Study on the Effect of Ziziphi spinosae Semen Poria cocos Powder and Soybean Peptide Powder on Improving Sleep in Insomnia Model Mice
Objective: To study the sleep-improving effect of Ziziphi spinosae Semen Poria cocos powder and soybean peptide powder on insomnia model mice induced by p-chlorophenylalanine (PCPA). Methods: One hundred eighty SPF male BALB/c mice were purchased. First, ninety of them were randomly divided into six...
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2023-04-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022050374 |
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| author | Rong WEI Zhen WANG Huaiping LI Yu GUO Jian ZHAO |
| author_facet | Rong WEI Zhen WANG Huaiping LI Yu GUO Jian ZHAO |
| author_sort | Rong WEI |
| collection | DOAJ |
| description | Objective: To study the sleep-improving effect of Ziziphi spinosae Semen Poria cocos powder and soybean peptide powder on insomnia model mice induced by p-chlorophenylalanine (PCPA). Methods: One hundred eighty SPF male BALB/c mice were purchased. First, ninety of them were randomly divided into six groups (normal control group, model control group, low, medium and high dose groups of Ziziphi spinosae Semen Poria cocos powder, soybean peptide powder group) for the pentobarbital sodium-induced sleep test. The remaining 90 mice were randomly divided into six groups (same group as above) for the open field test, the contents of hypothalamic 5-hydroxytryptamine (5-HT), 5-hydroxyindoleacetic acid (5-HIAA) and norepinephrine (NE) in the hypothalamus were measured and the contents of γ-aminobutyric acid (GABA) and glutamic acid (Glu) in the hippocampus were measured. The normal control group and the model control group were administered with sterile water while the low, medium and high dose groups of Ziziphi spinosae Semen Poria cocos powder were administered with 0.67, 1.33, 4.00 g/kg BW solution and the soybean peptide powder groupwas administered with 1.15 g/kg BW solution. The gavage volume of rats was 20 mL/kg BW. The sleep test lasted for 36 days and the open field test lasted for 37 days. On the 29th and 30th day, except the normal control group, the other mice were given intraperitoneal injection of PCPA (350 mg/kg BW) to construct an insomnia model and then continued to be administered for 6, 7 d. Results: Compared with the model control group, Ziziphi spinosae Semen Poria cocos powder could significantly shorten the sleep latency induced by pentobarbital sodium, prolong sleep time, reduce horizontal score, vertical score and modified score, significantly increase the contents of 5-HT and 5-HIAA in the hypothalamus, reducing NE content, reduce Glu content in the hippocampus, improve the hippocampal GABA content, regulate the Glu/GABA ratio and maintain Glu/GABA balance in the hippocampus. Soybean peptide powder could significantly prolong sleep time and maintain Glu/GABA balance. Conclusion: Ziziphi spinosae Semen Poria cocos powder has a certain ameliorative effect on sleep in insomnia model mice by elevating monoamine neurotransmitter 5-HT content and reducing NE content in the hypothalamic of insomnia mice, promoting the sleep wake state of insomnia mice toward normal recovery. It also can regulate Glu and GABA content in the hippocampal and maintain the homeostatic balance of Glu and GABA. |
| first_indexed | 2024-04-09T22:16:18Z |
| format | Article |
| id | doaj.art-8c8c691f0e71407b8016fac60b0d39e4 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-09T22:16:18Z |
| publishDate | 2023-04-01 |
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| series | Shipin gongye ke-ji |
| spelling | doaj.art-8c8c691f0e71407b8016fac60b0d39e42023-03-23T01:41:47ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062023-04-0144735936610.13386/j.issn1002-0306.20220503742022050374-7Study on the Effect of Ziziphi spinosae Semen Poria cocos Powder and Soybean Peptide Powder on Improving Sleep in Insomnia Model MiceRong WEI0Zhen WANG1Huaiping LI2Yu GUO3Jian ZHAO4Testing Center of Health Food Function Determination, College of Arts and Science, Beijing Union University, Beijing 100191, ChinaArura Tibetan Medicine (Shandong) Health Industry Co., Ltd., Jinan 250104, ChinaArura Tibetan Medicine (Shandong) Health Industry Co., Ltd., Jinan 250104, ChinaBeijing Key