Spatial organization of enzymes to enhance synthetic pathways in microbial chassis: a systematic review
Abstract For years, microbes have been widely applied as chassis in the construction of synthetic metabolic pathways. However, the lack of in vivo enzyme clustering of heterologous metabolic pathways in these organisms often results in low local concentrations of enzymes and substrates, leading to a...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-07-01
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| Series: | Microbial Cell Factories |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12934-018-0965-0 |
| _version_ | 1828517591679762432 |
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| author | Xin-yuan Qiu Si-Si Xie Lu Min Xiao-Min Wu Lv-Yun Zhu Lingyun Zhu |
| author_facet | Xin-yuan Qiu Si-Si Xie Lu Min Xiao-Min Wu Lv-Yun Zhu Lingyun Zhu |
| author_sort | Xin-yuan Qiu |
| collection | DOAJ |
| description | Abstract For years, microbes have been widely applied as chassis in the construction of synthetic metabolic pathways. However, the lack of in vivo enzyme clustering of heterologous metabolic pathways in these organisms often results in low local concentrations of enzymes and substrates, leading to a low productive efficacy. In recent years, multiple methods have been applied to the construction of small metabolic clusters by spatial organization of heterologous metabolic enzymes. These methods mainly focused on using engineered molecules to bring the enzymes into close proximity via different interaction mechanisms among proteins and nucleotides and have been applied in various heterologous pathways with different degrees of success while facing numerous challenges. In this paper, we mainly reviewed some of those notable advances in designing and creating approaches to achieve spatial organization using different intermolecular interactions. Current challenges and future aspects in the further application of such approaches are also discussed in this paper. |
| first_indexed | 2024-12-11T18:43:31Z |
| format | Article |
| id | doaj.art-8c93dba6a1b148a584a95f81488f67fe |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-12-11T18:43:31Z |
| publishDate | 2018-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-8c93dba6a1b148a584a95f81488f67fe2022-12-22T00:54:32ZengBMCMicrobial Cell Factories1475-28592018-07-011711710.1186/s12934-018-0965-0Spatial organization of enzymes to enhance synthetic pathways in microbial chassis: a systematic reviewXin-yuan Qiu0Si-Si Xie1Lu Min2Xiao-Min Wu3Lv-Yun Zhu4Lingyun Zhu5Department of Biology and Chemistry, College of Liberal Arts and Sciences, National University of Defense TechnologyDepartment of Biology and Chemistry, College of Liberal Arts and Sciences, National University of Defense TechnologyDepartment of Biology and Chemistry, College of Liberal Arts and Sciences, National University of Defense TechnologyDepartment of Biology and Chemistry, College of Liberal Arts and Sciences, National University of Defense TechnologyDepartment of Biology and Chemistry, College of Liberal Arts and Sciences, National University of Defense TechnologyDepartment of Biology and Chemistry, College of Liberal Arts and Sciences, National University of Defense TechnologyAbstract For years, microbes have been widely applied as chassis in the construction of synthetic metabolic pathways. However, the lack of in vivo enzyme clustering of heterologous metabolic pathways in these organisms often results in low local concentrations of enzymes and substrates, leading to a low productive efficacy. In recent years, multiple methods have been applied to the construction of small metabolic clusters by spatial organization of heterologous metabolic enzymes. These methods mainly focused on using engineered molecules to bring the enzymes into close proximity via different interaction mechanisms among proteins and nucleotides and have been applied in various heterologous pathways with different degrees of success while facing numerous challenges. In this paper, we mainly reviewed some of those notable advances in designing and creating approaches to achieve spatial organization using different intermolecular interactions. Current challenges and future aspects in the further application of such approaches are also discussed in this paper.http://link.springer.com/article/10.1186/s12934-018-0965-0Spatial organizationEnzyme clusteringPathway enhancementMolecular scaffold |
| spellingShingle | Xin-yuan Qiu Si-Si Xie Lu Min Xiao-Min Wu Lv-Yun Zhu Lingyun Zhu Spatial organization of enzymes to enhance synthetic pathways in microbial chassis: a systematic review Microbial Cell Factories Spatial organization Enzyme clustering Pathway enhancement Molecular scaffold |
| title | Spatial organization of enzymes to enhance synthetic pathways in microbial chassis: a systematic review |
| title_full | Spatial organization of enzymes to enhance synthetic pathways in microbial chassis: a systematic review |
| title_fullStr | Spatial organization of enzymes to enhance synthetic pathways in microbial chassis: a systematic review |
| title_full_unstemmed | Spatial organization of enzymes to enhance synthetic pathways in microbial chassis: a systematic review |
| title_short | Spatial organization of enzymes to enhance synthetic pathways in microbial chassis: a systematic review |
| title_sort | spatial organization of enzymes to enhance synthetic pathways in microbial chassis a systematic review |
| topic | Spatial organization Enzyme clustering Pathway enhancement Molecular scaffold |
| url | http://link.springer.com/article/10.1186/s12934-018-0965-0 |
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