Study of Mesenchymal Stem Cell Proliferation and Bone Differentiation on Composite Scaffolds of PLLA and Nano Hydroxyapatite with Different Morphologies
Objective: Nowadays, bone constructs elaborated according to tissue engineering principlesare being regarded as an ideal choice for the reconstruction of segmental bonedefects. In this study, proliferation and bone differentiation of marrow-derived mesenchymalstem cells (MSCs) were compared in diffe...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Royan Institute (ACECR), Tehran
2011-01-01
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| Series: | Cell Journal |
| Subjects: | |
| Online Access: | http://celljournal.org/library/upload/article/af_2222332Baghban%20Eslaminejad.pdf |
| Summary: | Objective: Nowadays, bone constructs elaborated according to tissue engineering principlesare being regarded as an ideal choice for the reconstruction of segmental bonedefects. In this study, proliferation and bone differentiation of marrow-derived mesenchymalstem cells (MSCs) were compared in different composite scaffolds containing varyingmorphologies of nano hydroxyapatite (nHAP).Materials and Methods: Needle nHAP/PLLA (poly (L-lactide acid)), spherical nHAP/PLLA and rod nHAP/PLLA scaffolds were prepared and 3D cultures of passaged-3 ratMSCs were established using the scaffolds. The loading of the cells onto the scaffoldinternal spaces was confirmed with microscopy and their proliferation was determined byMTT assay. To compare the osteogenic differentiation of the cells on the scaffold surfaces,osteogenic 3D cultures were established and kept for 21 days. At the end of this periodculture mineralization and relative bone-related gene expression were quantified usingthe alizarin red quantification assay and semi quantitative RT-PCR analysis respectively.ANOVA was used to compare the data.Results: According to the MTT assays, cells adhered to all the studied scaffold surfacestended to proliferate. In this respect the microenvironment provided by the needle nHAP/PLLA appeared much better than that of either the spherical or rod nHAP/PLLA scaffolds(P<0.05). Similarly, mineralization was observed to be heavier for the needle nHAP/PLLAscaffold compared to the two other composite scaffolds. In addition, the relative expressionof coll I, osteocalcin, runx2 and ALP genes all appeared to be significantly higher in the cellscultivated on needle nHAP/PLLA scaffolds versus their spherical and rod counterparts.Conclusion: Overall, needle nHAP/PLLA scaffolds appear to provide the most appropriatematrix for producing bone construct using MSCs. |
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| ISSN: | 2228-5806 2228-5814 |