An Exploration of Seaweed Polysaccharides Stimulating Denitrifying Bacteria for Safer Nitrate Removal
Excessive use of nitrogen fertilizer in intensively managed agriculture has resulted in abundant accumulation of nitrate in soil, which limits agriculture sustainability. How to reduce nitrate content is the key to alleviate secondary soil salinization. However, the microorganisms used in soil remed...
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MDPI AG
2021-06-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/11/3390 |
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| author | Hui Zhang Lin Song Xiaolin Chen Pengcheng Li |
| author_facet | Hui Zhang Lin Song Xiaolin Chen Pengcheng Li |
| author_sort | Hui Zhang |
| collection | DOAJ |
| description | Excessive use of nitrogen fertilizer in intensively managed agriculture has resulted in abundant accumulation of nitrate in soil, which limits agriculture sustainability. How to reduce nitrate content is the key to alleviate secondary soil salinization. However, the microorganisms used in soil remediation cause some problems such as weak efficiency and short survival time. In this study, seaweed polysaccharides were used as stimulant to promote the rapid growth and safer nitrate removal of denitrifying bacteria. Firstly, the growth rate and NO<sub>3</sub><sup>−</sup>-N removal capacity of three kinds of denitrifying bacteria, <i>Bacillus subtilis</i> (BS), <i>Pseudomonas stutzeri</i> (PS) and <i>Pseudomonas putida</i> (PP), were compared. The results showed that <i>Bacillus subtilis</i> (BS) had a faster growth rate and stronger nitrate removal ability. We then studied the effects of <i>Enteromorpha linza</i> polysaccharides (EP), carrageenan (CA), and sodium alginate (AL) on growth and denitrification performance of <i>Bacillus subtilis</i> (BS). The results showed that seaweed polysaccharides obviously promoted the growth of <i>Bacillus subtilis</i> (BS), and accelerated the reduction of NO<sub>3</sub><sup>−</sup>-N. More importantly, the increased NH<sub>4</sub><sup>+</sup>-N content could avoid excessive loss of nitrogen, and less NO<sub>2</sub><sup>−</sup>-N accumulation could avoid toxic effects on plants. This new strategy of using denitrifying bacteria for safely remediating secondary soil salinization has a great significance. |
| first_indexed | 2024-03-10T10:44:04Z |
| format | Article |
| id | doaj.art-8c90ffdbb8fa48be97d554931feaaa03 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T10:44:04Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-8c90ffdbb8fa48be97d554931feaaa032023-11-21T22:43:59ZengMDPI AGMolecules1420-30492021-06-012611339010.3390/molecules26113390An Exploration of Seaweed Polysaccharides Stimulating Denitrifying Bacteria for Safer Nitrate RemovalHui Zhang0Lin Song1Xiaolin Chen2Pengcheng Li3College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao 266042, ChinaCollege of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao 266042, ChinaCAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaCAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaExcessive use of nitrogen fertilizer in intensively managed agriculture has resulted in abundant accumulation of nitrate in soil, which limits agriculture sustainability. How to reduce nitrate content is the key to alleviate secondary soil salinization. However, the microorganisms used in soil remediation cause some problems such as weak efficiency and short survival time. In this study, seaweed polysaccharides were used as stimulant to promote the rapid growth and safer nitrate removal of denitrifying bacteria. Firstly, the growth rate and NO<sub>3</sub><sup>−</sup>-N removal capacity of three kinds of denitrifying bacteria, <i>Bacillus subtilis</i> (BS), <i>Pseudomonas stutzeri</i> (PS) and <i>Pseudomonas putida</i> (PP), were compared. The results showed that <i>Bacillus subtilis</i> (BS) had a faster growth rate and stronger nitrate removal ability. We then studied the effects of <i>Enteromorpha linza</i> polysaccharides (EP), carrageenan (CA), and sodium alginate (AL) on growth and denitrification performance of <i>Bacillus subtilis</i> (BS). The results showed that seaweed polysaccharides obviously promoted the growth of <i>Bacillus subtilis</i> (BS), and accelerated the reduction of NO<sub>3</sub><sup>−</sup>-N. More importantly, the increased NH<sub>4</sub><sup>+</sup>-N content could avoid excessive loss of nitrogen, and less NO<sub>2</sub><sup>−</sup>-N accumulation could avoid toxic effects on plants. This new strategy of using denitrifying bacteria for safely remediating secondary soil salinization has a great significance.https://www.mdpi.com/1420-3049/26/11/3390seaweed polysaccharidesdenitrifying bacterianitrate removalgrowth promotion |
| spellingShingle | Hui Zhang Lin Song Xiaolin Chen Pengcheng Li An Exploration of Seaweed Polysaccharides Stimulating Denitrifying Bacteria for Safer Nitrate Removal Molecules seaweed polysaccharides denitrifying bacteria nitrate removal growth promotion |
| title | An Exploration of Seaweed Polysaccharides Stimulating Denitrifying Bacteria for Safer Nitrate Removal |
| title_full | An Exploration of Seaweed Polysaccharides Stimulating Denitrifying Bacteria for Safer Nitrate Removal |
| title_fullStr | An Exploration of Seaweed Polysaccharides Stimulating Denitrifying Bacteria for Safer Nitrate Removal |
| title_full_unstemmed | An Exploration of Seaweed Polysaccharides Stimulating Denitrifying Bacteria for Safer Nitrate Removal |
| title_short | An Exploration of Seaweed Polysaccharides Stimulating Denitrifying Bacteria for Safer Nitrate Removal |
| title_sort | exploration of seaweed polysaccharides stimulating denitrifying bacteria for safer nitrate removal |
| topic | seaweed polysaccharides denitrifying bacteria nitrate removal growth promotion |
| url | https://www.mdpi.com/1420-3049/26/11/3390 |
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