The Integrity of the HMR complex is necessary for centromeric binding and reproductive isolation in Drosophila.
Postzygotic isolation by genomic conflict is a major cause for the formation of species. Despite its importance, the molecular mechanisms that result in the lethality of interspecies hybrids are still largely unclear. The genus Drosophila, which contains over 1600 different species, is one of the be...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2021-08-01
|
| Series: | PLoS Genetics |
| Online Access: | https://doi.org/10.1371/journal.pgen.1009744 |
| _version_ | 1811191564007899136 |
|---|---|
| author | Andrea Lukacs Andreas W Thomae Peter Krueger Tamas Schauer Anuroop V Venkatasubramani Natalia Y Kochanova Wasim Aftab Rupam Choudhury Ignasi Forne Axel Imhof |
| author_facet | Andrea Lukacs Andreas W Thomae Peter Krueger Tamas Schauer Anuroop V Venkatasubramani Natalia Y Kochanova Wasim Aftab Rupam Choudhury Ignasi Forne Axel Imhof |
| author_sort | Andrea Lukacs |
| collection | DOAJ |
| description | Postzygotic isolation by genomic conflict is a major cause for the formation of species. Despite its importance, the molecular mechanisms that result in the lethality of interspecies hybrids are still largely unclear. The genus Drosophila, which contains over 1600 different species, is one of the best characterized model systems to study these questions. We showed in the past that the expression levels of the two hybrid incompatibility factors Hmr and Lhr diverged in the two closely related Drosophila species, D. melanogaster and D. simulans, resulting in an increased level of both proteins in interspecies hybrids. The overexpression of the two proteins also leads to mitotic defects, a misregulation in the expression of transposable elements and decreased fertility in pure species. In this work, we describe a distinct six subunit protein complex containing HMR and LHR and analyse the effect of Hmr mutations on complex integrity and function. Our experiments suggest that HMR needs to bring together components of centromeric and pericentromeric chromatin to fulfil its physiological function and to cause hybrid male lethality. |
| first_indexed | 2024-04-11T20:16:11Z |
| format | Article |
| id | doaj.art-8c8556f9eaf742c38d19bf03b185c7dd |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-04-11T20:16:11Z |
| publishDate | 2021-08-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-8c8556f9eaf742c38d19bf03b185c7dd2022-12-22T04:04:57ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042021-08-01178e100974410.1371/journal.pgen.1009744The Integrity of the HMR complex is necessary for centromeric binding and reproductive isolation in Drosophila.Andrea LukacsAndreas W ThomaePeter KruegerTamas SchauerAnuroop V VenkatasubramaniNatalia Y KochanovaWasim AftabRupam ChoudhuryIgnasi ForneAxel ImhofPostzygotic isolation by genomic conflict is a major cause for the formation of species. Despite its importance, the molecular mechanisms that result in the lethality of interspecies hybrids are still largely unclear. The genus Drosophila, which contains over 1600 different species, is one of the best characterized model systems to study these questions. We showed in the past that the expression levels of the two hybrid incompatibility factors Hmr and Lhr diverged in the two closely related Drosophila species, D. melanogaster and D. simulans, resulting in an increased level of both proteins in interspecies hybrids. The overexpression of the two proteins also leads to mitotic defects, a misregulation in the expression of transposable elements and decreased fertility in pure species. In this work, we describe a distinct six subunit protein complex containing HMR and LHR and analyse the effect of Hmr mutations on complex integrity and function. Our experiments suggest that HMR needs to bring together components of centromeric and pericentromeric chromatin to fulfil its physiological function and to cause hybrid male lethality.https://doi.org/10.1371/journal.pgen.1009744 |
| spellingShingle | Andrea Lukacs Andreas W Thomae Peter Krueger Tamas Schauer Anuroop V Venkatasubramani Natalia Y Kochanova Wasim Aftab Rupam Choudhury Ignasi Forne Axel Imhof The Integrity of the HMR complex is necessary for centromeric binding and reproductive isolation in Drosophila. PLoS Genetics |
| title | The Integrity of the HMR complex is necessary for centromeric binding and reproductive isolation in Drosophila. |
| title_full | The Integrity of the HMR complex is necessary for centromeric binding and reproductive isolation in Drosophila. |
| title_fullStr | The Integrity of the HMR complex is necessary for centromeric binding and reproductive isolation in Drosophila. |
| title_full_unstemmed | The Integrity of the HMR complex is necessary for centromeric binding and reproductive isolation in Drosophila. |
| title_short | The Integrity of the HMR complex is necessary for centromeric binding and reproductive isolation in Drosophila. |
| title_sort | integrity of the hmr complex is necessary for centromeric binding and reproductive isolation in drosophila |
| url | https://doi.org/10.1371/journal.pgen.1009744 |
| work_keys_str_mv | AT andrealukacs theintegrityofthehmrcomplexisnecessaryforcentromericbindingandreproductiveisolationindrosophila AT andreaswthomae theintegrityofthehmrcomplexisnecessaryforcentromericbindingandreproductiveisolationindrosophila AT peterkrueger theintegrityofthehmrcomplexisnecessaryforcentromericbindingandreproductiveisolationindrosophila AT tamasschauer theintegrityofthehmrcomplexisnecessaryforcentromericbindingandreproductiveisolationindrosophila AT anuroopvvenkatasubramani theintegrityofthehmrcomplexisnecessaryforcentromericbindingandreproductiveisolationindrosophila AT nataliaykochanova theintegrityofthehmrcomplexisnecessaryforcentromericbindingandreproductiveisolationindrosophila AT wasimaftab theintegrityofthehmrcomplexisnecessaryforcentromericbindingandreproductiveisolationindrosophila AT rupamchoudhury theintegrityofthehmrcomplexisnecessaryforcentromericbindingandreproductiveisolationindrosophila AT ignasiforne theintegrityofthehmrcomplexisnecessaryforcentromericbindingandreproductiveisolationindrosophila AT axelimhof theintegrityofthehmrcomplexisnecessaryforcentromericbindingandreproductiveisolationindrosophila AT andrealukacs integrityofthehmrcomplexisnecessaryforcentromericbindingandreproductiveisolationindrosophila AT andreaswthomae integrityofthehmrcomplexisnecessaryforcentromericbindingandreproductiveisolationindrosophila AT peterkrueger integrityofthehmrcomplexisnecessaryforcentromericbindingandreproductiveisolationindrosophila AT tamasschauer integrityofthehmrcomplexisnecessaryforcentromericbindingandreproductiveisolationindrosophila AT anuroopvvenkatasubramani integrityofthehmrcomplexisnecessaryforcentromericbindingandreproductiveisolationindrosophila AT nataliaykochanova integrityofthehmrcomplexisnecessaryforcentromericbindingandreproductiveisolationindrosophila AT wasimaftab integrityofthehmrcomplexisnecessaryforcentromericbindingandreproductiveisolationindrosophila AT rupamchoudhury integrityofthehmrcomplexisnecessaryforcentromericbindingandreproductiveisolationindrosophila AT ignasiforne integrityofthehmrcomplexisnecessaryforcentromericbindingandreproductiveisolationindrosophila AT axelimhof integrityofthehmrcomplexisnecessaryforcentromericbindingandreproductiveisolationindrosophila |