Single cell analysis of transcriptome and open chromatin reveals the dynamics of hair follicle stem cell aging
Aging is defined as the functional decline of tissues and organisms, leading to many human conditions, such as cancer, neurodegenerative diseases, and hair loss. Although stem cell exhaustion is widely recognized as a hallmark of aging, our understanding of cell state changes–specifically, the dynam...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-07-01
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| Series: | Frontiers in Aging |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fragi.2023.1192149/full |
| _version_ | 1797789226645848064 |
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| author | Chi Zhang Dongmei Wang Dongmei Wang Dongmei Wang Robin Dowell Rui Yi Rui Yi Rui Yi |
| author_facet | Chi Zhang Dongmei Wang Dongmei Wang Dongmei Wang Robin Dowell Rui Yi Rui Yi Rui Yi |
| author_sort | Chi Zhang |
| collection | DOAJ |
| description | Aging is defined as the functional decline of tissues and organisms, leading to many human conditions, such as cancer, neurodegenerative diseases, and hair loss. Although stem cell exhaustion is widely recognized as a hallmark of aging, our understanding of cell state changes–specifically, the dynamics of the transcriptome and open chromatin landscape, and their relationship with aging–remains incomplete. Here we present a longitudinal, single-cell atlas of the transcriptome and open chromatin landscape for epithelia cells of the skin across various hair cycle stages and ages in mice. Our findings reveal fluctuating hair follicle stem cell (HF-SC) states, some of which are associated with the progression of the hair cycle during aging. Conversely, inner bulge niche cells display a more linear progression, seemingly less affected by the hair cycle. Further analysis of the open chromatin landscape, determined by single-cell Assay for Transposase-Accessible Chromatin (ATAC) sequencing, demonstrates that reduced open chromatin regions in HF-SCs are associated with differentiation, whereas gained open chromatin regions in HF-SCs are linked to the transcriptional control of quiescence. These findings enhance our understanding of the transcriptional dynamics in HF-SC aging and lay the molecular groundwork for investigating and potentially reversing the aging process in future experimental studies. |
| first_indexed | 2024-03-13T01:47:42Z |
| format | Article |
| id | doaj.art-8c963f1371584994b519ba1cce749750 |
| institution | Directory Open Access Journal |
| issn | 2673-6217 |
| language | English |
| last_indexed | 2024-03-13T01:47:42Z |
| publishDate | 2023-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Aging |
| spelling | doaj.art-8c963f1371584994b519ba1cce7497502023-07-03T06:35:59ZengFrontiers Media S.A.Frontiers in Aging2673-62172023-07-01410.3389/fragi.2023.11921491192149Single cell analysis of transcriptome and open chromatin reveals the dynamics of hair follicle stem cell agingChi Zhang0Dongmei Wang1Dongmei Wang2Dongmei Wang3Robin Dowell4Rui Yi5Rui Yi6Rui Yi7Department of Pathology, Northwestern University Feinberg School of Medicine, Chicago, IL, United StatesDepartment of Pathology, Northwestern University Feinberg School of Medicine, Chicago, IL, United StatesDepartment of Dermatology, Northwestern University Feinberg School of Medicine, Chicago, IL, United StatesRobert H. Lurie Comprehensive Cancer Center, Northwestern University Feinberg School of Medicine, Chicago, IL, United StatesBioFrontiers Institute, University of Colorado Boulder, Boulder, CO, United StatesDepartment of Pathology, Northwestern University Feinberg School of Medicine, Chicago, IL, United StatesDepartment of Dermatology, Northwestern University Feinberg School of Medicine, Chicago, IL, United StatesRobert H. Lurie Comprehensive Cancer Center, Northwestern University Feinberg School of Medicine, Chicago, IL, United StatesAging is defined as the functional decline of tissues and organisms, leading to many human conditions, such as cancer, neurodegenerative diseases, and hair loss. Although stem cell exhaustion is widely recognized as a hallmark of aging, our understanding of cell state changes–specifically, the dynamics of the transcriptome and open chromatin landscape, and their relationship with aging–remains incomplete. Here we present a longitudinal, single-cell atlas of the transcriptome and open chromatin landscape for epithelia cells of the skin across various hair cycle stages and ages in mice. Our findings reveal fluctuating hair follicle stem cell (HF-SC) states, some of which are associated with the progression of the hair cycle during aging. Conversely, inner bulge niche cells display a more linear progression, seemingly less affected by the hair cycle. Further analysis of the open chromatin landscape, determined by single-cell Assay for Transposase-Accessible Chromatin (ATAC) sequencing, demonstrates that reduced open chromatin regions in HF-SCs are associated with differentiation, whereas gained open chromatin regions in HF-SCs are linked to the transcriptional control of quiescence. These findings enhance our understanding of the transcriptional dynamics in HF-SC aging and lay the molecular groundwork for investigating and potentially reversing the aging process in future experimental studies.https://www.frontiersin.org/articles/10.3389/fragi.2023.1192149/fullhair follicle stem cellstem cell exhaustionquiescencecell adhesionsingle cell genomicsscRNAseq |
| spellingShingle | Chi Zhang Dongmei Wang Dongmei Wang Dongmei Wang Robin Dowell Rui Yi Rui Yi Rui Yi Single cell analysis of transcriptome and open chromatin reveals the dynamics of hair follicle stem cell aging Frontiers in Aging hair follicle stem cell stem cell exhaustion quiescence cell adhesion single cell genomics scRNAseq |
| title | Single cell analysis of transcriptome and open chromatin reveals the dynamics of hair follicle stem cell aging |
| title_full | Single cell analysis of transcriptome and open chromatin reveals the dynamics of hair follicle stem cell aging |
| title_fullStr | Single cell analysis of transcriptome and open chromatin reveals the dynamics of hair follicle stem cell aging |
| title_full_unstemmed | Single cell analysis of transcriptome and open chromatin reveals the dynamics of hair follicle stem cell aging |
| title_short | Single cell analysis of transcriptome and open chromatin reveals the dynamics of hair follicle stem cell aging |
| title_sort | single cell analysis of transcriptome and open chromatin reveals the dynamics of hair follicle stem cell aging |
| topic | hair follicle stem cell stem cell exhaustion quiescence cell adhesion single cell genomics scRNAseq |
| url | https://www.frontiersin.org/articles/10.3389/fragi.2023.1192149/full |
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