wenbinzhang 3ketoacylacpsynthaseiiifabh1isessentialforbranchedchaindsffamilysignalsinxanthomonasoryzaepvoryzae AT jinchengma 3ketoacylacpsynthaseiiifabh1isessentialforbranchedchaindsffamilysignalsinxanthomonasoryzaepvoryzae AT zhehu 3ketoacylacpsynthaseiiifabh1isessentialforbranchedchaindsffamilysignalsinxanthomonasoryzaepvoryzae AT haihongwang 3ketoacylacpsynthaseiiifabh1isessentialforbranchedchaindsffamilysignalsinxanthomonasoryzaepvoryzae |