Anthocyanins from <i>Hibiscus syriacus</i> L. Inhibit Oxidative Stress-Mediated Apoptosis by Activating the Nrf2/HO-1 Signaling Pathway
<i>Hibiscus syriacus</i> L. is distributed widely throughout Eastern and Southern Asia and considered as the national flower of South Korea. The extraction of several plant parts of <i>H. syriacus</i> L. is currently used as a natural remedy for several diseases, including br...
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MDPI AG
2020-01-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/9/1/42 |
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| author | Ilandarage Menu Neelaka Molagoda Kyoung Tae Lee Yung Hyun Choi Gi-Young Kim |
| author_facet | Ilandarage Menu Neelaka Molagoda Kyoung Tae Lee Yung Hyun Choi Gi-Young Kim |
| author_sort | Ilandarage Menu Neelaka Molagoda |
| collection | DOAJ |
| description | <i>Hibiscus syriacus</i> L. is distributed widely throughout Eastern and Southern Asia and considered as the national flower of South Korea. The extraction of several plant parts of <i>H. syriacus</i> L. is currently used as a natural remedy for several diseases, including breast and lung cancer, microbial infection, and chronic inflammation. However, the effect of the anthocyanin extract of <i>H. syriacus</i> L. petals (PS) in oxidative stress conditions has not been studied. In this study, we evaluated the cytoprotective effect of PS against H<sub>2</sub>O<sub>2</sub>-induced oxidative stress in HaCaT keratinocytes. In this study, we found that PS significantly inhibited H<sub>2</sub>O<sub>2</sub>-induced apoptosis of HaCaT keratinocytes. We also revealed that PS mediated-cytoprotective effect was associated with the increased expression of heme oxygenase-1 (HO-1) arising from the activation of nuclear factor erythroid 2-related factor-2 (Nrf2). PS also decreased H<sub>2</sub>O<sub>2</sub>-induced excessive intracellular ROS generation and restored H<sub>2</sub>O<sub>2</sub>-induced mitochondrial depolarization through the downregulation of mitochondrial ROS production. Furthermore, H<sub>2</sub>O<sub>2</sub>-induced Bax and caspase-3 expression was markedly abolished in the presence of PS. The inhibition of HO-1 by zinc protoporphyrin significantly attenuated the cytoprotective effect of PS in H<sub>2</sub>O<sub>2</sub>-treated HaCaT keratinocytes along with ROS generation, indicating that HO-1 crucially affects PS-mediated cytoprotective properties. Collectively, our results suggested that, under H<sub>2</sub>O<sub>2</sub>-mediated oxidative stress conditions, PS sustained a normal level of mitochondrial membrane potential and ROS generation in HaCaT keratinocytes by activating the Nrf2/HO-1 axis, exerting cytoprotective effects against oxidative stress. |
| first_indexed | 2024-03-12T10:39:50Z |
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| id | doaj.art-8c933abe8c574769b2beb5f4234a2ef4 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-12T10:39:50Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
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| series | Antioxidants |
| spelling | doaj.art-8c933abe8c574769b2beb5f4234a2ef42023-09-02T08:18:50ZengMDPI AGAntioxidants2076-39212020-01-01914210.3390/antiox9010042antiox9010042Anthocyanins from <i>Hibiscus syriacus</i> L. Inhibit Oxidative Stress-Mediated Apoptosis by Activating the Nrf2/HO-1 Signaling PathwayIlandarage Menu Neelaka Molagoda0Kyoung Tae Lee1Yung Hyun Choi2Gi-Young Kim3Department of Marine Life Sciences, Jeju National University, Jeju 63243, KoreaForest Biomaterials Research Center, National Institute of Forest Science, Jinju 52817, KoreaDepartment of Biochemistry, College of Oriental Medicine, Dong-Eui