Effect of long-term fertilization strategies on bacterial community composition in a 35-year field experiment of Chinese Mollisols
Abstract Bacteria play vital roles in soil biological fertility; however, it remains poorly understood about their response to long-term fertilization in Chinese Mollisols, especially when organic manure is substituted for inorganic nitrogen (N) fertilizer. To broaden our knowledge, high-throughput...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2018-02-01
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| Series: | AMB Express |
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| Online Access: | http://link.springer.com/article/10.1186/s13568-018-0549-8 |
| _version_ | 1819055342660616192 |
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| author | Mingchao Ma Jing Zhou Marc Ongena Wenzheng Liu Dan Wei Baisuo Zhao Dawei Guan Xin Jiang Jun Li |
| author_facet | Mingchao Ma Jing Zhou Marc Ongena Wenzheng Liu Dan Wei Baisuo Zhao Dawei Guan Xin Jiang Jun Li |
| author_sort | Mingchao Ma |
| collection | DOAJ |
| description | Abstract Bacteria play vital roles in soil biological fertility; however, it remains poorly understood about their response to long-term fertilization in Chinese Mollisols, especially when organic manure is substituted for inorganic nitrogen (N) fertilizer. To broaden our knowledge, high-throughput pyrosequencing and quantitative PCR were used to explore the impacts of inorganic fertilizer and manure on bacterial community composition in a 35-year field experiment of Chinese Mollisols. Soils were collected from four treatments: no fertilizer (CK), inorganic phosphorus (P) and potassium (K) fertilizer (PK), inorganic P, K, and N fertilizer (NPK), and inorganic P and K fertilizer plus manure (MPK). All fertilization differently changed soil properties. Compared with CK, the PK and NPK treatments acidified soil by significantly decreasing soil pH from 6.48 to 5.53 and 6.16, respectively, while MPK application showed no significant differences of soil pH, indicating alleviation of soil acidification. Moreover, all fertilization significantly increased soil organic matter (OM) and soybean yields, with the highest observed under MPK regime. In addition, the community composition at each taxonomic level varied considerably among the fertilization strategies. Bacterial taxa, associated with plant growth promotion, OM accumulation, disease suppression, and increased soil enzyme activity, were overrepresented in the MPK regime, while they were present at low abundant levels under NPK treatment, i.e. phyla Proteobacteria and Bacteroidetes, class Alphaproteobacteria, and genera Variovorax, Chthoniobacter, Massilia, Lysobacter, Catelliglobosispora and Steroidobacter. The application of MPK shifted soil bacterial community composition towards a better status, and such shifts were primarily derived from changes in soil pH and OM. |
| first_indexed | 2024-12-21T13:06:00Z |
| format | Article |
| id | doaj.art-8ca569ec4cfd45de92b6cd30d50d21ab |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-21T13:06:00Z |
| publishDate | 2018-02-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-8ca569ec4cfd45de92b6cd30d50d21ab2022-12-21T19:03:02ZengSpringerOpenAMB Express2191-08552018-02-018111110.1186/s13568-018-0549-8Effect of long-term fertilization strategies on bacterial community composition in a 35-year field experiment of Chinese MollisolsMingchao Ma0Jing Zhou1Marc Ongena2Wenzheng Liu3Dan Wei4Baisuo Zhao5Dawei Guan6Xin Jiang7Jun Li8Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural SciencesInstitute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural SciencesMicrobial Processes and Interactions Research Unit, Gembloux Agro-Bio Tech, University of LiègeMicrobial Processes and Interactions Research Unit, Gembloux Agro-Bio Tech, University of LiègeThe Institute of Soil Fertility and Environmental Sources, Heilongjiang Academy of Agricultural SciencesLaboratory of Quality & Safety Risk Assessment for Microbial Products, Ministry of AgricultureInstitute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural SciencesInstitute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural SciencesInstitute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural SciencesAbstract Bacteria play vital roles in soil biological fertility; however, it remains poorly understood about their response to long-term fertilization in Chinese Mollisols, especially when organic manure is substituted for inorganic nitrogen (N) fertilizer. To broaden our knowledge, high-throughput pyrosequencing and quantitative PCR were used to explore the impacts of inorganic fertilizer and manure on bacterial community composition in a 35-year field experiment of Chinese Mollisols. Soils were collected from four treatments: no fertilizer (CK), inorganic phosphorus (P) and potassium (K) fertilizer (PK), inorganic P, K, and N fertilizer (NPK), and inorganic P and K fertilizer plus manure (MPK). All fertilization differently changed soil properties. Compared with CK, the PK and NPK treatments acidified soil by significantly decreasing soil pH from 6.48 to 5.53 and 6.16, respectively, while MPK application showed no significant differences of soil pH, indicating alleviation of soil acidification. Moreover, all fertilization significantly increased soil organic matter (OM) and soybean yields, with the highest observed under MPK regime. In addition, the community composition at each taxonomic level varied considerably among the fertilization strategies. Bacterial taxa, associated with plant growth promotion, OM accumulation, disease suppression, and increased soil enzyme activity, were overrepresented in the MPK regime, while they were present at low abundant levels under NPK treatment, i.e. phyla Proteobacteria and Bacteroidetes, class Alphaproteobacteria, and genera Variovorax, Chthoniobacter, Massilia, Lysobacter, Catelliglobosispora and Steroidobacter. The application of MPK shifted soil bacterial community composition towards a better status, and such shifts were primarily derived from changes in soil pH and OM.http://link.springer.com/article/10.1186/s13568-018-0549-8Soil degradationFertilizationBacterial communityIllumina MiSeqqPCR |
| spellingShingle | Mingchao Ma Jing Zhou Marc Ongena Wenzheng Liu Dan Wei Baisuo Zhao Dawei Guan Xin Jiang Jun Li Effect of long-term fertilization strategies on bacterial community composition in a 35-year field experiment of Chinese Mollisols AMB Express Soil degradation Fertilization Bacterial community Illumina MiSeq qPCR |
| title | Effect of long-term fertilization strategies on bacterial community composition in a 35-year field experiment of Chinese Mollisols |
| title_full | Effect of long-term fertilization strategies on bacterial community composition in a 35-year field experiment of Chinese Mollisols |
| title_fullStr | Effect of long-term fertilization strategies on bacterial community composition in a 35-year field experiment of Chinese Mollisols |
| title_full_unstemmed | Effect of long-term fertilization strategies on bacterial community composition in a 35-year field experiment of Chinese Mollisols |
| title_short | Effect of long-term fertilization strategies on bacterial community composition in a 35-year field experiment of Chinese Mollisols |
| title_sort | effect of long term fertilization strategies on bacterial community composition in a 35 year field experiment of chinese mollisols |
| topic | Soil degradation Fertilization Bacterial community Illumina MiSeq qPCR |
| url | http://link.springer.com/article/10.1186/s13568-018-0549-8 |
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