Alternative supplements and ms medium concentrations in the in vitro establishment of Dipteryx alata Vog.
Dipteryx alata Vog. (barueiro) is a native fruit of the Cerrado used in food and medicine. The species has never been domesticated and is currently at risk of extinction. Plant tissue culture is an important tool for the conservation of germplasm and for quick, large-scale propagation of quality se...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Federal de Uberlândia
2016-10-01
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| Series: | Bioscience Journal |
| Subjects: | |
| Online Access: | https://seer-dev.ufu.br/index.php/biosciencejournal/article/view/33682 |
| _version_ | 1818287885742243840 |
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| author | Herick Fernando de Jesus Silva Simone Abreu Asmar Rayssa Camargo de Oliveira José Magno Queiroz Luz Berildo de Melo |
| author_facet | Herick Fernando de Jesus Silva Simone Abreu Asmar Rayssa Camargo de Oliveira José Magno Queiroz Luz Berildo de Melo |
| author_sort | Herick Fernando de Jesus Silva |
| collection | DOAJ |
| description |
Dipteryx alata Vog. (barueiro) is a native fruit of the Cerrado used in food and medicine. The species has never been domesticated and is currently at risk of extinction. Plant tissue culture is an important tool for the conservation of germplasm and for quick, large-scale propagation of quality seedlings. While, this technique has never been used with barueiro, it could be a valuable tool in the domestication of this species. The objective of this work is to evaluate concentrations of MS medium salts (25, 50, 75 and 100%) supplemented with coconut water (100 mL L-1), dwarf banana pulp (60 g L-1) or the absence of any supplement in the in vitro establishment of barueiro seeds (Dipteryx alata Vog). The experiment used a completely randomized design (CRD) with a 3x4 factorial scheme (three cultivation media types and four concentrations of MS salts) and three repetitions. The following characteristics were evaluated: germination rate , shoot length , largest root length , stem diameter , number of leaves , chlorophyll A , chlorophyll B , total chlorophyll , and the fresh and dry mass of the seedlings. The coconut water and banana pulp supplements did not improve the in vitro development of the barueiro seedlings and the full concentration of MS salts did not improve the initial development of the seedlings. However, the pure MS medium at a concentration of 25% MS salts was the best option for the in vitro establishment of Dipteyx alata.
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| first_indexed | 2024-12-13T01:47:36Z |
| format | Article |
| id | doaj.art-8ca3607ffd804258b7e66edca67a855a |
| institution | Directory Open Access Journal |
| issn | 1981-3163 |
| language | English |
| last_indexed | 2024-12-13T01:47:36Z |
| publishDate | 2016-10-01 |
| publisher | Universidade Federal de Uberlândia |
| record_format | Article |
| series | Bioscience Journal |
| spelling | doaj.art-8ca3607ffd804258b7e66edca67a855a2022-12-22T00:03:36ZengUniversidade Federal de UberlândiaBioscience Journal1981-31632016-10-01325Alternative supplements and ms medium concentrations in the in vitro establishment of Dipteryx alata Vog. Herick Fernando de Jesus Silva0Simone Abreu Asmar1Rayssa Camargo de Oliveira2José Magno Queiroz Luz3Berildo de Melo4Federal University of UberlândiaFederal University of UberlândiaFederal University of UberlândiaFederal University of UberlândiaFederal University of Uberlândia Dipteryx alata Vog. (barueiro) is a native fruit of the Cerrado used in food and medicine. The species has never been domesticated and is currently at risk of extinction. Plant tissue culture is an important tool for the conservation of germplasm and for quick, large-scale propagation of quality seedlings. While, this technique has never been used with barueiro, it could be a valuable tool in the domestication of this species. The objective of this work is to evaluate concentrations of MS medium salts (25, 50, 75 and 100%) supplemented with coconut water (100 mL L-1), dwarf banana pulp (60 g L-1) or the absence of any supplement in the in vitro establishment of barueiro seeds (Dipteryx alata Vog). The experiment used a completely randomized design (CRD) with a 3x4 factorial scheme (three cultivation media types and four concentrations of MS salts) and three repetitions. The following characteristics were evaluated: germination rate , shoot length , largest root length , stem diameter , number of leaves , chlorophyll A , chlorophyll B , total chlorophyll , and the fresh and dry mass of the seedlings. The coconut water and banana pulp supplements did not improve the in vitro development of the barueiro seedlings and the full concentration of MS salts did not improve the initial development of the seedlings. However, the pure MS medium at a concentration of 25% MS salts was the best option for the in vitro establishment of Dipteyx alata. https://seer-dev.ufu.br/index.php/biosciencejournal/article/view/33682Natural supplementsCulture mediumBrazilian savannah fruit treeBaruzeiro |
| spellingShingle | Herick Fernando de Jesus Silva Simone Abreu Asmar Rayssa Camargo de Oliveira José Magno Queiroz Luz Berildo de Melo Alternative supplements and ms medium concentrations in the in vitro establishment of Dipteryx alata Vog. Bioscience Journal Natural supplements Culture medium Brazilian savannah fruit tree Baruzeiro |
| title | Alternative supplements and ms medium concentrations in the in vitro establishment of Dipteryx alata Vog. |
| title_full | Alternative supplements and ms medium concentrations in the in vitro establishment of Dipteryx alata Vog. |
| title_fullStr | Alternative supplements and ms medium concentrations in the in vitro establishment of Dipteryx alata Vog. |
| title_full_unstemmed | Alternative supplements and ms medium concentrations in the in vitro establishment of Dipteryx alata Vog. |
| title_short | Alternative supplements and ms medium concentrations in the in vitro establishment of Dipteryx alata Vog. |
| title_sort | alternative supplements and ms medium concentrations in the in vitro establishment of dipteryx alata vog |
| topic | Natural supplements Culture medium Brazilian savannah fruit tree Baruzeiro |
| url | https://seer-dev.ufu.br/index.php/biosciencejournal/article/view/33682 |
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