University, Busan 47227, KoreaDepartment of Marine Life Sciences, Jeju National University, Jeju 63243, Korea<i>Hibiscus syriacus</i> L. is distributed widely throughout Eastern and Southern Asia and considered as the national flower of South Korea. The extraction of several plant parts of <i>H. syriacus</i> L. is currently used as a natural remedy for several diseases, including breast and lung cancer, microbial infection, and chronic inflammation. However, the effect of the anthocyanin extract of <i>H. syriacus</i> L. petals (PS) in oxidative stress conditions has not been studied. In this study, we evaluated the cytoprotective effect of PS against H<sub>2</sub>O<sub>2</sub>-induced oxidative stress in HaCaT keratinocytes. In this study, we found that PS significantly inhibited H<sub>2</sub>O<sub>2</sub>-induced apoptosis of HaCaT keratinocytes. We also revealed that PS mediated-cytoprotective effect was associated with the increased expression of heme oxygenase-1 (HO-1) arising from the activation of nuclear factor erythroid 2-related factor-2 (Nrf2). PS also decreased H<sub>2</sub>O<sub>2</sub>-induced excessive intracellular ROS generation and restored H<sub>2</sub>O<sub>2</sub>-induced mitochondrial depolarization through the downregulation of mitochondrial ROS production. Furthermore, H<sub>2</sub>O<sub>2</sub>-induced Bax and caspase-3 expression was markedly abolished in the presence of PS. The inhibition of HO-1 by zinc protoporphyrin significantly attenuated the cytoprotective effect of PS in H<sub>2</sub>O<sub>2</sub>-treated HaCaT keratinocytes along with ROS generation, indicating that HO-1 crucially affects PS-mediated cytoprotective properties. Collectively, our results suggested that, under H<sub>2</sub>O<sub>2</sub>-mediated oxidative stress conditions, PS sustained a normal level of mitochondrial membrane potential and ROS generation in HaCaT keratinocytes by activating the Nrf2/HO-1 axis, exerting cytoprotective effects against oxidative stress.https://www.mdpi.com/2076-3921/9/1/42<i>hibiscus syriacus</i> l.oxidative stressreactive oxygen speciesnuclear factor erythroid 2-related factor-2heme oxygenase-1 |
| spellingShingle | Ilandarage Menu Neelaka Molagoda Kyoung Tae Lee Yung Hyun Choi Gi-Young Kim Anthocyanins from <i>Hibiscus syriacus</i> L. Inhibit Oxidative Stress-Mediated Apoptosis by Activating the Nrf2/HO-1 Signaling Pathway Antioxidants <i>hibiscus syriacus</i> l. oxidative stress reactive oxygen species nuclear factor erythroid 2-related factor-2 heme oxygenase-1 |
| title | Anthocyanins from <i>Hibiscus syriacus</i> L. Inhibit Oxidative Stress-Mediated Apoptosis by Activating the Nrf2/HO-1 Signaling Pathway |
| title_full | Anthocyanins from <i>Hibiscus syriacus</i> L. Inhibit Oxidative Stress-Mediated Apoptosis by Activating the Nrf2/HO-1 Signaling Pathway |
| title_fullStr | Anthocyanins from <i>Hibiscus syriacus</i> L. Inhibit Oxidative Stress-Mediated Apoptosis by Activating the Nrf2/HO-1 Signaling Pathway |
| title_full_unstemmed | Anthocyanins from <i>Hibiscus syriacus</i> L. Inhibit Oxidative Stress-Mediated Apoptosis by Activating the Nrf2/HO-1 Signaling Pathway |
| title_short | Anthocyanins from <i>Hibiscus syriacus</i> L. Inhibit Oxidative Stress-Mediated Apoptosis by Activating the Nrf2/HO-1 Signaling Pathway |
| title_sort | anthocyanins from i hibiscus syriacus i l inhibit oxidative stress mediated apoptosis by activating the nrf2 ho 1 signaling pathway |
| topic | <i>hibiscus syriacus</i> l. oxidative stress reactive oxygen species nuclear factor erythroid 2-related factor-2 heme oxygenase-1 |
| url | https://www.mdpi.com/2076-3921/9/1/42 |
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