The Molecular Mechanism of Yam Polysaccharide Protected H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Damage in IEC-6 Cells
Oxidative stress is involved in maintaining homeostasis of the body, and an in-depth study of its mechanism of action is beneficial for the prevention of chronic illnesses. This study aimed to investigate the protective mechanism of yam polysaccharide (CYP) against H<sub>2</sub>O<sub&...
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| Format: | Article |
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MDPI AG
2023-01-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/2/262 |
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| author | Mingyue Shen Ruixin Cai Zhedong Li Xiaodie Chen Jianhua Xie |
| author_facet | Mingyue Shen Ruixin Cai Zhedong Li Xiaodie Chen Jianhua Xie |
| author_sort | Mingyue Shen |
| collection | DOAJ |
| description | Oxidative stress is involved in maintaining homeostasis of the body, and an in-depth study of its mechanism of action is beneficial for the prevention of chronic illnesses. This study aimed to investigate the protective mechanism of yam polysaccharide (CYP) against H<sub>2</sub>O<sub>2</sub>-induced oxidative damage by an RNA-seq technique. The expression of genes and the function of the genome in the process of oxidative damage by H<sub>2</sub>O<sub>2</sub> in IEC-6 cells were explored through transcriptomic analysis. The results illustrated that H<sub>2</sub>O<sub>2</sub> damaged cells by promoting cell differentiation and affecting tight junction proteins, and CYP could achieve cell protection via restraining the activation of the MAPK signaling pathway. RNA-seq analysis revealed that H<sub>2</sub>O<sub>2</sub> may damage cells by promoting the IL-17 signaling pathway and the MAPK signaling pathway and so forth. The Western blot showed that the pretreatment of CYP could restrain the activation of the MAPK signaling pathway. In summary, this study demonstrates that the efficacy of CYP in modulating the MAPK signaling pathway against excessive oxidative stress, with a corresponding preventive role against injury to the intestinal barrier. It provides a new perspective for the understanding of the preventive role of CYP on intestinal damage. These findings suggest that CYP could be used as oxidation protectant and may have potential application prospects in the food and pharmaceutical industries. |
| first_indexed | 2024-03-09T12:45:01Z |
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| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T12:45:01Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-8cb2f7b1a3204434a2ab9056fce8cc5f2023-11-30T22:13:51ZengMDPI AGFoods2304-81582023-01-0112226210.3390/foods12020262The Molecular Mechanism of Yam Polysaccharide Protected H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Damage in IEC-6 CellsMingyue Shen0Ruixin Cai1Zhedong Li2Xiaodie Chen3Jianhua Xie4State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaOxidative stress is involved in maintaining homeostasis of the body, and an in-depth study of its mechanism of action is beneficial for the prevention of chronic illnesses. This study aimed to investigate the protective mechanism of yam polysaccharide (CYP) against H<sub>2</sub>O<sub>2</sub>-induced oxidative damage by an RNA-seq technique. The expression of genes and the function of the genome in the process of oxidative damage by H<sub>2</sub>O<sub>2</sub> in IEC-6 cells were explored through transcriptomic analysis. The results illustrated that H<sub>2</sub>O<sub>2</sub> damaged cells by promoting cell differentiation and affecting tight junction proteins, and CYP could achieve cell protection via restraining the activation of the MAPK signaling pathway. RNA-seq analysis revealed that H<sub>2</sub>O<sub>2</sub> may damage cells by promoting the IL-17 signaling pathway and the MAPK signaling pathway and so forth. The Western blot showed that the pretreatment of CYP could restrain the activation of the MAPK signaling pathway. In summary, this study demonstrates that the efficacy of CYP in modulating the MAPK signaling pathway against excessive oxidative stress, with a corresponding preventive role against injury to the intestinal barrier. It provides a new perspective for the understanding of the preventive role of CYP on intestinal damage. These findings suggest that CYP could be used as oxidation protectant and may have potential application prospects in the food and pharmaceutical industries.https://www.mdpi.com/2304-8158/12/2/262intestinal barrieryam polysaccharideRNA-seqoxidative damagesignaling pathways |
| spellingShingle | Mingyue Shen Ruixin Cai Zhedong Li Xiaodie Chen Jianhua Xie The Molecular Mechanism of Yam Polysaccharide Protected H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Damage in IEC-6 Cells Foods intestinal barrier yam polysaccharide RNA-seq oxidative damage signaling pathways |
| title | The Molecular Mechanism of Yam Polysaccharide Protected H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Damage in IEC-6 Cells |
| title_full | The Molecular Mechanism of Yam Polysaccharide Protected H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Damage in IEC-6 Cells |
| title_fullStr | The Molecular Mechanism of Yam Polysaccharide Protected H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Damage in IEC-6 Cells |
| title_full_unstemmed | The Molecular Mechanism of Yam Polysaccharide Protected H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Damage in IEC-6 Cells |
| title_short | The Molecular Mechanism of Yam Polysaccharide Protected H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Damage in IEC-6 Cells |
| title_sort | molecular mechanism of yam polysaccharide protected h sub 2 sub o sub 2 sub induced oxidative damage in iec 6 cells |
| topic | intestinal barrier yam polysaccharide RNA-seq oxidative damage signaling pathways |
| url | https://www.mdpi.com/2304-8158/12/2/262 |
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