Can Magnetic Targeting of Magnetically Labeled Circulating Cells Optimize Intramyocardial Cell Retention?
Therapeutic intracavitary stem cell infusion currently suffers from poor myocardial homing. We examined whether cardiac cell retention could be enhanced by magnetic targeting of endothelial progenitor cells (EPCs) loaded with iron oxide nanoparticles. EPCs were magnetically labeled with citrate-coat...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2012-04-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.3727/096368911X612440 |
| _version_ | 1819019611549466624 |
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| author | Aurélie Chaudeurge Claire Wilhelm Annabel Chen-Tournoux Patrick Farahmand Valérie Bellamy Gwennhael Autret Christine Ménager Albert Hagège Jerome Larghéro Florence Gazeau Olivier Clément Philippe Menasché |
| author_facet | Aurélie Chaudeurge Claire Wilhelm Annabel Chen-Tournoux Patrick Farahmand Valérie Bellamy Gwennhael Autret Christine Ménager Albert Hagège Jerome Larghéro Florence Gazeau Olivier Clément Philippe Menasché |
| author_sort | Aurélie Chaudeurge |
| collection | DOAJ |
| description | Therapeutic intracavitary stem cell infusion currently suffers from poor myocardial homing. We examined whether cardiac cell retention could be enhanced by magnetic targeting of endothelial progenitor cells (EPCs) loaded with iron oxide nanoparticles. EPCs were magnetically labeled with citrate-coated iron oxide nanoparticles. Cell proliferation, migration, and CXCR4 chemokine receptor expression were assessed in different labeling conditions and no adverse effects of the magnetic label were observed. The magnetophoretic mobility of labeled EPCs was determined in vitro, with the same magnet as that subsequently used in vivo. Coronary artery occlusion was induced for 30 min in 36 rats (31 survivors), followed by 20 min of reperfusion. The rats were randomized to receive, during brief aortic cross-clamping, direct intraventricular injection of culture medium ( n = 7) or magnetically labeled EPCs ( n = 24), with ( n = 14) or without ( n = 10) subcutaneous insertion of a magnet over the chest cavity ( n = 14). The hearts were explanted 24 h later and engrafted cells were visualized by magnetic resonance imaging (MRI) of the heart at 1.5 T. Their abundance in the myocardium was also analyzed semiquantitatively by immunofluorescence, and quantitatively by real-time polymerase chain reaction (RT-PCR). Although differences in cell retention between groups failed to be statistically significant using RT-PCR quantification, due to the variability of the animal model, immunostaining showed that the average number of engrafted EPCs was significantly ten times higher with than without magnetic targeting. There was thus a consistent trend favoring the magnet-treated hearts, thereby suggesting magnetic targeting as a potentially new mean of enhancing myocardial homing of intravascularly delivered stem cells. Magnetic targeting has the potential to enhance myocardial retention of intravascularly delivered endothelial progenitor cells. |
| first_indexed | 2024-12-21T03:38:04Z |
| format | Article |
| id | doaj.art-8c9d9c65620c47ffb735e26a939e3022 |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-21T03:38:04Z |
| publishDate | 2012-04-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-8c9d9c65620c47ffb735e26a939e30222022-12-21T19:17:17ZengSAGE PublishingCell Transplantation0963-68971555-38922012-04-012110.3727/096368911X612440Can Magnetic Targeting of Magnetically Labeled Circulating Cells Optimize Intramyocardial Cell Retention?Aurélie Chaudeurge0Claire Wilhelm1Annabel Chen-Tournoux2Patrick Farahmand3Valérie Bellamy4Gwennhael Autret5Christine Ménager6Albert Hagège7Jerome Larghéro8Florence Gazeau9Olivier Clément10Philippe Menasché11Assistance Publique-Hôpitaux de Paris, Ecole de