Ex Vivo Determination of Broadband Absorption and Effective Scattering Coefficients of Porcine Tissue

A novel approach for precise determination of the optical scattering and absorption properties of porcine tissue using an optimized integrating sphere setup was applied. Measurements on several sample types (skin, muscle, adipose tissue, bone, cartilage, brain) in the spectral range between 400 nm a...

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Bibliographic Details
Main Authors: Florian Bergmann, Florian Foschum, Leonie Marzel, Alwin Kienle
Format: Article
Language:English
Published: MDPI AG 2021-08-01
Series:Photonics
Subjects:
Online Access:https://www.mdpi.com/2304-6732/8/9/365
Description
Summary:A novel approach for precise determination of the optical scattering and absorption properties of porcine tissue using an optimized integrating sphere setup was applied. Measurements on several sample types (skin, muscle, adipose tissue, bone, cartilage, brain) in the spectral range between 400 nm and 1400 nm were performed. Due to the heterogeneity of biological samples, measurements on different individual animals as well as on different sections for each sample type were carried out. For all samples, we used an index matching method to reduce surface roughness effects and to prevent dehydration. The derived absorption spectra were used to estimate the concentration of important tissue chromophores such as water, oxy- and deoxyhemoglobin, collagen and fat.
ISSN:2304-6732