Sustainable Methodologies for Efficient Gel Electrophoresis and Streamlined Screening of Difficult Plasmids
We describe a workflow for efficient, environmentally attentive, and sustainable practices related to routine agarose gel electrophoresis. The methods reduce plastic waste and improve efficiency, especially for the exhaustive screening of difficult-to-obtain plasmids. Sustainability is increased whe...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Methods and Protocols |
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| Online Access: | https://www.mdpi.com/2409-9279/6/2/25 |
| _version_ | 1797604106292953088 |
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| author | Nadeem Asad Emily Smith Sudeep Shakya Sutton Stegman Lisa Timmons |
| author_facet | Nadeem Asad Emily Smith Sudeep Shakya Sutton Stegman Lisa Timmons |
| author_sort | Nadeem Asad |
| collection | DOAJ |
| description | We describe a workflow for efficient, environmentally attentive, and sustainable practices related to routine agarose gel electrophoresis. The methods reduce plastic waste and improve efficiency, especially for the exhaustive screening of difficult-to-obtain plasmids. Sustainability is increased when agarose is used ten times over by virtue of a thorough recycling regimen. The workflow optimizes workspaces and standardizes lab practices for handling potentially hazardous waste, minimizing environmental harm. Safety, efficiency, and sustainability improve laboratory productivity, help minimize environmental contamination, and increase cost-effectiveness. |
| first_indexed | 2024-03-11T04:41:37Z |
| format | Article |
| id | doaj.art-8cb78a1832e54f179b573c9c06869931 |
| institution | Directory Open Access Journal |
| issn | 2409-9279 |
| language | English |
| last_indexed | 2024-03-11T04:41:37Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Methods and Protocols |
| spelling | doaj.art-8cb78a1832e54f179b573c9c068699312023-11-17T20:42:02ZengMDPI AGMethods and Protocols2409-92792023-03-01622510.3390/mps6020025Sustainable Methodologies for Efficient Gel Electrophoresis and Streamlined Screening of Difficult PlasmidsNadeem Asad0Emily Smith1Sudeep Shakya2Sutton Stegman3Lisa Timmons4Department of Molecular Biosciences, The University of Kansas, 1200 Sunnyside Avenue, Lawrence, KS 66045, USADepartment of Molecular Biosciences, The University of Kansas, 1200 Sunnyside Avenue, Lawrence, KS 66045, USADepartment of Molecular Biosciences, The University of Kansas, 1200 Sunnyside Avenue, Lawrence, KS 66045, USADepartment of Molecular Biosciences, The University of Kansas, 1200 Sunnyside Avenue, Lawrence, KS 66045, USADepartment of Molecular Biosciences, The University of Kansas, 1200 Sunnyside Avenue, Lawrence, KS 66045, USAWe describe a workflow for efficient, environmentally attentive, and sustainable practices related to routine agarose gel electrophoresis. The methods reduce plastic waste and improve efficiency, especially for the exhaustive screening of difficult-to-obtain plasmids. Sustainability is increased when agarose is used ten times over by virtue of a thorough recycling regimen. The workflow optimizes workspaces and standardizes lab practices for handling potentially hazardous waste, minimizing environmental harm. Safety, efficiency, and sustainability improve laboratory productivity, help minimize environmental contamination, and increase cost-effectiveness.https://www.mdpi.com/2409-9279/6/2/25electrophoresisagaroseethidium bromidesustainability |
| spellingShingle | Nadeem Asad Emily Smith Sudeep Shakya Sutton Stegman Lisa Timmons Sustainable Methodologies for Efficient Gel Electrophoresis and Streamlined Screening of Difficult Plasmids Methods and Protocols electrophoresis agarose ethidium bromide sustainability |
| title | Sustainable Methodologies for Efficient Gel Electrophoresis and Streamlined Screening of Difficult Plasmids |
| title_full | Sustainable Methodologies for Efficient Gel Electrophoresis and Streamlined Screening of Difficult Plasmids |
| title_fullStr | Sustainable Methodologies for Efficient Gel Electrophoresis and Streamlined Screening of Difficult Plasmids |
| title_full_unstemmed | Sustainable Methodologies for Efficient Gel Electrophoresis and Streamlined Screening of Difficult Plasmids |
| title_short | Sustainable Methodologies for Efficient Gel Electrophoresis and Streamlined Screening of Difficult Plasmids |
| title_sort | sustainable methodologies for efficient gel electrophoresis and streamlined screening of difficult plasmids |
| topic | electrophoresis agarose ethidium bromide sustainability |
| url | https://www.mdpi.com/2409-9279/6/2/25 |
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