Time-resolved single-cell sequencing identifies multiple waves of mRNA decay during the mitosis-to-G1 phase transition
Accurate control of the cell cycle is critical for development and tissue homeostasis, and requires precisely timed expression of many genes. Cell cycle gene expression is regulated through transcriptional and translational control, as well as through regulated protein degradation. Here, we show tha...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2022-02-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/71356 |
| _version_ | 1828058377234677760 |
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| author | Lenno Krenning Stijn Sonneveld Marvin E Tanenbaum |
| author_facet | Lenno Krenning Stijn Sonneveld Marvin E Tanenbaum |
| author_sort | Lenno Krenning |
| collection | DOAJ |
| description | Accurate control of the cell cycle is critical for development and tissue homeostasis, and requires precisely timed expression of many genes. Cell cycle gene expression is regulated through transcriptional and translational control, as well as through regulated protein degradation. Here, we show that widespread and temporally controlled mRNA decay acts as an additional mechanism for gene expression regulation during the cell cycle in human cells. We find that two waves of mRNA decay occur sequentially during the mitosis-to-G1 phase transition, and we identify the deadenylase CNOT1 as a factor that contributes to mRNA decay during this cell cycle transition. Collectively, our data show that, akin to protein degradation, scheduled mRNA decay helps to reshape cell cycle gene expression as cells move from mitosis into G1 phase. |
| first_indexed | 2024-04-10T21:26:16Z |
| format | Article |
| id | doaj.art-8ca49871880847cab83e13eda6ea4597 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-10T21:26:16Z |
| publishDate | 2022-02-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-8ca49871880847cab83e13eda6ea45972023-01-19T16:34:05ZengeLife Sciences Publications LtdeLife2050-084X2022-02-011110.7554/eLife.71356Time-resolved single-cell sequencing identifies multiple waves of mRNA decay during the mitosis-to-G1 phase transitionLenno Krenning0https://orcid.org/0000-0001-6696-0512Stijn Sonneveld1Marvin E Tanenbaum2https://orcid.org/0000-0001-8762-0090Oncode Institute, Hubrecht Institute – KNAW and University Medical Center Utrecht, Utrecht, NetherlandsOncode Institute, Hubrecht Institute – KNAW and University Medical Center Utrecht, Utrecht, NetherlandsOncode Institute, Hubrecht Institute – KNAW and University Medical Center Utrecht, Utrecht, NetherlandsAccurate control of the cell cycle is critical for development and tissue homeostasis, and requires precisely timed expression of many genes. Cell cycle gene expression is regulated through transcriptional and translational control, as well as through regulated protein degradation. Here, we show that widespread and temporally controlled mRNA decay acts as an additional mechanism for gene expression regulation during the cell cycle in human cells. We find that two waves of mRNA decay occur sequentially during the mitosis-to-G1 phase transition, and we identify the deadenylase CNOT1 as a factor that contributes to mRNA decay during this cell cycle transition. Collectively, our data show that, akin to protein degradation, scheduled mRNA decay helps to reshape cell cycle gene expression as cells move from mitosis into G1 phase.https://elifesciences.org/articles/71356mRNA decaycell cyclegene regulationsingle-cell sequencing |
| spellingShingle | Lenno Krenning Stijn Sonneveld Marvin E Tanenbaum Time-resolved single-cell sequencing identifies multiple waves of mRNA decay during the mitosis-to-G1 phase transition eLife mRNA decay cell cycle gene regulation single-cell sequencing |
| title | Time-resolved single-cell sequencing identifies multiple waves of mRNA decay during the mitosis-to-G1 phase transition |
| title_full | Time-resolved single-cell sequencing identifies multiple waves of mRNA decay during the mitosis-to-G1 phase transition |
| title_fullStr | Time-resolved single-cell sequencing identifies multiple waves of mRNA decay during the mitosis-to-G1 phase transition |
| title_full_unstemmed | Time-resolved single-cell sequencing identifies multiple waves of mRNA decay during the mitosis-to-G1 phase transition |
| title_short | Time-resolved single-cell sequencing identifies multiple waves of mRNA decay during the mitosis-to-G1 phase transition |
| title_sort | time resolved single cell sequencing identifies multiple waves of mrna decay during the mitosis to g1 phase transition |
| topic | mRNA decay cell cycle gene regulation single-cell sequencing |
| url | https://elifesciences.org/articles/71356 |
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