miR-665-Mediated Regulation of AHCYL2 and BVES Genes in Recurrent Implantation Failure
The primary goal of this investigation was to identify mRNA targets affected by dysregulated miRNAs in RIF. This was accomplished by comprehensively analyzing mRNA and miRNA expression profiles in two groups: female subjects with normal reproductive function (control, n = 5) and female subjects expe...
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| Format: | Article |
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MDPI AG
2024-02-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/15/2/244 |
| _version_ | 1797298151346929664 |
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| author | Sung Hwan Cho Young Myeong Kim Hui Jeong An Ji Hyang Kim Nam Keun Kim |
| author_facet | Sung Hwan Cho Young Myeong Kim Hui Jeong An Ji Hyang Kim Nam Keun Kim |
| author_sort | Sung Hwan Cho |
| collection | DOAJ |
| description | The primary goal of this investigation was to identify mRNA targets affected by dysregulated miRNAs in RIF. This was accomplished by comprehensively analyzing mRNA and miRNA expression profiles in two groups: female subjects with normal reproductive function (control, n = 5) and female subjects experiencing recurrent implantation failure (RIF, n = 5). We conducted transcriptome sequencing and small RNA sequencing on endometrial tissue samples from these cohorts. Subsequently, we validated a selection of intriguing findings using real-time PCR with samples from the same cohort. In total, our analysis revealed that 929 mRNAs exhibited differential expression patterns between the control and RIF patient groups. Notably, our investigation confirmed the significant involvement of dysregulated genes in the context of RIF. Furthermore, we uncovered promising correlation patterns within these mRNA/miRNA pairs. Functional categorization of these miRNA/mRNA pairs highlighted that the differentially expressed genes were predominantly associated with processes such as angiogenesis and cell adhesion. We identified new target genes that are regulated by miR-665, including Blood Vessel Epicardial Substance (BVES) and Adenosylhomocysteinase like 2 (AHCYL2). Our findings suggest that abnormal regulation of genes involved in angiogenesis and cell adhesion, including BVES and AHCYL2, contributes to the endometrial dysfunction observed in women with recurrent implantation failure (RIF) compared to healthy women. |
| first_indexed | 2024-03-07T22:30:43Z |
| format | Article |
| id | doaj.art-8cbb5b132d964a32baf3bc178561130b |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-07T22:30:43Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-8cbb5b132d964a32baf3bc178561130b2024-02-23T15:17:56ZengMDPI AGGenes2073-44252024-02-0115224410.3390/genes15020244miR-665-Mediated Regulation of AHCYL2 and BVES Genes in Recurrent Implantation FailureSung Hwan Cho0Young Myeong Kim1Hui Jeong An2Ji Hyang Kim3Nam Keun Kim4Department of Biomedical Science, College of Life Science, CHA University, Seongnam 13488, Republic of KoreaKangwon Institute of Inclusive Technology, Kangwon National University, Chuncheon 24341, Republic of KoreaDepartment of Biomedical Science, College of Life Science, CHA University, Seongnam 13488, Republic of KoreaDepartment of Obstetrics and Gynecology, CHA Bundang Medical Center, School of Medicine, CHA University, Seongnam 13496, Republic of KoreaDepartment of Biomedical Science, College of Life Science, CHA University, Seongnam 13488, Republic of KoreaThe primary goal of this investigation was to identify mRNA targets affected by dysregulated miRNAs in RIF. This was accomplished by comprehensively analyzing mRNA and miRNA expression profiles in two groups: female subjects with normal reproductive function (control, n = 5) and female subjects experiencing recurrent implantation failure (RIF, n = 5). We conducted transcriptome sequencing and small RNA sequencing on endometrial tissue samples from these cohorts. Subsequently, we validated a selection of intriguing findings using real-time PCR with samples from the same cohort. In total, our analysis revealed that 929 mRNAs exhibited differential expression patterns between the control and RIF patient groups. Notably, our investigation confirmed the significant involvement of dysregulated genes in the context of RIF. Furthermore, we uncovered promising correlation patterns within these mRNA/miRNA pairs. Functional categorization of these miRNA/mRNA pairs highlighted that the differentially expressed genes were predominantly associated with processes such as angiogenesis and cell adhesion. We identified new target genes that are regulated by miR-665, including Blood Vessel Epicardial Substance (BVES) and Adenosylhomocysteinase like 2 (AHCYL2). Our findings suggest that abnormal regulation of genes involved in angiogenesis and cell adhesion, including BVES and AHCYL2, contributes to the endometrial dysfunction observed in women with recurrent implantation failure (RIF) compared to healthy women.https://www.mdpi.com/2073-4425/15/2/244recurrent pregnancy losstranscriptome sequencingsmall RNA sequencingmicroRNA |
| spellingShingle | Sung Hwan Cho Young Myeong Kim Hui Jeong An Ji Hyang Kim Nam Keun Kim miR-665-Mediated Regulation of AHCYL2 and BVES Genes in Recurrent Implantation Failure Genes recurrent pregnancy loss transcriptome sequencing small RNA sequencing microRNA |
| title | miR-665-Mediated Regulation of AHCYL2 and BVES Genes in Recurrent Implantation Failure |
| title_full | miR-665-Mediated Regulation of AHCYL2 and BVES Genes in Recurrent Implantation Failure |
| title_fullStr | miR-665-Mediated Regulation of AHCYL2 and BVES Genes in Recurrent Implantation Failure |
| title_full_unstemmed | miR-665-Mediated Regulation of AHCYL2 and BVES Genes in Recurrent Implantation Failure |
| title_short | miR-665-Mediated Regulation of AHCYL2 and BVES Genes in Recurrent Implantation Failure |
| title_sort | mir 665 mediated regulation of ahcyl2 and bves genes in recurrent implantation failure |
| topic | recurrent pregnancy loss transcriptome sequencing small RNA sequencing microRNA |
| url | https://www.mdpi.com/2073-4425/15/2/244 |
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