Tissue-Wide Expression of Genes Related to Vitamin D Metabolism and FGF23 Signaling following Variable Phosphorus Intake in Pigs
Calcium (Ca) and phosphorus (P) homeostasis is maintained by several regulators, including vitamin D and fibroblast growth factor 23 (FGF23), and their tissue-specific activation and signaling cascades. In this study, the tissue-wide expression of key genes linked to vitamin D metabolism (<i>C...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-08-01
|
| Series: | Metabolites |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-1989/12/8/729 |
| _version_ | 1827626536336883712 |
|---|---|
| author | Maruf Hasan Michael Oster Henry Reyer Siriluck Ponsuksili Eduard Murani Petra Wolf Dagmar-Christiane Fischer Klaus Wimmers |
| author_facet | Maruf Hasan Michael Oster Henry Reyer Siriluck Ponsuksili Eduard Murani Petra Wolf Dagmar-Christiane Fischer Klaus Wimmers |
| author_sort | Maruf Hasan |
| collection | DOAJ |
| description | Calcium (Ca) and phosphorus (P) homeostasis is maintained by several regulators, including vitamin D and fibroblast growth factor 23 (FGF23), and their tissue-specific activation and signaling cascades. In this study, the tissue-wide expression of key genes linked to vitamin D metabolism (<i>CYP2R1</i>, <i>CYP27A1</i>, <i>CYP27B1</i>, <i>CYP24A1</i>, <i>GC</i>, <i>VDR</i>) and FGF23 signaling (<i>FGF23</i>, <i>FGFR1-4</i>, <i>KL</i>) were investigated in pigs fed conventional (trial 1) and divergent P diets (trial 2). The tissue set comprised kidney, liver, bone, lung, aorta, and gastrointestinal tract sections. Expression patterns revealed that non-renal tissues and cells (NRTC) express genes to form active vitamin D [1,25(OH)<sub>2</sub>D<sub>3</sub>] according to site-specific requirements. A low P diet resulted in higher serum calcitriol and increased <i>CYP24A1</i> expression in the small intestine, indicating local suppression of vitamin D signaling. A high P diet prompted increased mRNA abundances of <i>CYP27B1</i> for local vitamin D synthesis, specifically in bone. For FGF23 signaling, analyses revealed ubiquitous expression of <i>FGFR1-4</i>, whereas <i>KL</i> was expressed in a tissue-specific manner. Dietary P supply did not affect skeletal <i>FGF23</i>; however, <i>FGFR4</i> and <i>KL</i> showed increased expression in bone at high P supply, suggesting regulation to balance mineralization. Specific NRTC responses influence vitamin D metabolism and P homeostasis, which should be considered for a thrifty but healthy P supply. |
| first_indexed | 2024-03-09T12:57:59Z |
| format | Article |
| id | doaj.art-8cb03ce2800742aa9c3427d8a085f5ed |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-09T12:57:59Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-8cb03ce2800742aa9c3427d8a085f5ed2023-11-30T21:58:12ZengMDPI AGMetabolites2218-19892022-08-0112872910.3390/metabo12080729Tissue-Wide Expression of Genes Related to Vitamin D Metabolism and FGF23 Signaling following Variable Phosphorus Intake in PigsMaruf Hasan0Michael Oster1Henry Reyer2Siriluck Ponsuksili3Eduard Murani4Petra Wolf5Dagmar-Christiane Fischer6Klaus Wimmers7Research Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, GermanyResearch Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, GermanyResearch Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, GermanyResearch Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, GermanyResearch Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, GermanyFaculty of Agricultural and Environmental Sciences, University of Rostock, Justus-von-Liebig-Weg 6b, 18059 Rostock, GermanyDepartment of Pediatrics, Rostock University Hospital, Ernst-Heydemann-Str. 8, 18057 Rostock, GermanyResearch Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, GermanyCalcium (Ca) and phosphorus (P) homeostasis is maintained by several regulators, including vitamin D and fibroblast growth factor 23 (FGF23), and their tissue-specific activation and signaling cascades. In