Chirurgie, Paris, FranceUniversité Paris-Diderot, Paris, FranceAssistance Publique-Hôpitaux de Paris, Ecole de Chirurgie, Paris, FranceUniversité Paris Descartes, Sorbonne Paris Cité, Paris, FranceAssistance Publique-Hôpitaux de Paris, Ecole de Chirurgie, Paris, FranceINSERM, U970, Paris Cardiovascular Research Center-PARCC, Paris, FranceUniv Paris 06-CNRS-ESPCI Laboratoire PECSA UMR7195, Paris, FranceUniversité Paris Descartes, Sorbonne Paris Cité, Paris, FranceUniversity Paris Diderot, Paris, FranceUniversité Paris-Diderot, Paris, FranceINSERM, U970, Paris Cardiovascular Research Center-PARCC, Paris, FranceUniversité Paris Descartes, Sorbonne Paris Cité, Paris, FranceTherapeutic intracavitary stem cell infusion currently suffers from poor myocardial homing. We examined whether cardiac cell retention could be enhanced by magnetic targeting of endothelial progenitor cells (EPCs) loaded with iron oxide nanoparticles. EPCs were magnetically labeled with citrate-coated iron oxide nanoparticles. Cell proliferation, migration, and CXCR4 chemokine receptor expression were assessed in different labeling conditions and no adverse effects of the magnetic label were observed. The magnetophoretic mobility of labeled EPCs was determined in vitro, with the same magnet as that subsequently used in vivo. Coronary artery occlusion was induced for 30 min in 36 rats (31 survivors), followed by 20 min of reperfusion. The rats were randomized to receive, during brief aortic cross-clamping, direct intraventricular injection of culture medium ( n = 7) or magnetically labeled EPCs ( n = 24), with ( n = 14) or without ( n = 10) subcutaneous insertion of a magnet over the chest cavity ( n = 14). The hearts were explanted 24 h later and engrafted cells were visualized by magnetic resonance imaging (MRI) of the heart at 1.5 T. Their abundance in the myocardium was also analyzed semiquantitatively by immunofluorescence, and quantitatively by real-time polymerase chain reaction (RT-PCR). Although differences in cell retention between groups failed to be statistically significant using RT-PCR quantification, due to the variability of the animal model, immunostaining showed that the average number of engrafted EPCs was significantly ten times higher with than without magnetic targeting. There was thus a consistent trend favoring the magnet-treated hearts, thereby suggesting magnetic targeting as a potentially new mean of enhancing myocardial homing of intravascularly delivered stem cells. Magnetic targeting has the potential to enhance myocardial retention of intravascularly delivered endothelial progenitor cells.https://doi.org/10.3727/096368911X612440 |
| spellingShingle | Aurélie Chaudeurge Claire Wilhelm Annabel Chen-Tournoux Patrick Farahmand Valérie Bellamy Gwennhael Autret Christine Ménager Albert Hagège Jerome Larghéro Florence Gazeau Olivier Clément Philippe Menasché Can Magnetic Targeting of Magnetically Labeled Circulating Cells Optimize Intramyocardial Cell Retention? Cell Transplantation |
| title | Can Magnetic Targeting of Magnetically Labeled Circulating Cells Optimize Intramyocardial Cell Retention? |
| title_full | Can Magnetic Targeting of Magnetically Labeled Circulating Cells Optimize Intramyocardial Cell Retention? |
| title_fullStr | Can Magnetic Targeting of Magnetically Labeled Circulating Cells Optimize Intramyocardial Cell Retention? |
| title_full_unstemmed | Can Magnetic Targeting of Magnetically Labeled Circulating Cells Optimize Intramyocardial Cell Retention? |
| title_short | Can Magnetic Targeting of Magnetically Labeled Circulating Cells Optimize Intramyocardial Cell Retention? |
| title_sort | can magnetic targeting of magnetically labeled circulating cells optimize intramyocardial cell retention |
| url | https://doi.org/10.3727/096368911X612440 |
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