Laboratory of Bioactive Substances and Functional Food, Beijing Union University, Beijing 100191, ChinaBeijing Key Laboratory of Bioactive Substances and Functional Food, Beijing Union University, Beijing 100191, ChinaObjective: To study the sleep-improving effect of Ziziphi spinosae Semen Poria cocos powder and soybean peptide powder on insomnia model mice induced by p-chlorophenylalanine (PCPA). Methods: One hundred eighty SPF male BALB/c mice were purchased. First, ninety of them were randomly divided into six groups (normal control group, model control group, low, medium and high dose groups of Ziziphi spinosae Semen Poria cocos powder, soybean peptide powder group) for the pentobarbital sodium-induced sleep test. The remaining 90 mice were randomly divided into six groups (same group as above) for the open field test, the contents of hypothalamic 5-hydroxytryptamine (5-HT), 5-hydroxyindoleacetic acid (5-HIAA) and norepinephrine (NE) in the hypothalamus were measured and the contents of γ-aminobutyric acid (GABA) and glutamic acid (Glu) in the hippocampus were measured. The normal control group and the model control group were administered with sterile water while the low, medium and high dose groups of Ziziphi spinosae Semen Poria cocos powder were administered with 0.67, 1.33, 4.00 g/kg BW solution and the soybean peptide powder groupwas administered with 1.15 g/kg BW solution. The gavage volume of rats was 20 mL/kg BW. The sleep test lasted for 36 days and the open field test lasted for 37 days. On the 29th and 30th day, except the normal control group, the other mice were given intraperitoneal injection of PCPA (350 mg/kg BW) to construct an insomnia model and then continued to be administered for 6, 7 d. Results: Compared with the model control group, Ziziphi spinosae Semen Poria cocos powder could significantly shorten the sleep latency induced by pentobarbital sodium, prolong sleep time, reduce horizontal score, vertical score and modified score, significantly increase the contents of 5-HT and 5-HIAA in the hypothalamus, reducing NE content, reduce Glu content in the hippocampus, improve the hippocampal GABA content, regulate the Glu/GABA ratio and maintain Glu/GABA balance in the hippocampus. Soybean peptide powder could significantly prolong sleep time and maintain Glu/GABA balance. Conclusion: Ziziphi spinosae Semen Poria cocos powder has a certain ameliorative effect on sleep in insomnia model mice by elevating monoamine neurotransmitter 5-HT content and reducing NE content in the hypothalamic of insomnia mice, promoting the sleep wake state of insomnia mice toward normal recovery. It also can regulate Glu and GABA content in the hippocampal and maintain the homeostatic balance of Glu and GABA.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022050374ziziphi spinosae semenporia cocossoybean peptidesleep improvement5-htgaba |
| spellingShingle | Rong WEI Zhen WANG Huaiping LI Yu GUO Jian ZHAO Study on the Effect of Ziziphi spinosae Semen Poria cocos Powder and Soybean Peptide Powder on Improving Sleep in Insomnia Model Mice Shipin gongye ke-ji ziziphi spinosae semen poria cocos soybean peptide sleep improvement 5-ht gaba |
| title | Study on the Effect of Ziziphi spinosae Semen Poria cocos Powder and Soybean Peptide Powder on Improving Sleep in Insomnia Model Mice |
| title_full | Study on the Effect of Ziziphi spinosae Semen Poria cocos Powder and Soybean Peptide Powder on Improving Sleep in Insomnia Model Mice |
| title_fullStr | Study on the Effect of Ziziphi spinosae Semen Poria cocos Powder and Soybean Peptide Powder on Improving Sleep in Insomnia Model Mice |
| title_full_unstemmed | Study on the Effect of Ziziphi spinosae Semen Poria cocos Powder and Soybean Peptide Powder on Improving Sleep in Insomnia Model Mice |
| title_short | Study on the Effect of Ziziphi spinosae Semen Poria cocos Powder and Soybean Peptide Powder on Improving Sleep in Insomnia Model Mice |
| title_sort | study on the effect of ziziphi spinosae semen poria cocos powder and soybean peptide powder on improving sleep in insomnia model mice |
| topic | ziziphi spinosae semen poria cocos soybean peptide sleep improvement 5-ht gaba |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022050374 |
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