Production, bioprocess optimization and γ-irradiation of Penicillium polonicum, as a new Taxol producing endophyte from Ginko biloba
Twenty-eight fungal endophytes were recovered from the different parts of Ginkgo biloba and screened for their Taxol producing potency. Among these isolates, Penicillium polonicum AUMC14487 was reported as the potent Taxol producer (90.53 μg/l). The chemical identity of the extracted Taxol was verif...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-06-01
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| Series: | Biotechnology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X21000394 |
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| author | Sobhy S. Abdel-Fatah Ahmed I. El-Batal Gamal M. El-Sherbiny Mahmoud A. Khalaf Ashraf S. El-Sayed |
| author_facet | Sobhy S. Abdel-Fatah Ahmed I. El-Batal Gamal M. El-Sherbiny Mahmoud A. Khalaf Ashraf S. El-Sayed |
| author_sort | Sobhy S. Abdel-Fatah |
| collection | DOAJ |
| description | Twenty-eight fungal endophytes were recovered from the different parts of Ginkgo biloba and screened for their Taxol producing potency. Among these isolates, Penicillium polonicum AUMC14487 was reported as the potent Taxol producer (90.53 μg/l). The chemical identity of the extracted Taxol was verified from the TLC, HPLC, NMR, EDX, and FTIR analyses. The extracted Taxol displayed a strong antiproliferative activity against HEPG2 (IC50 4.06 μM) and MCF7 (IC50 6.07 μM). The yield of Taxol by P. polonicum was optimized by nutritional optimization with the Response Surface Methodology (RSM) using Plackett-Burman and Central Composite Designs. In addition to nutritional optimization, the effect of γ-irradiation of the spores of P. polonicum on its Taxol producing potency was evaluated. The yield of Taxol by P. polonicum was increased via nutritional optimization by response surface methodology with Plackett-Burman and FCCD designs, and γ-irradiation by about 4.5 folds, comparing to the control culture.The yield of Taxol was increased by about 1.2 folds (401.2 μg/l) by γ -irradiation of the isolates at 0.5−0.75 kGy, comparing to the control cultures (332.2 μg/l).The highest Taxol yield was obtained by growing P. polonicum on modified Czapek’s- Dox medium (sucrose 40.0 g/l, malt extract 20.0 g/l, peptone 2.0 g/l, K2PO4 2.0 g/l, KCl 1.0 g/l, NaNO3 2.0 g/l, MgSO4. 5H2O 1.0 g/l) of pH 7.0 at 30.0 °C for 7.0 days. From the FCCD design, sucrose, malt extract and incubation time being the highest significant variables medium components affecting the Taxol production by P. polonicum. |
| first_indexed | 2024-12-15T00:38:01Z |
| format | Article |
| id | doaj.art-8cae58bdce0c445d9b957fb115cd9439 |
| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-12-15T00:38:01Z |
| publishDate | 2021-06-01 |
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| series | Biotechnology Reports |
| spelling | doaj.art-8cae58bdce0c445d9b957fb115cd94392022-12-21T22:41:45ZengElsevierBiotechnology Reports2215-017X2021-06-0130e00623Production, bioprocess optimization and γ-irradiation of Penicillium polonicum, as a new Taxol producing endophyte from Ginko bilobaSobhy S. Abdel-Fatah0Ahmed I. El-Batal1Gamal M. El-Sherbiny2Mahmoud A. Khalaf3Ashraf S. El-Sayed4Drug Radiation Research Dep., Biotechnology Division, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority, Cairo, EgyptDrug Radiation Research Dep., Biotechnology Division, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority, Cairo, EgyptBotany and Microbiology Department, Faculty of Science (Boys), Al-Azhar University, Cairo, EgyptDrug Radiation Research Dep., Biotechnology Division, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority, Cairo, Egypt; Microbiology Dep., Biotechnology Division, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority, Cairo, EgyptEnzymology and Fungal Biotechnology Lab (EFBL), Botany and Microbiology Department, Faculty of Science, Zagazig University, 44519, Egypt; Corresponding author.Twenty-eight fungal endophytes were recovered from the different parts of Ginkgo biloba and screened for their Taxol producing potency. Among these isolates, Penicillium polonicum AUMC14487 was reported as the potent Taxol producer (90.53 μg/l). The chemical identity of the extracted Taxol was verified from the TLC, HPLC, NMR, EDX, and FTIR analyses. The extracted Taxol displayed a strong antiproliferative activity against HEPG2 (IC50 4.06 μM) and MCF7 (IC50 6.07 μM). The yield of Taxol by P. polonicum was optimized by nutritional optimization with the Response Surface Methodology (RSM) using Plackett-Burman and Central Composite Designs. In addition to nutritional optimization, the effect of γ-irradiation of the spores of P. polonicum on its Taxol producing potency was evaluated. The yield of Taxol by P. polonicum was increased via nutritional optimization by response surface methodology with Plackett-Burman and FCCD designs, and γ-irradiation by about 4.5 folds, comparing to the control culture.The yield of Taxol was increased by about 1.2 folds (401.2 μg/l) by γ -irradiation of the isolates at 0.5−0.75 kGy, comparing to the control cultures (332.2 μg/l).The highest Taxol yield was obtained by growing P. polonicum on modified Czapek’s- Dox medium (sucrose 40.0 g/l, malt extract 20.0 g/l, peptone 2.0 g/l, K2PO4 2.0 g/l, KCl 1.0 g/l, NaNO3 2.0 g/l, MgSO4. 5H2O 1.0 g/l) of pH 7.0 at 30.0 °C for 7.0 days. From the FCCD design, sucrose, malt extract and incubation time being the highest significant variables medium components affecting the Taxol production by P. polonicum.http://www.sciencedirect.com/science/article/pii/S2215017X21000394Ginko bilobaEndophytic fungiPenicillium polonicumTaxolOptimizationPlacket-Burman design |
| spellingShingle | Sobhy S. Abdel-Fatah Ahmed I. El-Batal Gamal M. El-Sherbiny Mahmoud A. Khalaf Ashraf S. El-Sayed Production, bioprocess optimization and γ-irradiation of Penicillium polonicum, as a new Taxol producing endophyte from Ginko biloba Biotechnology Reports Ginko biloba Endophytic fungi Penicillium polonicum Taxol Optimization Placket-Burman design |
| title | Production, bioprocess optimization and γ-irradiation of Penicillium polonicum, as a new Taxol producing endophyte from Ginko biloba |
| title_full | Production, bioprocess optimization and γ-irradiation of Penicillium polonicum, as a new Taxol producing endophyte from Ginko biloba |
| title_fullStr | Production, bioprocess optimization and γ-irradiation of Penicillium polonicum, as a new Taxol producing endophyte from Ginko biloba |
| title_full_unstemmed | Production, bioprocess optimization and γ-irradiation of Penicillium polonicum, as a new Taxol producing endophyte from Ginko biloba |
| title_short | Production, bioprocess optimization and γ-irradiation of Penicillium polonicum, as a new Taxol producing endophyte from Ginko biloba |
| title_sort | production bioprocess optimization and γ irradiation of penicillium polonicum as a new taxol producing endophyte from ginko biloba |
| topic | Ginko biloba Endophytic fungi Penicillium polonicum Taxol Optimization Placket-Burman design |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X21000394 |
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