this study, the tissue-wide expression of key genes linked to vitamin D metabolism (<i>CYP2R1</i>, <i>CYP27A1</i>, <i>CYP27B1</i>, <i>CYP24A1</i>, <i>GC</i>, <i>VDR</i>) and FGF23 signaling (<i>FGF23</i>, <i>FGFR1-4</i>, <i>KL</i>) were investigated in pigs fed conventional (trial 1) and divergent P diets (trial 2). The tissue set comprised kidney, liver, bone, lung, aorta, and gastrointestinal tract sections. Expression patterns revealed that non-renal tissues and cells (NRTC) express genes to form active vitamin D [1,25(OH)<sub>2</sub>D<sub>3</sub>] according to site-specific requirements. A low P diet resulted in higher serum calcitriol and increased <i>CYP24A1</i> expression in the small intestine, indicating local suppression of vitamin D signaling. A high P diet prompted increased mRNA abundances of <i>CYP27B1</i> for local vitamin D synthesis, specifically in bone. For FGF23 signaling, analyses revealed ubiquitous expression of <i>FGFR1-4</i>, whereas <i>KL</i> was expressed in a tissue-specific manner. Dietary P supply did not affect skeletal <i>FGF23</i>; however, <i>FGFR4</i> and <i>KL</i> showed increased expression in bone at high P supply, suggesting regulation to balance mineralization. Specific NRTC responses influence vitamin D metabolism and P homeostasis, which should be considered for a thrifty but healthy P supply.https://www.mdpi.com/2218-1989/12/8/729animal healthgene expressionmineral homeostasisnon-renal calcitriol productionpigstissue specificity |
| spellingShingle | Maruf Hasan Michael Oster Henry Reyer Siriluck Ponsuksili Eduard Murani Petra Wolf Dagmar-Christiane Fischer Klaus Wimmers Tissue-Wide Expression of Genes Related to Vitamin D Metabolism and FGF23 Signaling following Variable Phosphorus Intake in Pigs Metabolites animal health gene expression mineral homeostasis non-renal calcitriol production pigs tissue specificity |
| title | Tissue-Wide Expression of Genes Related to Vitamin D Metabolism and FGF23 Signaling following Variable Phosphorus Intake in Pigs |
| title_full | Tissue-Wide Expression of Genes Related to Vitamin D Metabolism and FGF23 Signaling following Variable Phosphorus Intake in Pigs |
| title_fullStr | Tissue-Wide Expression of Genes Related to Vitamin D Metabolism and FGF23 Signaling following Variable Phosphorus Intake in Pigs |
| title_full_unstemmed | Tissue-Wide Expression of Genes Related to Vitamin D Metabolism and FGF23 Signaling following Variable Phosphorus Intake in Pigs |
| title_short | Tissue-Wide Expression of Genes Related to Vitamin D Metabolism and FGF23 Signaling following Variable Phosphorus Intake in Pigs |
| title_sort | tissue wide expression of genes related to vitamin d metabolism and fgf23 signaling following variable phosphorus intake in pigs |
| topic | animal health gene expression mineral homeostasis non-renal calcitriol production pigs tissue specificity |
| url | https://www.mdpi.com/2218-1989/12/8/729 |
| work_keys_str_mv | AT marufhasan tissuewideexpressionofgenesrelatedtovitamindmetabolismandfgf23signalingfollowingvariablephosphorusintakeinpigs AT michaeloster tissuewideexpressionofgenesrelatedtovitamindmetabolismandfgf23signalingfollowingvariablephosphorusintakeinpigs AT henryreyer tissuewideexpressionofgenesrelatedtovitamindmetabolismandfgf23signalingfollowingvariablephosphorusintakeinpigs AT siriluckponsuksili tissuewideexpressionofgenesrelatedtovitamindmetabolismandfgf23signalingfollowingvariablephosphorusintakeinpigs AT eduardmurani tissuewideexpressionofgenesrelatedtovitamindmetabolismandfgf23signalingfollowingvariablephosphorusintakeinpigs AT petrawolf tissuewideexpressionofgenesrelatedtovitamindmetabolismandfgf23signalingfollowingvariablephosphorusintakeinpigs AT dagmarchristianefischer tissuewideexpressionofgenesrelatedtovitamindmetabolismandfgf23signalingfollowingvariablephosphorusintakeinpigs AT klauswimmers tissuewideexpressionofgenesrelatedtovitamindmetabolismandfgf23signalingfollowingvariablephosphorusintakeinpigs |