''Neurodegeneration and Oxidative Stress in Brain Tissues Induced by Tramadol with the Protective Effects of Royal Jelly in Rats''.
Tramadol hydrochloride (TH) is an opioid centrally acting analgesic used to treat moderate to severe acute and chronic pains. Therefore, it became the most prescribed opioid worldwide.In this study, we investigated the neurodegenerative disorders of tramadol in brain tissues and the protective role...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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South Valley University
2023-09-01
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| Series: | SVU-International Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | https://svu.journals.ekb.eg/article_317622.html |
| _version_ | 1797663074441756672 |
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| author | Atef M. Khalil Amany Sayed Mowas Mohammed H. Hassan Hassan Ahmed Obeid Shanab |
| author_facet | Atef M. Khalil Amany Sayed Mowas Mohammed H. Hassan Hassan Ahmed Obeid Shanab |
| author_sort | Atef M. Khalil |
| collection | DOAJ |
| description | Tramadol hydrochloride (TH) is an opioid centrally acting analgesic used to treat moderate to severe acute and chronic pains. Therefore, it became the most prescribed opioid worldwide.In this study, we investigated the neurodegenerative disorders of tramadol in brain tissues and the protective role of royal jelly. Twenty male albino rats allocated into four groups: Group 1,served as a control group, and Group 2, administrated with tramadol at a dose of 20 mg/kg/b. W for 60 days. Group 3: rats administrated with tramadol at a dose of 20 mg/kg/b. W for 60 days and treated with royal jelly (RJ) in a 100 mg/kg dose. b.w. Group 4: Rats inoculated with royal jelly (RJ) at a dose of 100 mg/kg. b.w. Blood samples were collected for hematological and biochemical analysis. Brain tissues were harvested for neurodegeneration biomarkers detection and histopathological examinations. Administration of tramadol revealed a significant decrease in Hb concentration, RBCs count, PCV %, Lymphocytes %, and platelets number, while WBCS count, Neutrophiles, and monocytes % increased. Also, Tramadol induced a decrease in glucose-6phosphate dehydrogenase (G6PD) while creatine kinase -BB (CK-BB) and neuron-specific enolase enzymes (NSE) were decreased.Tramadol increased the lipid peroxidation MDA, while total antioxidants capacity (TAC) and glutathione reductase (GSH) concentrations were decreased. Histopathologically, tramadol-induced neurodegenerative changes in brain neurons manifested by acute necrosed neurons with gliosis and vascular congestions. The administration of royal jelly improved the previous deleterious effects by decreasing brain tissue oxidative stress. Tramadol misuse caused neurodegenerative effects and was relieved by RJ administration. |
| first_indexed | 2024-03-11T19:09:16Z |
| format | Article |
| id | doaj.art-8ca759c9926348de8204c8fd53ce66a4 |
| institution | Directory Open Access Journal |
| issn | 2535-1826 2535-1877 |
| language | English |
| last_indexed | 2024-03-11T19:09:16Z |
| publishDate | 2023-09-01 |
| publisher | South Valley University |
| record_format | Article |
| series | SVU-International Journal of Veterinary Sciences |
| spelling | doaj.art-8ca759c9926348de8204c8fd53ce66a42023-10-09T20:39:26ZengSouth Valley UniversitySVU-International Journal of Veterinary Sciences2535-18262535-18772023-09-016311412910.21608/SVU.2023.215343.1276''Neurodegeneration and Oxidative Stress in Brain Tissues Induced by Tramadol with the Protective Effects of Royal Jelly in Rats''.Atef M. Khalil0Amany Sayed Mowas1Mohammed H. Hassan2Hassan Ahmed3Obeid Shanab4 Department of Pathology and Clinical Pathology, Faculty of Veterinary Medicine, South Valley University, 83523 Qena, Egypt Department of Pathology and Clinical Pathology, Faculty of Veterinary Medicine, South Valley University, 83523 Qena, EgyptDepartment of Medical Biochemistry, Faculty of Medicine, South Valley University, 83523 Qena, EgyptDepartment of Physiology, Faculty of Veterinary Medicine, South Valley University, 83523 Qena, EgyptDepartment of Biochemistry, Faculty of Veterinary Medicine, South Valley University, Qena 83523, EgyptTramadol hydrochloride (TH) is an opioid centrally acting analgesic used to treat moderate to severe acute and chronic pains. Therefore, it became the most prescribed opioid worldwide.In this study, we investigated the neurodegenerative disorders of tramadol in brain tissues and the protective role of royal jelly. Twenty male albino rats allocated into four groups: Group 1,served as a control group, and Group 2, administrated with tramadol at a dose of 20 mg/kg/b. W for 60 days. Group 3: rats administrated with tramadol at a dose of 20 mg/kg/b. W for 60 days and treated with royal jelly (RJ) in a 100 mg/kg dose. b.w. Group 4: Rats inoculated with royal jelly (RJ) at a dose of 100 mg/kg. b.w. Blood samples were collected for hematological and biochemical analysis. Brain tissues were harvested for neurodegeneration biomarkers detection and histopathological examinations. Administration of tramadol revealed a significant decrease in Hb concentration, RBCs count, PCV %, Lymphocytes %, and platelets number, while WBCS count, Neutrophiles, and monocytes % increased. Also, Tramadol induced a decrease in glucose-6phosphate dehydrogenase (G6PD) while creatine kinase -BB (CK-BB) and neuron-specific enolase enzymes (NSE) were decreased.Tramadol increased the lipid peroxidation MDA, while total antioxidants capacity (TAC) and glutathione reductase (GSH) concentrations were decreased. Histopathologically, tramadol-induced neurodegenerative changes in brain neurons manifested by acute necrosed neurons with gliosis and vascular congestions. The administration of royal jelly improved the previous deleterious effects by decreasing brain tissue oxidative stress. Tramadol misuse caused neurodegenerative effects and was relieved by RJ administration. https://svu.journals.ekb.eg/article_317622.htmltramadolroyal jellyoxidative stressbrainneurodegenerationmisuse |
| spellingShingle | Atef M. Khalil Amany Sayed Mowas Mohammed H. Hassan Hassan Ahmed Obeid Shanab ''Neurodegeneration and Oxidative Stress in Brain Tissues Induced by Tramadol with the Protective Effects of Royal Jelly in Rats''. SVU-International Journal of Veterinary Sciences tramadol royal jelly oxidative stress brain neurodegeneration misuse |
| title | ''Neurodegeneration and Oxidative Stress in Brain Tissues Induced by Tramadol with the Protective Effects of Royal Jelly in Rats''. |
| title_full | ''Neurodegeneration and Oxidative Stress in Brain Tissues Induced by Tramadol with the Protective Effects of Royal Jelly in Rats''. |
| title_fullStr | ''Neurodegeneration and Oxidative Stress in Brain Tissues Induced by Tramadol with the Protective Effects of Royal Jelly in Rats''. |
| title_full_unstemmed | ''Neurodegeneration and Oxidative Stress in Brain Tissues Induced by Tramadol with the Protective Effects of Royal Jelly in Rats''. |
| title_short | ''Neurodegeneration and Oxidative Stress in Brain Tissues Induced by Tramadol with the Protective Effects of Royal Jelly in Rats''. |
| title_sort | neurodegeneration and oxidative stress in brain tissues induced by tramadol with the protective effects of royal jelly in rats |
| topic | tramadol royal jelly oxidative stress brain neurodegeneration misuse |
| url | https://svu.journals.ekb.eg/article_317622.html |
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